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Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle

We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that the fly head cuticle has surprisingly high transmission at wavelengths >900nm, and the difficulty of through-cuticle imaging is due to the...

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Autores principales: Aragon, Max Jameson, Mok, Aaron T, Shea, Jamien, Wang, Mengran, Kim, Haein, Barkdull, Nathan, Xu, Chris, Yapici, Nilay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846588/
https://www.ncbi.nlm.nih.gov/pubmed/35073257
http://dx.doi.org/10.7554/eLife.69094
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author Aragon, Max Jameson
Mok, Aaron T
Shea, Jamien
Wang, Mengran
Kim, Haein
Barkdull, Nathan
Xu, Chris
Yapici, Nilay
author_facet Aragon, Max Jameson
Mok, Aaron T
Shea, Jamien
Wang, Mengran
Kim, Haein
Barkdull, Nathan
Xu, Chris
Yapici, Nilay
author_sort Aragon, Max Jameson
collection PubMed
description We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that the fly head cuticle has surprisingly high transmission at wavelengths >900nm, and the difficulty of through-cuticle imaging is due to the air sacs and/or fat tissue underneath the head cuticle. By compressing or removing the air sacs, we performed multiphoton imaging of the fly brain through the intact cuticle. Our anatomical and functional imaging results show that 2- and 3-photon imaging are comparable in superficial regions such as the mushroom body, but 3-photon imaging is superior in deeper regions such as the central complex and beyond. We further demonstrated 2-photon through-cuticle functional imaging of odor-evoked calcium responses from the mushroom body γ-lobes in behaving flies short term and long term. The through-cuticle imaging method developed here extends the time limits of in vivo imaging in flies and opens new ways to capture neural structure and activity from the fly brain.
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spelling pubmed-88465882022-02-16 Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle Aragon, Max Jameson Mok, Aaron T Shea, Jamien Wang, Mengran Kim, Haein Barkdull, Nathan Xu, Chris Yapici, Nilay eLife Neuroscience We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that the fly head cuticle has surprisingly high transmission at wavelengths >900nm, and the difficulty of through-cuticle imaging is due to the air sacs and/or fat tissue underneath the head cuticle. By compressing or removing the air sacs, we performed multiphoton imaging of the fly brain through the intact cuticle. Our anatomical and functional imaging results show that 2- and 3-photon imaging are comparable in superficial regions such as the mushroom body, but 3-photon imaging is superior in deeper regions such as the central complex and beyond. We further demonstrated 2-photon through-cuticle functional imaging of odor-evoked calcium responses from the mushroom body γ-lobes in behaving flies short term and long term. The through-cuticle imaging method developed here extends the time limits of in vivo imaging in flies and opens new ways to capture neural structure and activity from the fly brain. eLife Sciences Publications, Ltd 2022-01-24 /pmc/articles/PMC8846588/ /pubmed/35073257 http://dx.doi.org/10.7554/eLife.69094 Text en © 2022, Aragon et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Aragon, Max Jameson
Mok, Aaron T
Shea, Jamien
Wang, Mengran
Kim, Haein
Barkdull, Nathan
Xu, Chris
Yapici, Nilay
Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title_full Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title_fullStr Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title_full_unstemmed Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title_short Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle
title_sort multiphoton imaging of neural structure and activity in drosophila through the intact cuticle
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846588/
https://www.ncbi.nlm.nih.gov/pubmed/35073257
http://dx.doi.org/10.7554/eLife.69094
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