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Expression of the foraging gene in adult Drosophila melanogaster

The foraging gene in Drosophila melanogaster, which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult foraging expression is less well characterized than in the larva....

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Autores principales: Allen, Aaron M., B. Sokolowski, Marla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846931/
https://www.ncbi.nlm.nih.gov/pubmed/34382904
http://dx.doi.org/10.1080/01677063.2021.1941946
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author Allen, Aaron M.
B. Sokolowski, Marla
author_facet Allen, Aaron M.
B. Sokolowski, Marla
author_sort Allen, Aaron M.
collection PubMed
description The foraging gene in Drosophila melanogaster, which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult foraging expression is less well characterized than in the larva. We characterized foraging expression in the brain, gastric system, and reproductive systems using a T2A-Gal4 gene-trap allele. In the brain, foraging expression appears to be restricted to multiple sub-types of glia. This glial-specific cellular localization of foraging was supported by single-cell transcriptomic atlases of the adult brain. foraging is extensively expressed in most cell types in the gastric and reproductive systems. We then mapped multiple cis-regulatory elements responsible for parts of the observed expression patterns by a nested cloned promoter-Gal4 analysis. The mapped cis-regulatory elements were consistently modular when comparing the larval and adult expression patterns. These new data using the T2A-Gal4 gene-trap and cloned foraging promoter fusion GAL4’s are discussed with respect to previous work using an anti-FOR antibody, which we show here to be non-specific. Future studies of foraging’s function will consider roles for glial subtypes and peripheral tissues (gastric and reproductive systems) in foraging’s pleiotropic behavioral and physiological effects.
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spelling pubmed-88469312022-02-16 Expression of the foraging gene in adult Drosophila melanogaster Allen, Aaron M. B. Sokolowski, Marla J Neurogenet Original Research Articles The foraging gene in Drosophila melanogaster, which encodes a cGMP-dependent protein kinase, is a highly conserved, complex gene with multiple pleiotropic behavioral and physiological functions in both the larval and adult fly. Adult foraging expression is less well characterized than in the larva. We characterized foraging expression in the brain, gastric system, and reproductive systems using a T2A-Gal4 gene-trap allele. In the brain, foraging expression appears to be restricted to multiple sub-types of glia. This glial-specific cellular localization of foraging was supported by single-cell transcriptomic atlases of the adult brain. foraging is extensively expressed in most cell types in the gastric and reproductive systems. We then mapped multiple cis-regulatory elements responsible for parts of the observed expression patterns by a nested cloned promoter-Gal4 analysis. The mapped cis-regulatory elements were consistently modular when comparing the larval and adult expression patterns. These new data using the T2A-Gal4 gene-trap and cloned foraging promoter fusion GAL4’s are discussed with respect to previous work using an anti-FOR antibody, which we show here to be non-specific. Future studies of foraging’s function will consider roles for glial subtypes and peripheral tissues (gastric and reproductive systems) in foraging’s pleiotropic behavioral and physiological effects. Taylor & Francis 2021-08-12 /pmc/articles/PMC8846931/ /pubmed/34382904 http://dx.doi.org/10.1080/01677063.2021.1941946 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Articles
Allen, Aaron M.
B. Sokolowski, Marla
Expression of the foraging gene in adult Drosophila melanogaster
title Expression of the foraging gene in adult Drosophila melanogaster
title_full Expression of the foraging gene in adult Drosophila melanogaster
title_fullStr Expression of the foraging gene in adult Drosophila melanogaster
title_full_unstemmed Expression of the foraging gene in adult Drosophila melanogaster
title_short Expression of the foraging gene in adult Drosophila melanogaster
title_sort expression of the foraging gene in adult drosophila melanogaster
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846931/
https://www.ncbi.nlm.nih.gov/pubmed/34382904
http://dx.doi.org/10.1080/01677063.2021.1941946
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