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MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury
miR-101a-3p is expressed in a variety of organs and tissues and plays a regulatory role in many diseases, but its role in spinal cord ischemia/reperfusion injury remains unclear. In this study, we established a rat model of spinal cord ischemia/reperfusion injury by clamping the aortic arch for 14 m...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848611/ https://www.ncbi.nlm.nih.gov/pubmed/35142692 http://dx.doi.org/10.4103/1673-5374.335164 |
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author | Zhang, Zai-Li Wang, Dan Chen, Feng-Shou |
author_facet | Zhang, Zai-Li Wang, Dan Chen, Feng-Shou |
author_sort | Zhang, Zai-Li |
collection | PubMed |
description | miR-101a-3p is expressed in a variety of organs and tissues and plays a regulatory role in many diseases, but its role in spinal cord ischemia/reperfusion injury remains unclear. In this study, we established a rat model of spinal cord ischemia/reperfusion injury by clamping the aortic arch for 14 minutes followed by reperfusion for 24 hours. Results showed that miR-101a-3p expression in L4–L6 spinal cord was greatly decreased, whereas MYCN expression was greatly increased. Dual-luciferase reporter assay results showed that miR-101a-3p targeted MYCN. MYCN immunoreactivity, which was primarily colocalized with neurons in L4–L6 spinal tissue, greatly increased after spinal cord ischemia/reperfusion injury. However, intrathecal injection of an miR-101a-3p mimic within 24 hours before injury decreased MYCN, p53, caspase-9 and interleukin-1β expression, reduced p53 immunoreactivity, reduced the number of MYCN/NeuN-positive cells and the number of necrotic cells in L4–L6 spinal tissue, and increased Tarlov scores. These findings suggest that the miR-101a-3p mimic improved spinal ischemia/reperfusion injury-induced nerve cell apoptosis and inflammation by inhibiting MYCN and the p53 signaling pathway. Therefore, miR-101a-3p mimic therapy may be a potential treatment option for spinal ischemia/reperfusion injury. |
format | Online Article Text |
id | pubmed-8848611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-88486112022-03-08 MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury Zhang, Zai-Li Wang, Dan Chen, Feng-Shou Neural Regen Res Research Article miR-101a-3p is expressed in a variety of organs and tissues and plays a regulatory role in many diseases, but its role in spinal cord ischemia/reperfusion injury remains unclear. In this study, we established a rat model of spinal cord ischemia/reperfusion injury by clamping the aortic arch for 14 minutes followed by reperfusion for 24 hours. Results showed that miR-101a-3p expression in L4–L6 spinal cord was greatly decreased, whereas MYCN expression was greatly increased. Dual-luciferase reporter assay results showed that miR-101a-3p targeted MYCN. MYCN immunoreactivity, which was primarily colocalized with neurons in L4–L6 spinal tissue, greatly increased after spinal cord ischemia/reperfusion injury. However, intrathecal injection of an miR-101a-3p mimic within 24 hours before injury decreased MYCN, p53, caspase-9 and interleukin-1β expression, reduced p53 immunoreactivity, reduced the number of MYCN/NeuN-positive cells and the number of necrotic cells in L4–L6 spinal tissue, and increased Tarlov scores. These findings suggest that the miR-101a-3p mimic improved spinal ischemia/reperfusion injury-induced nerve cell apoptosis and inflammation by inhibiting MYCN and the p53 signaling pathway. Therefore, miR-101a-3p mimic therapy may be a potential treatment option for spinal ischemia/reperfusion injury. Wolters Kluwer - Medknow 2022-02-08 /pmc/articles/PMC8848611/ /pubmed/35142692 http://dx.doi.org/10.4103/1673-5374.335164 Text en Copyright: © Neural Regeneration Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Zhang, Zai-Li Wang, Dan Chen, Feng-Shou MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title | MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title_full | MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title_fullStr | MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title_full_unstemmed | MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title_short | MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
title_sort | microrna-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848611/ https://www.ncbi.nlm.nih.gov/pubmed/35142692 http://dx.doi.org/10.4103/1673-5374.335164 |
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