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Assessing the effects of stress on feeding behaviors in laboratory mice

Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay...

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Autores principales: Francois, Marie, Canal Delgado, Isabella, Shargorodsky, Nikolay, Leu, Cheng-Shiun, Zeltser, Lori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846584/
https://www.ncbi.nlm.nih.gov/pubmed/35167441
http://dx.doi.org/10.7554/eLife.70271
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author Francois, Marie
Canal Delgado, Isabella
Shargorodsky, Nikolay
Leu, Cheng-Shiun
Zeltser, Lori
author_facet Francois, Marie
Canal Delgado, Isabella
Shargorodsky, Nikolay
Leu, Cheng-Shiun
Zeltser, Lori
author_sort Francois, Marie
collection PubMed
description Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents.
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spelling pubmed-88465842022-02-16 Assessing the effects of stress on feeding behaviors in laboratory mice Francois, Marie Canal Delgado, Isabella Shargorodsky, Nikolay Leu, Cheng-Shiun Zeltser, Lori eLife Neuroscience Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents. eLife Sciences Publications, Ltd 2022-02-15 /pmc/articles/PMC8846584/ /pubmed/35167441 http://dx.doi.org/10.7554/eLife.70271 Text en © 2022, Francois et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Francois, Marie
Canal Delgado, Isabella
Shargorodsky, Nikolay
Leu, Cheng-Shiun
Zeltser, Lori
Assessing the effects of stress on feeding behaviors in laboratory mice
title Assessing the effects of stress on feeding behaviors in laboratory mice
title_full Assessing the effects of stress on feeding behaviors in laboratory mice
title_fullStr Assessing the effects of stress on feeding behaviors in laboratory mice
title_full_unstemmed Assessing the effects of stress on feeding behaviors in laboratory mice
title_short Assessing the effects of stress on feeding behaviors in laboratory mice
title_sort assessing the effects of stress on feeding behaviors in laboratory mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846584/
https://www.ncbi.nlm.nih.gov/pubmed/35167441
http://dx.doi.org/10.7554/eLife.70271
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