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Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46

The quest for novel broad spectrum bioactive compounds is needed continuously because of the rapid advent of pathogenic multi drug resistant organisms. Actinomycetes, isolated from unexplored habitats can be a solution of this problem. The motive of this research work was isolation of actinomycetes...

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Autores principales: Chakraborty, Bidhayak, Kumar, Raju Suresh, Almansour, Abdulrahman I., Gunasekaran, Pethaiah, Nayaka, Sreenivasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847965/
https://www.ncbi.nlm.nih.gov/pubmed/35197732
http://dx.doi.org/10.1016/j.sjbs.2021.11.055
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author Chakraborty, Bidhayak
Kumar, Raju Suresh
Almansour, Abdulrahman I.
Gunasekaran, Pethaiah
Nayaka, Sreenivasa
author_facet Chakraborty, Bidhayak
Kumar, Raju Suresh
Almansour, Abdulrahman I.
Gunasekaran, Pethaiah
Nayaka, Sreenivasa
author_sort Chakraborty, Bidhayak
collection PubMed
description The quest for novel broad spectrum bioactive compounds is needed continuously because of the rapid advent of pathogenic multi drug resistant organisms. Actinomycetes, isolated from unexplored habitats can be a solution of this problem. The motive of this research work was isolation of actinomycetes having potential antimicrobial activities from unexplored regions of Devbag and Tilmati beach. The isolated actinomycetes were screened against pathogenic microbes for antimicrobial activities through cross streak method. Enzyme production activity was checked for these actinomycetes for amylase, protease, cellulase and lipase enzymes. Further antimicrobial activity of ethyl acetate extract of the potent strain KS46 was performed. The strain KS46 was identified with 16S rRNA gene sequencing and secondary structure was analysed. Gas chromatography–Mass spectrometry (GC–MS) profiling was conducted to ascertain the presence of bioactive metabolites in the ethyl acetate extract. The collected samples were pre-treated and 70 actinomycetes were isolated. The Streptomyces sp. strain KS46 showed the best antimicrobial activity in primary screening. Ethyl acetate extract of the strain KS46 revealed antimicrobial activity against S. aureus, B. subtilis, B. cereus, E. faecalis, K. pneumoniae, E. coli, S. flexneri, C. albicans and C. glabrata. The 16S rRNA gene sequencing identified the strain KS46 as Streptomyces levis strain KS46. The GC–MS metabolite profiling of the ethyl acetate extract revealed the availability of 42 compounds including fatty acid esters, fatty acid anhydrides, alkanes, steroids, esters, alcohols, carboxylic ester, etc. having antibacterial, antifungal, antiproliferative, antioxidant activities. This study indicated that Devbag and Tilmati beaches being untapped habitats have enormous diversity of promising antimicrobial metabolite producing actinomycetes. Therefore, further exploration should be carried out to characterize the potential actinomycetes, which can be optimistic candidates for generation of novel antimicrobial drugs.
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spelling pubmed-88479652022-02-22 Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46 Chakraborty, Bidhayak Kumar, Raju Suresh Almansour, Abdulrahman I. Gunasekaran, Pethaiah Nayaka, Sreenivasa Saudi J Biol Sci Original Article The quest for novel broad spectrum bioactive compounds is needed continuously because of the rapid advent of pathogenic multi drug resistant organisms. Actinomycetes, isolated from unexplored habitats can be a solution of this problem. The motive of this research work was isolation of actinomycetes having potential antimicrobial activities from unexplored regions of Devbag and Tilmati beach. The isolated actinomycetes were screened against pathogenic microbes for antimicrobial activities through cross streak method. Enzyme production activity was checked for these actinomycetes for amylase, protease, cellulase and lipase enzymes. Further antimicrobial activity of ethyl acetate extract of the potent strain KS46 was performed. The strain KS46 was identified with 16S rRNA gene sequencing and secondary structure was analysed. Gas chromatography–Mass spectrometry (GC–MS) profiling was conducted to ascertain the presence of bioactive metabolites in the ethyl acetate extract. The collected samples were pre-treated and 70 actinomycetes were isolated. The Streptomyces sp. strain KS46 showed the best antimicrobial activity in primary screening. Ethyl acetate extract of the strain KS46 revealed antimicrobial activity against S. aureus, B. subtilis, B. cereus, E. faecalis, K. pneumoniae, E. coli, S. flexneri, C. albicans and C. glabrata. The 16S rRNA gene sequencing identified the strain KS46 as Streptomyces levis strain KS46. The GC–MS metabolite profiling of the ethyl acetate extract revealed the availability of 42 compounds including fatty acid esters, fatty acid anhydrides, alkanes, steroids, esters, alcohols, carboxylic ester, etc. having antibacterial, antifungal, antiproliferative, antioxidant activities. This study indicated that Devbag and Tilmati beaches being untapped habitats have enormous diversity of promising antimicrobial metabolite producing actinomycetes. Therefore, further exploration should be carried out to characterize the potential actinomycetes, which can be optimistic candidates for generation of novel antimicrobial drugs. Elsevier 2022-02 2021-12-01 /pmc/articles/PMC8847965/ /pubmed/35197732 http://dx.doi.org/10.1016/j.sjbs.2021.11.055 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Chakraborty, Bidhayak
Kumar, Raju Suresh
Almansour, Abdulrahman I.
Gunasekaran, Pethaiah
Nayaka, Sreenivasa
Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title_full Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title_fullStr Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title_full_unstemmed Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title_short Bioprospection and secondary metabolites profiling of marine Streptomyces levis strain KS46
title_sort bioprospection and secondary metabolites profiling of marine streptomyces levis strain ks46
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8847965/
https://www.ncbi.nlm.nih.gov/pubmed/35197732
http://dx.doi.org/10.1016/j.sjbs.2021.11.055
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