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A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation

In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded precursors of ~30 bp that are synthesized by NUCLEA...

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Autores principales: Loffer, Andrew, Singh, Jasleen, Fukudome, Akihito, Mishra, Vibhor, Wang, Feng, Pikaard, Craig S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846587/
https://www.ncbi.nlm.nih.gov/pubmed/35098919
http://dx.doi.org/10.7554/eLife.73260
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author Loffer, Andrew
Singh, Jasleen
Fukudome, Akihito
Mishra, Vibhor
Wang, Feng
Pikaard, Craig S
author_facet Loffer, Andrew
Singh, Jasleen
Fukudome, Akihito
Mishra, Vibhor
Wang, Feng
Pikaard, Craig S
author_sort Loffer, Andrew
collection PubMed
description In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded precursors of ~30 bp that are synthesized by NUCLEAR RNA POLYMERASE IV (Pol IV) and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2). We show that Pol IV’s choice of initiating nucleotide, RDR2’s initiation 1–2 nt internal to Pol IV transcript ends and RDR2’s terminal transferase activity collectively yield a code that influences which precursor end is diced and whether 24 or 23 nt siRNAs are produced. By diversifying the size, sequence, and strand specificity of siRNAs derived from a given precursor, alternative patterns of DCL3 dicing allow for maximal siRNA coverage at methylated target loci.
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spelling pubmed-88465872022-02-16 A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation Loffer, Andrew Singh, Jasleen Fukudome, Akihito Mishra, Vibhor Wang, Feng Pikaard, Craig S eLife Chromosomes and Gene Expression In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded precursors of ~30 bp that are synthesized by NUCLEAR RNA POLYMERASE IV (Pol IV) and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2). We show that Pol IV’s choice of initiating nucleotide, RDR2’s initiation 1–2 nt internal to Pol IV transcript ends and RDR2’s terminal transferase activity collectively yield a code that influences which precursor end is diced and whether 24 or 23 nt siRNAs are produced. By diversifying the size, sequence, and strand specificity of siRNAs derived from a given precursor, alternative patterns of DCL3 dicing allow for maximal siRNA coverage at methylated target loci. eLife Sciences Publications, Ltd 2022-01-31 /pmc/articles/PMC8846587/ /pubmed/35098919 http://dx.doi.org/10.7554/eLife.73260 Text en © 2022, Loffer et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Chromosomes and Gene Expression
Loffer, Andrew
Singh, Jasleen
Fukudome, Akihito
Mishra, Vibhor
Wang, Feng
Pikaard, Craig S
A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title_full A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title_fullStr A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title_full_unstemmed A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title_short A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation
title_sort dcl3 dicing code within pol iv-rdr2 transcripts diversifies the sirna pool guiding rna-directed dna methylation
topic Chromosomes and Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846587/
https://www.ncbi.nlm.nih.gov/pubmed/35098919
http://dx.doi.org/10.7554/eLife.73260
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