Cargando…

N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma

N6-methyladenosine (m6A) and long non-coding RNAs (lncRNAs) play vital roles in the prognostic value and immune microenvironment of malignant tumors. Here, we constructed a m6A-related lncRNA signature in osteosarcoma samples from TCGA dataset and analyzed the association of the signature with tumor...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Di, Yu, Ling, Wei, Zhun, Xia, Kezhou, Guo, Weichun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847230/
https://www.ncbi.nlm.nih.gov/pubmed/35186011
http://dx.doi.org/10.3389/fgene.2021.805607
_version_ 1784652009124659200
author Zheng, Di
Yu, Ling
Wei, Zhun
Xia, Kezhou
Guo, Weichun
author_facet Zheng, Di
Yu, Ling
Wei, Zhun
Xia, Kezhou
Guo, Weichun
author_sort Zheng, Di
collection PubMed
description N6-methyladenosine (m6A) and long non-coding RNAs (lncRNAs) play vital roles in the prognostic value and immune microenvironment of malignant tumors. Here, we constructed a m6A-related lncRNA signature in osteosarcoma samples from TCGA dataset and analyzed the association of the signature with tumor immune microenvironment. m6A-related lncRNAs were identified by performing Pearson’s correlation analysis and were used to construct a novel m6A-related lncRNA signature in osteosarcoma. Validation in testing and entire cohorts confirmed the satisfactory accuracy of the risk signature. Principal-component analysis verifies the grouping ability of the risk signature. Functional enrichment analyses connected immune with the risk signature based on the six m6A-related lncRNAs. When patients were separated into high- and low-risk group based on their risk scores, we found that patients in the high-risk group had lower stromal scores, immune scores, and ESTIMATE scores, while the tumor purity was higher in the high-risk group than that in the low-risk group. As for immune cell infiltration, the proportion of monocytes was significantly higher in the low-risk group than that in the high-risk group. Of the six lncRNAs, AC004812.2 was a protective factor in osteosarcoma and low expression of AC004812.2 predicted worse overall survival. Overexpression of AC004812.2 inhibited 143B cell proliferation and increased the expression levels of IGF2BP1 and YTHDF1. In all, our m6A-related lncRNA signature was a potential prognostic biomarker and correlated with tumor immune microenvironment and immune cell infiltration, and AC004812.2 might be an important regulator of m6A modification and a promising therapeutic target in osteosarcoma.
format Online
Article
Text
id pubmed-8847230
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88472302022-02-17 N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma Zheng, Di Yu, Ling Wei, Zhun Xia, Kezhou Guo, Weichun Front Genet Genetics N6-methyladenosine (m6A) and long non-coding RNAs (lncRNAs) play vital roles in the prognostic value and immune microenvironment of malignant tumors. Here, we constructed a m6A-related lncRNA signature in osteosarcoma samples from TCGA dataset and analyzed the association of the signature with tumor immune microenvironment. m6A-related lncRNAs were identified by performing Pearson’s correlation analysis and were used to construct a novel m6A-related lncRNA signature in osteosarcoma. Validation in testing and entire cohorts confirmed the satisfactory accuracy of the risk signature. Principal-component analysis verifies the grouping ability of the risk signature. Functional enrichment analyses connected immune with the risk signature based on the six m6A-related lncRNAs. When patients were separated into high- and low-risk group based on their risk scores, we found that patients in the high-risk group had lower stromal scores, immune scores, and ESTIMATE scores, while the tumor purity was higher in the high-risk group than that in the low-risk group. As for immune cell infiltration, the proportion of monocytes was significantly higher in the low-risk group than that in the high-risk group. Of the six lncRNAs, AC004812.2 was a protective factor in osteosarcoma and low expression of AC004812.2 predicted worse overall survival. Overexpression of AC004812.2 inhibited 143B cell proliferation and increased the expression levels of IGF2BP1 and YTHDF1. In all, our m6A-related lncRNA signature was a potential prognostic biomarker and correlated with tumor immune microenvironment and immune cell infiltration, and AC004812.2 might be an important regulator of m6A modification and a promising therapeutic target in osteosarcoma. Frontiers Media S.A. 2022-02-02 /pmc/articles/PMC8847230/ /pubmed/35186011 http://dx.doi.org/10.3389/fgene.2021.805607 Text en Copyright © 2022 Zheng, Yu, Wei, Xia and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zheng, Di
Yu, Ling
Wei, Zhun
Xia, Kezhou
Guo, Weichun
N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title_full N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title_fullStr N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title_full_unstemmed N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title_short N6-Methyladenosine-Related lncRNAs Are Potential Prognostic Biomarkers and Correlated With Tumor Immune Microenvironment in Osteosarcoma
title_sort n6-methyladenosine-related lncrnas are potential prognostic biomarkers and correlated with tumor immune microenvironment in osteosarcoma
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847230/
https://www.ncbi.nlm.nih.gov/pubmed/35186011
http://dx.doi.org/10.3389/fgene.2021.805607
work_keys_str_mv AT zhengdi n6methyladenosinerelatedlncrnasarepotentialprognosticbiomarkersandcorrelatedwithtumorimmunemicroenvironmentinosteosarcoma
AT yuling n6methyladenosinerelatedlncrnasarepotentialprognosticbiomarkersandcorrelatedwithtumorimmunemicroenvironmentinosteosarcoma
AT weizhun n6methyladenosinerelatedlncrnasarepotentialprognosticbiomarkersandcorrelatedwithtumorimmunemicroenvironmentinosteosarcoma
AT xiakezhou n6methyladenosinerelatedlncrnasarepotentialprognosticbiomarkersandcorrelatedwithtumorimmunemicroenvironmentinosteosarcoma
AT guoweichun n6methyladenosinerelatedlncrnasarepotentialprognosticbiomarkersandcorrelatedwithtumorimmunemicroenvironmentinosteosarcoma