Cargando…
Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts
Replicative aging is an underexplored field of research in medical mycology. Cryptococcus neoformans (Cn) and Candida glabrata (Cg) are dreaded fungal pathogens that cause fatal invasive infections. The fungal cell wall is essential for yeast viability and pathogenesis. In this study, we provide dat...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844920/ https://www.ncbi.nlm.nih.gov/pubmed/35164553 http://dx.doi.org/10.1128/mbio.00190-22 |
_version_ | 1784651570295603200 |
---|---|
author | Silva, Vanessa K. A. Bhattacharya, Somanon Oliveira, Natalia Kronbauer Savitt, Anne G. Zamith-Miranda, Daniel Nosanchuk, Joshua D. Fries, Bettina C. |
author_facet | Silva, Vanessa K. A. Bhattacharya, Somanon Oliveira, Natalia Kronbauer Savitt, Anne G. Zamith-Miranda, Daniel Nosanchuk, Joshua D. Fries, Bettina C. |
author_sort | Silva, Vanessa K. A. |
collection | PubMed |
description | Replicative aging is an underexplored field of research in medical mycology. Cryptococcus neoformans (Cn) and Candida glabrata (Cg) are dreaded fungal pathogens that cause fatal invasive infections. The fungal cell wall is essential for yeast viability and pathogenesis. In this study, we provide data characterizing age-associated modifications to the cell wall of Cn and Cg. Here, we report that old yeast cells upregulate genes of cell wall biosynthesis, leading to cell wall reorganization and increased levels of all major components, including glucan, chitin, and its derivatives, as well as mannan. This results in a significant thickening of the cell wall in aged cells. Old-generation yeast cells exhibited drastic ultrastructural changes, including the presence of abundant vesicle-like particles in the cytoplasm, and enlarged vacuoles with altered pH homeostasis. Our findings suggest that the cell wall modifications could be enabled by augmented intracellular trafficking. This work furthers our understanding of the cell phenotype that emerges during aging. It highlights differences in these two fungal pathogens and elucidates mechanisms that explain the enhanced resistance of old cells to antifungals and phagocytic attacks. |
format | Online Article Text |
id | pubmed-8844920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-88449202022-02-17 Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts Silva, Vanessa K. A. Bhattacharya, Somanon Oliveira, Natalia Kronbauer Savitt, Anne G. Zamith-Miranda, Daniel Nosanchuk, Joshua D. Fries, Bettina C. mBio Research Article Replicative aging is an underexplored field of research in medical mycology. Cryptococcus neoformans (Cn) and Candida glabrata (Cg) are dreaded fungal pathogens that cause fatal invasive infections. The fungal cell wall is essential for yeast viability and pathogenesis. In this study, we provide data characterizing age-associated modifications to the cell wall of Cn and Cg. Here, we report that old yeast cells upregulate genes of cell wall biosynthesis, leading to cell wall reorganization and increased levels of all major components, including glucan, chitin, and its derivatives, as well as mannan. This results in a significant thickening of the cell wall in aged cells. Old-generation yeast cells exhibited drastic ultrastructural changes, including the presence of abundant vesicle-like particles in the cytoplasm, and enlarged vacuoles with altered pH homeostasis. Our findings suggest that the cell wall modifications could be enabled by augmented intracellular trafficking. This work furthers our understanding of the cell phenotype that emerges during aging. It highlights differences in these two fungal pathogens and elucidates mechanisms that explain the enhanced resistance of old cells to antifungals and phagocytic attacks. American Society for Microbiology 2022-02-15 /pmc/articles/PMC8844920/ /pubmed/35164553 http://dx.doi.org/10.1128/mbio.00190-22 Text en https://doi.org/10.1128/AuthorWarrantyLicense.v1This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply. |
spellingShingle | Research Article Silva, Vanessa K. A. Bhattacharya, Somanon Oliveira, Natalia Kronbauer Savitt, Anne G. Zamith-Miranda, Daniel Nosanchuk, Joshua D. Fries, Bettina C. Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title | Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title_full | Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title_fullStr | Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title_full_unstemmed | Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title_short | Replicative Aging Remodels the Cell Wall and Is Associated with Increased Intracellular Trafficking in Human Pathogenic Yeasts |
title_sort | replicative aging remodels the cell wall and is associated with increased intracellular trafficking in human pathogenic yeasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844920/ https://www.ncbi.nlm.nih.gov/pubmed/35164553 http://dx.doi.org/10.1128/mbio.00190-22 |
work_keys_str_mv | AT silvavanessaka replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT bhattacharyasomanon replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT oliveiranataliakronbauer replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT savittanneg replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT zamithmirandadaniel replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT nosanchukjoshuad replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts AT friesbettinac replicativeagingremodelsthecellwallandisassociatedwithincreasedintracellulartraffickinginhumanpathogenicyeasts |