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The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats

In this study, we aim to investigate the effective dose of botulinum neurotoxin A that results in paralysis of the sternocleidomastoid muscle for a minimum duration of 28 days in Wistar rats. This research is the first in a series of studies to investigate the value of botulinum toxin A in the heali...

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Autores principales: Vampertzis, Themistoklis, Barmpagianni, Christina, Mpekiari, Chrysa, Baka, Rania, Zervos, Ioannis, Tsiridis, Eleftherios, Galanis, Nikiforos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846976/
https://www.ncbi.nlm.nih.gov/pubmed/35177957
http://dx.doi.org/10.1155/2022/2188783
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author Vampertzis, Themistoklis
Barmpagianni, Christina
Mpekiari, Chrysa
Baka, Rania
Zervos, Ioannis
Tsiridis, Eleftherios
Galanis, Nikiforos
author_facet Vampertzis, Themistoklis
Barmpagianni, Christina
Mpekiari, Chrysa
Baka, Rania
Zervos, Ioannis
Tsiridis, Eleftherios
Galanis, Nikiforos
author_sort Vampertzis, Themistoklis
collection PubMed
description In this study, we aim to investigate the effective dose of botulinum neurotoxin A that results in paralysis of the sternocleidomastoid muscle for a minimum duration of 28 days in Wistar rats. This research is the first in a series of studies to investigate the value of botulinum toxin A in the healing of clavicle fractures through the temporary paralysis of the sternocleidomastoid. A surgical incision was made under general anaesthesia, and botulinum neurotoxin A in respective doses of 4 and 6 international units (IU) or normal saline in equivalent volumes were injected directly into the exposed muscle. Electromyography was conducted on days 0, 7, and 28 following substance administration to determine the extent of muscle paralysis. Electromyography on day 0 showed no paralysis in either group. Animals injected with neurotoxin all exhibited paralysis on days 7 and 28 that was weaker in the group injected with the smaller dose of 4 IU. One death occurred in the group injected with the higher dose (6 IU), whereas in the control group, no paralysis was seen. Botulinum neurotoxin A in a dose of 6 IU resulted in complete paralysis of the sternocleidomastoid in rats for a minimum of 28 days. A dose of 4 IU resulted in less potent paralysis but was safer in our research. Botulinum neurotoxin is a substance utilised in cosmetics and therapeutics for many years, yet research shows that its use can be expanded to target a wider range of pathologies. In this series of studies, we aim to explore the neurotoxin's applications on the treatment of clavicle fractures. To investigate this, we need to first establish the duration of its action on the sternocleidomastoid muscle.
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spelling pubmed-88469762022-02-16 The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats Vampertzis, Themistoklis Barmpagianni, Christina Mpekiari, Chrysa Baka, Rania Zervos, Ioannis Tsiridis, Eleftherios Galanis, Nikiforos ScientificWorldJournal Research Article In this study, we aim to investigate the effective dose of botulinum neurotoxin A that results in paralysis of the sternocleidomastoid muscle for a minimum duration of 28 days in Wistar rats. This research is the first in a series of studies to investigate the value of botulinum toxin A in the healing of clavicle fractures through the temporary paralysis of the sternocleidomastoid. A surgical incision was made under general anaesthesia, and botulinum neurotoxin A in respective doses of 4 and 6 international units (IU) or normal saline in equivalent volumes were injected directly into the exposed muscle. Electromyography was conducted on days 0, 7, and 28 following substance administration to determine the extent of muscle paralysis. Electromyography on day 0 showed no paralysis in either group. Animals injected with neurotoxin all exhibited paralysis on days 7 and 28 that was weaker in the group injected with the smaller dose of 4 IU. One death occurred in the group injected with the higher dose (6 IU), whereas in the control group, no paralysis was seen. Botulinum neurotoxin A in a dose of 6 IU resulted in complete paralysis of the sternocleidomastoid in rats for a minimum of 28 days. A dose of 4 IU resulted in less potent paralysis but was safer in our research. Botulinum neurotoxin is a substance utilised in cosmetics and therapeutics for many years, yet research shows that its use can be expanded to target a wider range of pathologies. In this series of studies, we aim to explore the neurotoxin's applications on the treatment of clavicle fractures. To investigate this, we need to first establish the duration of its action on the sternocleidomastoid muscle. Hindawi 2022-02-08 /pmc/articles/PMC8846976/ /pubmed/35177957 http://dx.doi.org/10.1155/2022/2188783 Text en Copyright © 2022 Themistoklis Vampertzis et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vampertzis, Themistoklis
Barmpagianni, Christina
Mpekiari, Chrysa
Baka, Rania
Zervos, Ioannis
Tsiridis, Eleftherios
Galanis, Nikiforos
The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title_full The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title_fullStr The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title_full_unstemmed The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title_short The Action of Botulinum Toxin A on the Sternocleidomastoid Muscle: An Experimental Study on Rats
title_sort action of botulinum toxin a on the sternocleidomastoid muscle: an experimental study on rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846976/
https://www.ncbi.nlm.nih.gov/pubmed/35177957
http://dx.doi.org/10.1155/2022/2188783
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