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Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology

Urogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underly...

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Autores principales: Osakunor, Derick N. M., Ishida, Kenji, Lamanna, Olivia K., Rossi, Mario, Dwomoh, Louis, Hsieh, Michael H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846513/
https://www.ncbi.nlm.nih.gov/pubmed/35167594
http://dx.doi.org/10.1371/journal.pntd.0010176
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author Osakunor, Derick N. M.
Ishida, Kenji
Lamanna, Olivia K.
Rossi, Mario
Dwomoh, Louis
Hsieh, Michael H.
author_facet Osakunor, Derick N. M.
Ishida, Kenji
Lamanna, Olivia K.
Rossi, Mario
Dwomoh, Louis
Hsieh, Michael H.
author_sort Osakunor, Derick N. M.
collection PubMed
description Urogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology are not well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology. Purified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry. We demonstrate that biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism were significantly enriched in S. haematobium infection. S. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Our findings highlight the potential bladder protein indicators for host-parasite interplay and provide new insights into the complex dynamics of pathology and characteristic bladder tissue changes in urogenital schistosomiasis. The findings will be relevant for development of improved interventions for disease control.
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spelling pubmed-88465132022-02-16 Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology Osakunor, Derick N. M. Ishida, Kenji Lamanna, Olivia K. Rossi, Mario Dwomoh, Louis Hsieh, Michael H. PLoS Negl Trop Dis Research Article Urogenital schistosomiasis remains a major public health concern worldwide. In response to egg deposition, the host bladder undergoes gross and molecular morphological changes relevant for disease manifestation. However, limited mechanistic studies to date imply that the molecular mechanisms underlying pathology are not well-defined. We leveraged a mouse model of urogenital schistosomiasis to perform for the first time, proteome profiling of the early molecular events that occur in the bladder after exposure to S. haematobium eggs, and to elucidate the protein pathways involved in urogenital schistosomiasis-induced pathology. Purified S. haematobium eggs or control vehicle were microinjected into the bladder walls of mice. Mice were sacrificed seven days post-injection and bladder proteins isolated and processed for proteome profiling using mass spectrometry. We demonstrate that biological processes including carcinogenesis, immune and inflammatory responses, increased protein translation or turnover, oxidative stress responses, reduced cell adhesion and epithelial barrier integrity, and increased glucose metabolism were significantly enriched in S. haematobium infection. S. haematobium egg deposition in the bladder results in significant changes in proteins and pathways that play a role in pathology. Our findings highlight the potential bladder protein indicators for host-parasite interplay and provide new insights into the complex dynamics of pathology and characteristic bladder tissue changes in urogenital schistosomiasis. The findings will be relevant for development of improved interventions for disease control. Public Library of Science 2022-02-15 /pmc/articles/PMC8846513/ /pubmed/35167594 http://dx.doi.org/10.1371/journal.pntd.0010176 Text en © 2022 Osakunor et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Osakunor, Derick N. M.
Ishida, Kenji
Lamanna, Olivia K.
Rossi, Mario
Dwomoh, Louis
Hsieh, Michael H.
Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title_full Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title_fullStr Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title_full_unstemmed Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title_short Host tissue proteomics reveal insights into the molecular basis of Schistosoma haematobium-induced bladder pathology
title_sort host tissue proteomics reveal insights into the molecular basis of schistosoma haematobium-induced bladder pathology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846513/
https://www.ncbi.nlm.nih.gov/pubmed/35167594
http://dx.doi.org/10.1371/journal.pntd.0010176
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