The pioneer use of a modified PRGF–Endoret(®) technique for wound healing in a hemodialyzed diabetic patient in a terminal stage of renal disease

In the literature, this paper is the first to describe the use of plasma rich in growth factors (PRGF)–Endoret(®) in hemodialyzed diabetic patients, to promote the healing of after amputation wounds. The PRGF–Endoret(®) was primarily conceived to be used in maxillofacial surgery, oral implantology, etc., the innovation residing in the blood collection technique (quantity, moment of the week, rhythmicity), which was adapted to the specific conditions of the hemodialyzed patient. Moreover, in the initial phases, the two PRGF fractions were innovatively applied as single alternating layers on the wound surface. Only after the surface of the wound decreased, the two PRGF fractions were applied as overlapping layers. Nevertheless, the paper presents the optimal method to assess the clinical evolution of the wound. Histopathological examination of the biopsy performed during wound preparation for PRGF application brought additional, essential data for orienting the therapeutic approach. The exclusion of calciphylaxis, a disease with high mortality risk, encouraged the application of this method, and also demonstrated the microscopic features in hemodialyzed diabetic patients.