Identification and three-dimensional reconstruction of oxytocin receptor expressing astrocytes in the rat and mouse brain

Here, we present a step-by-step protocol for three-dimensional reconstruction of astrocyte morphology, applied to the central amygdala oxytocin receptor-expressing astrocytes. This includes RNAse-free perfusion, combination of RNAscope and immunohistochemistry, and confocal imaging. This protocol pr...

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Detalles Bibliográficos
Autores principales: Althammer, Ferdinand, Krause, Eric G., de Kloet, Anette D., Smith, Justin, Grinevich, Valery, Charlet, Alexandre, Stern, Javier E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844904/
https://www.ncbi.nlm.nih.gov/pubmed/35199030
http://dx.doi.org/10.1016/j.xpro.2022.101160

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