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Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes
BACKGROUND: This study aimed to analyze the in vitro effect of micro-strain stress on the proliferation and functional marker expression in chondrocytes isolated from human osteoarthritis cartilage samples. METHODS: Chondrocytes isolated from human osteoarthritis cartilage samples were subjected to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848938/ https://www.ncbi.nlm.nih.gov/pubmed/35168651 http://dx.doi.org/10.1186/s13018-022-02987-9 |
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author | Zhao, Bin Ma, Jianxiong He, Jinquan Ma, Xinlong |
author_facet | Zhao, Bin Ma, Jianxiong He, Jinquan Ma, Xinlong |
author_sort | Zhao, Bin |
collection | PubMed |
description | BACKGROUND: This study aimed to analyze the in vitro effect of micro-strain stress on the proliferation and functional marker expression in chondrocytes isolated from human osteoarthritis cartilage samples. METHODS: Chondrocytes isolated from human osteoarthritis cartilage samples were subjected to loading with different types of micro-strain stress. The proliferation activity was assessed by flow cytometry, and the functional expression of chondrocyte markers was detected by qRT-PCR and western blot. RESULTS: Flow cytometry results showed stimulation of proliferation of human osteoarthritic chondrocytes when an adequate micro-strain stress was applied. qRT-PCR and western blot results showed that micro-strain stress promotes human osteoarthritic chondrocyte functional marker expression. These features coincide with the upregulation of multiple proteins and genes affecting cell proliferation and functional chondrocyte marker expression, including cyclin D1, collagen II, and Rock. CONCLUSION: Adequate micro-strain stress could activate the Rho/Rock signaling pathway in osteoarthritic chondrocytes, thus transmitting mechanical signals to the cytoskeleton. This process leads to cytoskeleton reorganization, and transmission of the mechanical signals to the downstream effectors to promote proliferation and functional marker expression of osteoarthritic chondrocytes. |
format | Online Article Text |
id | pubmed-8848938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-88489382022-02-18 Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes Zhao, Bin Ma, Jianxiong He, Jinquan Ma, Xinlong J Orthop Surg Res Research Article BACKGROUND: This study aimed to analyze the in vitro effect of micro-strain stress on the proliferation and functional marker expression in chondrocytes isolated from human osteoarthritis cartilage samples. METHODS: Chondrocytes isolated from human osteoarthritis cartilage samples were subjected to loading with different types of micro-strain stress. The proliferation activity was assessed by flow cytometry, and the functional expression of chondrocyte markers was detected by qRT-PCR and western blot. RESULTS: Flow cytometry results showed stimulation of proliferation of human osteoarthritic chondrocytes when an adequate micro-strain stress was applied. qRT-PCR and western blot results showed that micro-strain stress promotes human osteoarthritic chondrocyte functional marker expression. These features coincide with the upregulation of multiple proteins and genes affecting cell proliferation and functional chondrocyte marker expression, including cyclin D1, collagen II, and Rock. CONCLUSION: Adequate micro-strain stress could activate the Rho/Rock signaling pathway in osteoarthritic chondrocytes, thus transmitting mechanical signals to the cytoskeleton. This process leads to cytoskeleton reorganization, and transmission of the mechanical signals to the downstream effectors to promote proliferation and functional marker expression of osteoarthritic chondrocytes. BioMed Central 2022-02-15 /pmc/articles/PMC8848938/ /pubmed/35168651 http://dx.doi.org/10.1186/s13018-022-02987-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zhao, Bin Ma, Jianxiong He, Jinquan Ma, Xinlong Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title | Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title_full | Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title_fullStr | Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title_full_unstemmed | Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title_short | Effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
title_sort | effect of micro-strain stress on in vitro proliferation and functional expression of human osteoarthritic chondrocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8848938/ https://www.ncbi.nlm.nih.gov/pubmed/35168651 http://dx.doi.org/10.1186/s13018-022-02987-9 |
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