Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation

Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca(2+)) signals, but available protocols for neuronal Ca2(+) imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca(2+) imaging in C. elegans following three different ap...

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Autores principales: Cheng, Hankui, Al-Sheikh, Umar, Chen, Du, Duan, Duo, Kang, Lijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844900/
https://www.ncbi.nlm.nih.gov/pubmed/35199034
http://dx.doi.org/10.1016/j.xpro.2022.101169
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author Cheng, Hankui
Al-Sheikh, Umar
Chen, Du
Duan, Duo
Kang, Lijun
author_facet Cheng, Hankui
Al-Sheikh, Umar
Chen, Du
Duan, Duo
Kang, Lijun
author_sort Cheng, Hankui
collection PubMed
description Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca(2+)) signals, but available protocols for neuronal Ca2(+) imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca(2+) imaging in C. elegans following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan et al. (2020).
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spelling pubmed-88449002022-02-22 Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation Cheng, Hankui Al-Sheikh, Umar Chen, Du Duan, Duo Kang, Lijun STAR Protoc Protocol Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca(2+)) signals, but available protocols for neuronal Ca2(+) imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca(2+) imaging in C. elegans following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan et al. (2020). Elsevier 2022-02-10 /pmc/articles/PMC8844900/ /pubmed/35199034 http://dx.doi.org/10.1016/j.xpro.2022.101169 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Cheng, Hankui
Al-Sheikh, Umar
Chen, Du
Duan, Duo
Kang, Lijun
Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title_full Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title_fullStr Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title_full_unstemmed Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title_short Protocol for glial Ca(2+) imaging in C. elegans following chemical, mechanical, or optogenetic stimulation
title_sort protocol for glial ca(2+) imaging in c. elegans following chemical, mechanical, or optogenetic stimulation
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844900/
https://www.ncbi.nlm.nih.gov/pubmed/35199034
http://dx.doi.org/10.1016/j.xpro.2022.101169
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