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The effects of temperature variation treatments on embryonic development: a mouse study

Since the development of ART, embryos have been cultured at 37 °C in an attempt to mimic the in vivo conditions and the average body temperature of an adult. However, a gradient of temperatures within the reproductive tract has been demonstrated in humans and several other mammalian species. Therefo...

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Autores principales: Moriyama, Dóris Ferreira, Makri, Dimitra, Maalouf, Mary-Naya, Adamova, Petra, de Moraes, Gabrielle Ferrante Alves, Pinheiro, Marcela de Oliveira, Bernardineli, Danilo Lessa, Massaia, Irineu Francisco Delfino Silva, Maalouf, Walid E., Lo Turco, Edson Guimarães
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847426/
https://www.ncbi.nlm.nih.gov/pubmed/35169175
http://dx.doi.org/10.1038/s41598-022-06158-y
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author Moriyama, Dóris Ferreira
Makri, Dimitra
Maalouf, Mary-Naya
Adamova, Petra
de Moraes, Gabrielle Ferrante Alves
Pinheiro, Marcela de Oliveira
Bernardineli, Danilo Lessa
Massaia, Irineu Francisco Delfino Silva
Maalouf, Walid E.
Lo Turco, Edson Guimarães
author_facet Moriyama, Dóris Ferreira
Makri, Dimitra
Maalouf, Mary-Naya
Adamova, Petra
de Moraes, Gabrielle Ferrante Alves
Pinheiro, Marcela de Oliveira
Bernardineli, Danilo Lessa
Massaia, Irineu Francisco Delfino Silva
Maalouf, Walid E.
Lo Turco, Edson Guimarães
author_sort Moriyama, Dóris Ferreira
collection PubMed
description Since the development of ART, embryos have been cultured at 37 °C in an attempt to mimic the in vivo conditions and the average body temperature of an adult. However, a gradient of temperatures within the reproductive tract has been demonstrated in humans and several other mammalian species. Therefore, the aim of this study was to evaluate the effects of temperature variation treatments on mouse embryo quality through morphokinetic events, blastocyst morphology, the relative gene expression of Igf2, Bax, Bcl2 and Apaf1 and the metabolomics of individual culture media. Study groups consisted of 2 circadian treatments, T1 with embryos being cultured at 37 °C during the day and 35.5 °C during the night, T2 with 38.5 °C during the day and 37 °C during the night and a control group with constant 37 °C. Our main findings are that the lower-temperature group (T1) showed a consistent negative effect on mouse embryo development with “slow” cleaving embryos, poor-quality blastocysts, a higher expression of the apoptotic gene Apaf1, and a significantly different set of amino acids representing a more stressed metabolism. On the other hand, our higher-temperature group (T2) showed similar results to the control group, with no adverse effects on blastocyst viability.
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spelling pubmed-88474262022-02-17 The effects of temperature variation treatments on embryonic development: a mouse study Moriyama, Dóris Ferreira Makri, Dimitra Maalouf, Mary-Naya Adamova, Petra de Moraes, Gabrielle Ferrante Alves Pinheiro, Marcela de Oliveira Bernardineli, Danilo Lessa Massaia, Irineu Francisco Delfino Silva Maalouf, Walid E. Lo Turco, Edson Guimarães Sci Rep Article Since the development of ART, embryos have been cultured at 37 °C in an attempt to mimic the in vivo conditions and the average body temperature of an adult. However, a gradient of temperatures within the reproductive tract has been demonstrated in humans and several other mammalian species. Therefore, the aim of this study was to evaluate the effects of temperature variation treatments on mouse embryo quality through morphokinetic events, blastocyst morphology, the relative gene expression of Igf2, Bax, Bcl2 and Apaf1 and the metabolomics of individual culture media. Study groups consisted of 2 circadian treatments, T1 with embryos being cultured at 37 °C during the day and 35.5 °C during the night, T2 with 38.5 °C during the day and 37 °C during the night and a control group with constant 37 °C. Our main findings are that the lower-temperature group (T1) showed a consistent negative effect on mouse embryo development with “slow” cleaving embryos, poor-quality blastocysts, a higher expression of the apoptotic gene Apaf1, and a significantly different set of amino acids representing a more stressed metabolism. On the other hand, our higher-temperature group (T2) showed similar results to the control group, with no adverse effects on blastocyst viability. Nature Publishing Group UK 2022-02-15 /pmc/articles/PMC8847426/ /pubmed/35169175 http://dx.doi.org/10.1038/s41598-022-06158-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Moriyama, Dóris Ferreira
Makri, Dimitra
Maalouf, Mary-Naya
Adamova, Petra
de Moraes, Gabrielle Ferrante Alves
Pinheiro, Marcela de Oliveira
Bernardineli, Danilo Lessa
Massaia, Irineu Francisco Delfino Silva
Maalouf, Walid E.
Lo Turco, Edson Guimarães
The effects of temperature variation treatments on embryonic development: a mouse study
title The effects of temperature variation treatments on embryonic development: a mouse study
title_full The effects of temperature variation treatments on embryonic development: a mouse study
title_fullStr The effects of temperature variation treatments on embryonic development: a mouse study
title_full_unstemmed The effects of temperature variation treatments on embryonic development: a mouse study
title_short The effects of temperature variation treatments on embryonic development: a mouse study
title_sort effects of temperature variation treatments on embryonic development: a mouse study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847426/
https://www.ncbi.nlm.nih.gov/pubmed/35169175
http://dx.doi.org/10.1038/s41598-022-06158-y
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