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Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature
The occurrence of MYC-negative Burkitt lymphoma (BL) has been discussed for many years. The real frequency of the MYC insertion in MYC-negative BL is still unknown. Fine-needle aspiration biopsies of 108 consecutive patients with clinicopathologically suspected BL (suspBL) were evaluated by flow cyt...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846522/ https://www.ncbi.nlm.nih.gov/pubmed/35167621 http://dx.doi.org/10.1371/journal.pone.0263980 |
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author | Woroniecka, Renata Rymkiewicz, Grzegorz Szafron, Lukasz M. Blachnio, Katarzyna Szafron, Laura A. Bystydzienski, Zbigniew Pienkowska-Grela, Barbara Borkowska, Klaudia Rygier, Jolanta Kotyl, Aleksandra Malawska, Natalia Wojtkowska, Katarzyna Parada, Joanna Borysiuk, Anita Murcia Pienkowski, Victor Rydzanicz, Malgorzata Grygalewicz, Beata |
author_facet | Woroniecka, Renata Rymkiewicz, Grzegorz Szafron, Lukasz M. Blachnio, Katarzyna Szafron, Laura A. Bystydzienski, Zbigniew Pienkowska-Grela, Barbara Borkowska, Klaudia Rygier, Jolanta Kotyl, Aleksandra Malawska, Natalia Wojtkowska, Katarzyna Parada, Joanna Borysiuk, Anita Murcia Pienkowski, Victor Rydzanicz, Malgorzata Grygalewicz, Beata |
author_sort | Woroniecka, Renata |
collection | PubMed |
description | The occurrence of MYC-negative Burkitt lymphoma (BL) has been discussed for many years. The real frequency of the MYC insertion in MYC-negative BL is still unknown. Fine-needle aspiration biopsies of 108 consecutive patients with clinicopathologically suspected BL (suspBL) were evaluated by flow cytometry, classical cytogenetics, and fluorescence in situ hybridization (FISH). We found 12 cases (11%) without the MYC rearrangement by FISH with a MYC breakapart probe: two patients (1.9%) with cryptic MYC/IGH fusion (finally diagnosed as BL) and 10 patients (9.3%) with 11q gain/loss (finally diagnosed as Burkitt-like lymphoma with 11q aberration). The exact breakpoints of the cryptic MYC/IGH were investigated by next-generation sequencing. The MYC insertions’ breakpoints were identified in PVT1 in the first case, and 42 kb upstream of 5′MYC in the second case. To date, a molecular characterization of the MYC insertion in BL has only been reported in one case. Detailed descriptions of our MYC insertions in a routinely and consecutively diagnosed suspBL cohort will contribute to resolving the issue of MYC negativity in BL. In our opinion, the presence of the MYC insertions in BL and other lymphomas might be underestimated, because routine genetic diagnostics are usually based on FISH only, without karyotyping. |
format | Online Article Text |
id | pubmed-8846522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-88465222022-02-16 Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature Woroniecka, Renata Rymkiewicz, Grzegorz Szafron, Lukasz M. Blachnio, Katarzyna Szafron, Laura A. Bystydzienski, Zbigniew Pienkowska-Grela, Barbara Borkowska, Klaudia Rygier, Jolanta Kotyl, Aleksandra Malawska, Natalia Wojtkowska, Katarzyna Parada, Joanna Borysiuk, Anita Murcia Pienkowski, Victor Rydzanicz, Malgorzata Grygalewicz, Beata PLoS One Research Article The occurrence of MYC-negative Burkitt lymphoma (BL) has been discussed for many years. The real frequency of the MYC insertion in MYC-negative BL is still unknown. Fine-needle aspiration biopsies of 108 consecutive patients with clinicopathologically suspected BL (suspBL) were evaluated by flow cytometry, classical cytogenetics, and fluorescence in situ hybridization (FISH). We found 12 cases (11%) without the MYC rearrangement by FISH with a MYC breakapart probe: two patients (1.9%) with cryptic MYC/IGH fusion (finally diagnosed as BL) and 10 patients (9.3%) with 11q gain/loss (finally diagnosed as Burkitt-like lymphoma with 11q aberration). The exact breakpoints of the cryptic MYC/IGH were investigated by next-generation sequencing. The MYC insertions’ breakpoints were identified in PVT1 in the first case, and 42 kb upstream of 5′MYC in the second case. To date, a molecular characterization of the MYC insertion in BL has only been reported in one case. Detailed descriptions of our MYC insertions in a routinely and consecutively diagnosed suspBL cohort will contribute to resolving the issue of MYC negativity in BL. In our opinion, the presence of the MYC insertions in BL and other lymphomas might be underestimated, because routine genetic diagnostics are usually based on FISH only, without karyotyping. Public Library of Science 2022-02-15 /pmc/articles/PMC8846522/ /pubmed/35167621 http://dx.doi.org/10.1371/journal.pone.0263980 Text en © 2022 Woroniecka et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Woroniecka, Renata Rymkiewicz, Grzegorz Szafron, Lukasz M. Blachnio, Katarzyna Szafron, Laura A. Bystydzienski, Zbigniew Pienkowska-Grela, Barbara Borkowska, Klaudia Rygier, Jolanta Kotyl, Aleksandra Malawska, Natalia Wojtkowska, Katarzyna Parada, Joanna Borysiuk, Anita Murcia Pienkowski, Victor Rydzanicz, Malgorzata Grygalewicz, Beata Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title | Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title_full | Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title_fullStr | Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title_full_unstemmed | Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title_short | Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the literature |
title_sort | cryptic myc insertions in burkitt lymphoma: new data and a review of the literature |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846522/ https://www.ncbi.nlm.nih.gov/pubmed/35167621 http://dx.doi.org/10.1371/journal.pone.0263980 |
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