Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immunostaining

Detection of nucleic acids within subcellular compartments is key to understanding their function. Determining the intracellular distribution of nucleic acids requires quantitative retention and estimation of their association with different organelles by immunofluorescence microscopy. This is parti...

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Detalles Bibliográficos
Autores principales: Paramasivam, Prasath, Stöter, Martin, Corradi, Eloina, Dalla Costa, Irene, Höijer, Andreas, Bartesaghi, Stefano, Sabirsh, Alan, Lindfors, Lennart, Yanez Arteta, Marianna, Nordberg, Peter, Andersson, Shalini, Baudet, Marie-Laure, Bickle, Marc, Zerial, Marino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2022
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848937/
https://www.ncbi.nlm.nih.gov/pubmed/34949721
http://dx.doi.org/10.1261/rna.078895.121

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