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Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation

All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar...

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Autores principales: Grover, Gomti, Sharma, Achla, Mackay, Ian, Srivastava, Puja, Kaur, Satinder, Kaur, Jaspal, Burridge, Amanda, Allen, Sacha Przewieslik, Bentley, Alison R., Chhuneja, Parveen, Bains, N. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8849526/
https://www.ncbi.nlm.nih.gov/pubmed/35171951
http://dx.doi.org/10.1371/journal.pone.0264027
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author Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N. S.
author_facet Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N. S.
author_sort Grover, Gomti
collection PubMed
description All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar ‘HD 2967’. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from ‘Acienda’ has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance.
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spelling pubmed-88495262022-02-17 Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation Grover, Gomti Sharma, Achla Mackay, Ian Srivastava, Puja Kaur, Satinder Kaur, Jaspal Burridge, Amanda Allen, Sacha Przewieslik Bentley, Alison R. Chhuneja, Parveen Bains, N. S. PLoS One Research Article All stage resistance to stripe rust races prevalent in India was investigated in the European winter wheat cultivar ‘Acienda’. In order to dissect the genetic basis of the resistance, a backcross population was developed between ‘Acienda’ and the stripe rust susceptible Indian spring wheat cultivar ‘HD 2967’. Inheritance studies revealed segregation for a dominant resistant gene. High density SNP genotyping was used to map stripe rust resistance and marker regression analysis located stripe rust resistance to the distal end of wheat chromosome 1A. Interval mapping located this region between the SNP markers AX-95162217 and AX-94540853, at a LOD score of 15.83 with a phenotypic contribution of 60%. This major stripe rust resistance locus from ‘Acienda’ has been temporarily designated as Yraci. A candidate gene search in the 2.76 Mb region carrying Yraci on chromosome 1A identified 18 NBS-LRR genes based on wheat RefSeqv1.0 annotations. Our results indicate that as there is no major gene reported in the Yraci chromosome region, it is likely to be a novel stripe rust resistance locus and offers potential for deployment, using the identified markers, to confer all stage stripe rust resistance. Public Library of Science 2022-02-16 /pmc/articles/PMC8849526/ /pubmed/35171951 http://dx.doi.org/10.1371/journal.pone.0264027 Text en © 2022 Grover et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grover, Gomti
Sharma, Achla
Mackay, Ian
Srivastava, Puja
Kaur, Satinder
Kaur, Jaspal
Burridge, Amanda
Allen, Sacha Przewieslik
Bentley, Alison R.
Chhuneja, Parveen
Bains, N. S.
Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_full Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_fullStr Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_full_unstemmed Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_short Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation
title_sort identification of a novel stripe rust resistance gene from the european winter wheat cultivar ‘acienda’: a step towards rust proofing wheat cultivation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8849526/
https://www.ncbi.nlm.nih.gov/pubmed/35171951
http://dx.doi.org/10.1371/journal.pone.0264027
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