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Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus
Severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus are endemic zoonotic diseases that pose significant public health threats in East Asia. As these two diseases share common clinical features, as well as overlapping disease regions, it is difficult to differentiate between SFTS and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8849512/ https://www.ncbi.nlm.nih.gov/pubmed/35171943 http://dx.doi.org/10.1371/journal.pone.0262302 |
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author | Jang, Woong Sik Lim, Da Hye Choe, Young Lan Nam, Jeonghun Moon, Kyung Chul Kim, Chaewon Choi, Minkyeong Park, Insu Park, Dae Won Lim, Chae Seung |
author_facet | Jang, Woong Sik Lim, Da Hye Choe, Young Lan Nam, Jeonghun Moon, Kyung Chul Kim, Chaewon Choi, Minkyeong Park, Insu Park, Dae Won Lim, Chae Seung |
author_sort | Jang, Woong Sik |
collection | PubMed |
description | Severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus are endemic zoonotic diseases that pose significant public health threats in East Asia. As these two diseases share common clinical features, as well as overlapping disease regions, it is difficult to differentiate between SFTS and scrub typhus. A multiplex reverse-transcription loop‑mediated isothermal amplification (RT-LAMP) assay was developed to detect large segments and GroES genes for SFTS virus (SFTSV) and Orientia tsutsugamushi (OT). The performance of the RT-LAMP assay was compared and evaluated with those of commercial PowerChek(™) SFTSV real-time PCR and LiliF(™) TSUTSU nested PCR for 23 SFTS and 12 scrub typhus clinical samples, respectively. The multiplex SFTSV/OT/Internal control (IC) RT-LAMP assay showed comparable sensitivity (91.3%) with that of commercial PowerChek(™) SFTSV Real-time PCR (95.6%) and higher sensitivity (91.6%) than that of LiliF(™) TSUTSU nested PCR (75%). In addition, the multiplex SFTSV/OT RT-LAMP assay showed 100% specificity and no cross-reactivity for blood from uninfected healthy patients and samples from patients infected with other fever viruses. Thus, the multiplex SFTSV/OT/IC RT-LAMP assay could serve as a useful point-of-care molecular diagnostic test for SFTS and scrub typhus. |
format | Online Article Text |
id | pubmed-8849512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-88495122022-02-17 Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus Jang, Woong Sik Lim, Da Hye Choe, Young Lan Nam, Jeonghun Moon, Kyung Chul Kim, Chaewon Choi, Minkyeong Park, Insu Park, Dae Won Lim, Chae Seung PLoS One Research Article Severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus are endemic zoonotic diseases that pose significant public health threats in East Asia. As these two diseases share common clinical features, as well as overlapping disease regions, it is difficult to differentiate between SFTS and scrub typhus. A multiplex reverse-transcription loop‑mediated isothermal amplification (RT-LAMP) assay was developed to detect large segments and GroES genes for SFTS virus (SFTSV) and Orientia tsutsugamushi (OT). The performance of the RT-LAMP assay was compared and evaluated with those of commercial PowerChek(™) SFTSV real-time PCR and LiliF(™) TSUTSU nested PCR for 23 SFTS and 12 scrub typhus clinical samples, respectively. The multiplex SFTSV/OT/Internal control (IC) RT-LAMP assay showed comparable sensitivity (91.3%) with that of commercial PowerChek(™) SFTSV Real-time PCR (95.6%) and higher sensitivity (91.6%) than that of LiliF(™) TSUTSU nested PCR (75%). In addition, the multiplex SFTSV/OT RT-LAMP assay showed 100% specificity and no cross-reactivity for blood from uninfected healthy patients and samples from patients infected with other fever viruses. Thus, the multiplex SFTSV/OT/IC RT-LAMP assay could serve as a useful point-of-care molecular diagnostic test for SFTS and scrub typhus. Public Library of Science 2022-02-16 /pmc/articles/PMC8849512/ /pubmed/35171943 http://dx.doi.org/10.1371/journal.pone.0262302 Text en © 2022 Jang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Jang, Woong Sik Lim, Da Hye Choe, Young Lan Nam, Jeonghun Moon, Kyung Chul Kim, Chaewon Choi, Minkyeong Park, Insu Park, Dae Won Lim, Chae Seung Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title | Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title_full | Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title_fullStr | Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title_full_unstemmed | Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title_short | Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus |
title_sort | developing a multiplex loop-mediated isothermal amplification assay (lamp) to determine severe fever with thrombocytopenia syndrome (sfts) and scrub typhus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8849512/ https://www.ncbi.nlm.nih.gov/pubmed/35171943 http://dx.doi.org/10.1371/journal.pone.0262302 |
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