Cargando…

Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction

Breast cancer is a major health concern worldwide and is the leading cause of cancer-related death among American women. Traditional therapies, such as surgery, chemotherapy, and radiotherapy, are usually ineffective. Furthermore, cancer recurrence following targeted therapy often results from acqui...

Descripción completa

Detalles Bibliográficos
Autores principales: Rezaeeyazdi, Mahboobeh, Colombani, Thibault, Eggermont, Loek J., Bencherif, Sidi A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844817/
https://www.ncbi.nlm.nih.gov/pubmed/35198956
http://dx.doi.org/10.1016/j.mtbio.2022.100207
_version_ 1784651551031164928
author Rezaeeyazdi, Mahboobeh
Colombani, Thibault
Eggermont, Loek J.
Bencherif, Sidi A.
author_facet Rezaeeyazdi, Mahboobeh
Colombani, Thibault
Eggermont, Loek J.
Bencherif, Sidi A.
author_sort Rezaeeyazdi, Mahboobeh
collection PubMed
description Breast cancer is a major health concern worldwide and is the leading cause of cancer-related death among American women. Traditional therapies, such as surgery, chemotherapy, and radiotherapy, are usually ineffective. Furthermore, cancer recurrence following targeted therapy often results from acquired drug resistance. Therefore, more realistic tumor models than monolayer cell culture for drug screening and discovery in an in vitro setting would facilitate the development of new therapeutic strategies. Toward this goal, we first developed a simple, rapid, low-cost, and high-throughput method for generating uniform multi-cellular tumor spheroids (MCTS) with controllable size. Next, biomimetic cryogel scaffolds fabricated from hyaluronic acid (HA) were utilized as a platform to reconstruct breast tumor microtissues with aspects of the complex tumor microenvironment in three dimensions. Finally, we investigated the interactions between the HA-based cryogels and CD44-positive breast tumor cells, individually or as MCTS. We found that incorporating the adhesive RGD peptide in cryogels led to the formation of a monolayer of tumor cells on the polymer walls, whereas MCTS cultured on RGD-free HA cryogels resulted in the growth of large and dense microtumors, more similar to native tumor masses. As a result, the MCTS-laden HA cryogel system induced a highly aggressive and chemotherapy drug-resistant tumor model. RGD-free HA-based cryogels represent an effective starting point for designing tumor models for preclinical research, therapeutic drug screening, and early cancer diagnosis.
format Online
Article
Text
id pubmed-8844817
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-88448172022-02-22 Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction Rezaeeyazdi, Mahboobeh Colombani, Thibault Eggermont, Loek J. Bencherif, Sidi A. Mater Today Bio Full Length Article Breast cancer is a major health concern worldwide and is the leading cause of cancer-related death among American women. Traditional therapies, such as surgery, chemotherapy, and radiotherapy, are usually ineffective. Furthermore, cancer recurrence following targeted therapy often results from acquired drug resistance. Therefore, more realistic tumor models than monolayer cell culture for drug screening and discovery in an in vitro setting would facilitate the development of new therapeutic strategies. Toward this goal, we first developed a simple, rapid, low-cost, and high-throughput method for generating uniform multi-cellular tumor spheroids (MCTS) with controllable size. Next, biomimetic cryogel scaffolds fabricated from hyaluronic acid (HA) were utilized as a platform to reconstruct breast tumor microtissues with aspects of the complex tumor microenvironment in three dimensions. Finally, we investigated the interactions between the HA-based cryogels and CD44-positive breast tumor cells, individually or as MCTS. We found that incorporating the adhesive RGD peptide in cryogels led to the formation of a monolayer of tumor cells on the polymer walls, whereas MCTS cultured on RGD-free HA cryogels resulted in the growth of large and dense microtumors, more similar to native tumor masses. As a result, the MCTS-laden HA cryogel system induced a highly aggressive and chemotherapy drug-resistant tumor model. RGD-free HA-based cryogels represent an effective starting point for designing tumor models for preclinical research, therapeutic drug screening, and early cancer diagnosis. Elsevier 2022-01-24 /pmc/articles/PMC8844817/ /pubmed/35198956 http://dx.doi.org/10.1016/j.mtbio.2022.100207 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Full Length Article
Rezaeeyazdi, Mahboobeh
Colombani, Thibault
Eggermont, Loek J.
Bencherif, Sidi A.
Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title_full Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title_fullStr Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title_full_unstemmed Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title_short Engineering hyaluronic acid-based cryogels for CD44-mediated breast tumor reconstruction
title_sort engineering hyaluronic acid-based cryogels for cd44-mediated breast tumor reconstruction
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844817/
https://www.ncbi.nlm.nih.gov/pubmed/35198956
http://dx.doi.org/10.1016/j.mtbio.2022.100207
work_keys_str_mv AT rezaeeyazdimahboobeh engineeringhyaluronicacidbasedcryogelsforcd44mediatedbreasttumorreconstruction
AT colombanithibault engineeringhyaluronicacidbasedcryogelsforcd44mediatedbreasttumorreconstruction
AT eggermontloekj engineeringhyaluronicacidbasedcryogelsforcd44mediatedbreasttumorreconstruction
AT bencherifsidia engineeringhyaluronicacidbasedcryogelsforcd44mediatedbreasttumorreconstruction