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Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models
A two-dimensional (2D) cell culture-based model is widely applied to study tumorigenic mechanisms and drug screening. However, it cannot authentically simulate the three-dimensional (3D) microenvironment of solid tumors and provide reliable and predictable data in response to in vivo, thus leading t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848337/ https://www.ncbi.nlm.nih.gov/pubmed/35233536 http://dx.doi.org/10.34133/2022/9809763 |
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author | He, Jiachen Chen, Chichi Chen, Liang Cheng, Ruoyu Sun, Jie Liu, Xingzhi Wang, Lin Zhu, Can Hu, Sihan Xue, Yuan Lu, Jian Yang, Huiling Cui, Wenguo Shi, Qin |
author_facet | He, Jiachen Chen, Chichi Chen, Liang Cheng, Ruoyu Sun, Jie Liu, Xingzhi Wang, Lin Zhu, Can Hu, Sihan Xue, Yuan Lu, Jian Yang, Huiling Cui, Wenguo Shi, Qin |
author_sort | He, Jiachen |
collection | PubMed |
description | A two-dimensional (2D) cell culture-based model is widely applied to study tumorigenic mechanisms and drug screening. However, it cannot authentically simulate the three-dimensional (3D) microenvironment of solid tumors and provide reliable and predictable data in response to in vivo, thus leading to the research illusions and failure of drug screening. In this study, honeycomb-like gelatin methacryloyl (GelMA) hydrogel microspheres are developed by synchronous photocrosslinking microfluidic technique to construct a 3D model of osteosarcoma. The in vitro study shows that osteosarcoma cells (K7M2) cultured in 3D GelMA microspheres have stronger tumorous stemness, proliferation and migration abilities, more osteoclastogenetic ability, and resistance to chemotherapeutic drugs (DOX) than that of cells in 2D cultures. More importantly, the 3D-cultured K7M2 cells show more tumorigenicity in immunologically sound mice, characterized by shorter tumorigenesis time, larger tumor volume, severe bone destruction, and higher mortality. In conclusion, honeycomb-like porous microsphere scaffolds are constructed with uniform structure by microfluidic technology to massively produce tumor cells with original phenotypes. Those microspheres could recapitulate the physiology microenvironment of tumors, maintain cell-cell and cell-extracellular matrix interactions, and thus provide an effective and convenient strategy for tumor pathogenesis and drug screening research. |
format | Online Article Text |
id | pubmed-8848337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-88483372022-02-28 Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models He, Jiachen Chen, Chichi Chen, Liang Cheng, Ruoyu Sun, Jie Liu, Xingzhi Wang, Lin Zhu, Can Hu, Sihan Xue, Yuan Lu, Jian Yang, Huiling Cui, Wenguo Shi, Qin Research (Wash D C) Research Article A two-dimensional (2D) cell culture-based model is widely applied to study tumorigenic mechanisms and drug screening. However, it cannot authentically simulate the three-dimensional (3D) microenvironment of solid tumors and provide reliable and predictable data in response to in vivo, thus leading to the research illusions and failure of drug screening. In this study, honeycomb-like gelatin methacryloyl (GelMA) hydrogel microspheres are developed by synchronous photocrosslinking microfluidic technique to construct a 3D model of osteosarcoma. The in vitro study shows that osteosarcoma cells (K7M2) cultured in 3D GelMA microspheres have stronger tumorous stemness, proliferation and migration abilities, more osteoclastogenetic ability, and resistance to chemotherapeutic drugs (DOX) than that of cells in 2D cultures. More importantly, the 3D-cultured K7M2 cells show more tumorigenicity in immunologically sound mice, characterized by shorter tumorigenesis time, larger tumor volume, severe bone destruction, and higher mortality. In conclusion, honeycomb-like porous microsphere scaffolds are constructed with uniform structure by microfluidic technology to massively produce tumor cells with original phenotypes. Those microspheres could recapitulate the physiology microenvironment of tumors, maintain cell-cell and cell-extracellular matrix interactions, and thus provide an effective and convenient strategy for tumor pathogenesis and drug screening research. AAAS 2022-02-07 /pmc/articles/PMC8848337/ /pubmed/35233536 http://dx.doi.org/10.34133/2022/9809763 Text en Copyright © 2022 Jiachen He et al. https://creativecommons.org/licenses/by/4.0/Exclusive Licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0). |
spellingShingle | Research Article He, Jiachen Chen, Chichi Chen, Liang Cheng, Ruoyu Sun, Jie Liu, Xingzhi Wang, Lin Zhu, Can Hu, Sihan Xue, Yuan Lu, Jian Yang, Huiling Cui, Wenguo Shi, Qin Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title_full | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title_fullStr | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title_full_unstemmed | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title_short | Honeycomb-Like Hydrogel Microspheres for 3D Bulk Construction of Tumor Models |
title_sort | honeycomb-like hydrogel microspheres for 3d bulk construction of tumor models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848337/ https://www.ncbi.nlm.nih.gov/pubmed/35233536 http://dx.doi.org/10.34133/2022/9809763 |
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