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Assessing the effects of stress on feeding behaviors in laboratory mice
Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846584/ https://www.ncbi.nlm.nih.gov/pubmed/35167441 http://dx.doi.org/10.7554/eLife.70271 |
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author | Francois, Marie Canal Delgado, Isabella Shargorodsky, Nikolay Leu, Cheng-Shiun Zeltser, Lori |
author_facet | Francois, Marie Canal Delgado, Isabella Shargorodsky, Nikolay Leu, Cheng-Shiun Zeltser, Lori |
author_sort | Francois, Marie |
collection | PubMed |
description | Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents. |
format | Online Article Text |
id | pubmed-8846584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-88465842022-02-16 Assessing the effects of stress on feeding behaviors in laboratory mice Francois, Marie Canal Delgado, Isabella Shargorodsky, Nikolay Leu, Cheng-Shiun Zeltser, Lori eLife Neuroscience Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents. eLife Sciences Publications, Ltd 2022-02-15 /pmc/articles/PMC8846584/ /pubmed/35167441 http://dx.doi.org/10.7554/eLife.70271 Text en © 2022, Francois et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Francois, Marie Canal Delgado, Isabella Shargorodsky, Nikolay Leu, Cheng-Shiun Zeltser, Lori Assessing the effects of stress on feeding behaviors in laboratory mice |
title | Assessing the effects of stress on feeding behaviors in laboratory mice |
title_full | Assessing the effects of stress on feeding behaviors in laboratory mice |
title_fullStr | Assessing the effects of stress on feeding behaviors in laboratory mice |
title_full_unstemmed | Assessing the effects of stress on feeding behaviors in laboratory mice |
title_short | Assessing the effects of stress on feeding behaviors in laboratory mice |
title_sort | assessing the effects of stress on feeding behaviors in laboratory mice |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846584/ https://www.ncbi.nlm.nih.gov/pubmed/35167441 http://dx.doi.org/10.7554/eLife.70271 |
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