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Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States

Enterococcus faecalis and faecium with resistance to daptomycin and/or linezolid are emerging globally. We present the genomic characterization of daptomycin- and linezolid-resistant E. faecalis and E. faecium surveillance isolates from the United States, 2013–2016. Daptomycin resistance was low amo...

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Autores principales: Gargis, Amy S., Spicer, Lori M., Kent, Alyssa G., Zhu, Wenming, Campbell, Davina, McAllister, Gillian, Ewing, Thomas O., Albrecht, Valerie, Stevens, Valerie A., Sheth, Mili, Padilla, Jasmine, Batra, Dhwani, Johnson, J. Kristie, Halpin, Alison Laufer, Rasheed, J. Kamile, Elkins, Christopher A., Karlsson, Maria, Lutgring, Joseph D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846945/
https://www.ncbi.nlm.nih.gov/pubmed/35178041
http://dx.doi.org/10.3389/fmicb.2021.807398
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author Gargis, Amy S.
Spicer, Lori M.
Kent, Alyssa G.
Zhu, Wenming
Campbell, Davina
McAllister, Gillian
Ewing, Thomas O.
Albrecht, Valerie
Stevens, Valerie A.
Sheth, Mili
Padilla, Jasmine
Batra, Dhwani
Johnson, J. Kristie
Halpin, Alison Laufer
Rasheed, J. Kamile
Elkins, Christopher A.
Karlsson, Maria
Lutgring, Joseph D.
author_facet Gargis, Amy S.
Spicer, Lori M.
Kent, Alyssa G.
Zhu, Wenming
Campbell, Davina
McAllister, Gillian
Ewing, Thomas O.
Albrecht, Valerie
Stevens, Valerie A.
Sheth, Mili
Padilla, Jasmine
Batra, Dhwani
Johnson, J. Kristie
Halpin, Alison Laufer
Rasheed, J. Kamile
Elkins, Christopher A.
Karlsson, Maria
Lutgring, Joseph D.
author_sort Gargis, Amy S.
collection PubMed
description Enterococcus faecalis and faecium with resistance to daptomycin and/or linezolid are emerging globally. We present the genomic characterization of daptomycin- and linezolid-resistant E. faecalis and E. faecium surveillance isolates from the United States, 2013–2016. Daptomycin resistance was low among E. faecalis (2/364, 0.5%) and E. faecium (17/344, 5%). The majority (71%, 12/17) of daptomycin-resistant E. faecium isolates belonged to the emerging ST736 clone and contained mutations in liaFSR and cls previously associated with resistance. However, 1/2 E. faecalis and 3/17 E. faecium did not contain these mutations previously associated with daptomycin resistance. Linezolid resistance was rare among E. faecalis (1/364, 0.3%) and E. faecium (2/344, 0.6%). These two E. faecium isolates, one of which was also resistant to daptomycin and vancomycin, contained the 23S rRNA nucleotide mutation (G2576T) associated with linezolid resistance. Long-read sequencing revealed the linezolid-resistant E. faecalis isolate contained chromosomal- and plasmid-encoded copies of optrA. The chromosomal optrA was located on the recently described Tn6674 multiresistance transposon. The second copy of optrA was encoded on an ∼65 kb mosaic plasmid, with component regions sharing high sequence identity to optrA-encoding multiresistance plasmids of animal origin. The optrA-encoding plasmid contained open reading frames predicted to encode proteins associated with a pheromone-responsive plasmid transfer system, and filter mating experiments confirmed the plasmid was conjugative. Continued surveillance of enterococci is necessary to assess the prevalence and trends of daptomycin and linezolid resistance in the United States, characterize resistance mechanisms and how they transfer, and monitor for emerging sequence types associated with resistance.
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spelling pubmed-88469452022-02-16 Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States Gargis, Amy S. Spicer, Lori M. Kent, Alyssa G. Zhu, Wenming Campbell, Davina McAllister, Gillian Ewing, Thomas O. Albrecht, Valerie Stevens, Valerie A. Sheth, Mili Padilla, Jasmine Batra, Dhwani Johnson, J. Kristie Halpin, Alison Laufer Rasheed, J. Kamile Elkins, Christopher A. Karlsson, Maria Lutgring, Joseph D. Front Microbiol Microbiology Enterococcus faecalis and faecium with resistance to daptomycin and/or linezolid are emerging globally. We present the genomic characterization of daptomycin- and linezolid-resistant E. faecalis and E. faecium surveillance isolates from the United States, 2013–2016. Daptomycin resistance was low among E. faecalis (2/364, 0.5%) and E. faecium (17/344, 5%). The majority (71%, 12/17) of daptomycin-resistant E. faecium isolates belonged to the emerging ST736 clone and contained mutations in liaFSR and cls previously associated with resistance. However, 1/2 E. faecalis and 3/17 E. faecium did not contain these mutations previously associated with daptomycin resistance. Linezolid resistance was rare among E. faecalis (1/364, 0.3%) and E. faecium (2/344, 0.6%). These two E. faecium isolates, one of which was also resistant to daptomycin and vancomycin, contained the 23S rRNA nucleotide mutation (G2576T) associated with linezolid resistance. Long-read sequencing revealed the linezolid-resistant E. faecalis isolate contained chromosomal- and plasmid-encoded copies of optrA. The chromosomal optrA was located on the recently described Tn6674 multiresistance transposon. The second copy of optrA was encoded on an ∼65 kb mosaic plasmid, with component regions sharing high sequence identity to optrA-encoding multiresistance plasmids of animal origin. The optrA-encoding plasmid contained open reading frames predicted to encode proteins associated with a pheromone-responsive plasmid transfer system, and filter mating experiments confirmed the plasmid was conjugative. Continued surveillance of enterococci is necessary to assess the prevalence and trends of daptomycin and linezolid resistance in the United States, characterize resistance mechanisms and how they transfer, and monitor for emerging sequence types associated with resistance. Frontiers Media S.A. 2022-02-01 /pmc/articles/PMC8846945/ /pubmed/35178041 http://dx.doi.org/10.3389/fmicb.2021.807398 Text en Copyright © 2022 Gargis, Spicer, Kent, Zhu, Campbell, McAllister, Ewing, Albrecht, Stevens, Sheth, Padilla, Batra, Johnson, Halpin, Rasheed, Elkins, Karlsson and Lutgring. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gargis, Amy S.
Spicer, Lori M.
Kent, Alyssa G.
Zhu, Wenming
Campbell, Davina
McAllister, Gillian
Ewing, Thomas O.
Albrecht, Valerie
Stevens, Valerie A.
Sheth, Mili
Padilla, Jasmine
Batra, Dhwani
Johnson, J. Kristie
Halpin, Alison Laufer
Rasheed, J. Kamile
Elkins, Christopher A.
Karlsson, Maria
Lutgring, Joseph D.
Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title_full Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title_fullStr Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title_full_unstemmed Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title_short Sentinel Surveillance Reveals Emerging Daptomycin-Resistant ST736 Enterococcus faecium and Multiple Mechanisms of Linezolid Resistance in Enterococci in the United States
title_sort sentinel surveillance reveals emerging daptomycin-resistant st736 enterococcus faecium and multiple mechanisms of linezolid resistance in enterococci in the united states
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846945/
https://www.ncbi.nlm.nih.gov/pubmed/35178041
http://dx.doi.org/10.3389/fmicb.2021.807398
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