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Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice

This research aimed to determine the topical administration effect of the combination of Sargassum duplicatum and Garcinia mangostana extracts to ameliorate diabetic open wound healing. The study used 24 adult males of Mus musculus (BALB/c strain, 3–4 months, 30–40 g). They were divided into normal...

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Autores principales: Winarni, Dwi, Husna, Fitria Nikmatul, Syadzha, Muhammad Farraz, Susilo, Raden Joko Kuncoroningrat, Hayaza, Suhailah, Ansori, Arif Nur Muhammad, Alamsjah, Mochammad Amin, Amin, Muhamad Nur Ghoyatul, Wulandari, Pugar Arga Christina, Pudjiastuti, Pratiwi, Awang, Khalijah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850046/
https://www.ncbi.nlm.nih.gov/pubmed/35186344
http://dx.doi.org/10.1155/2022/9700794
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author Winarni, Dwi
Husna, Fitria Nikmatul
Syadzha, Muhammad Farraz
Susilo, Raden Joko Kuncoroningrat
Hayaza, Suhailah
Ansori, Arif Nur Muhammad
Alamsjah, Mochammad Amin
Amin, Muhamad Nur Ghoyatul
Wulandari, Pugar Arga Christina
Pudjiastuti, Pratiwi
Awang, Khalijah
author_facet Winarni, Dwi
Husna, Fitria Nikmatul
Syadzha, Muhammad Farraz
Susilo, Raden Joko Kuncoroningrat
Hayaza, Suhailah
Ansori, Arif Nur Muhammad
Alamsjah, Mochammad Amin
Amin, Muhamad Nur Ghoyatul
Wulandari, Pugar Arga Christina
Pudjiastuti, Pratiwi
Awang, Khalijah
author_sort Winarni, Dwi
collection PubMed
description This research aimed to determine the topical administration effect of the combination of Sargassum duplicatum and Garcinia mangostana extracts to ameliorate diabetic open wound healing. The study used 24 adult males of Mus musculus (BALB/c strain, 3–4 months, 30–40 g). They were divided into normal control groups (KN) and diabetic groups. The diabetic group was streptozotocin-induced and divided further into three treatment groups: the diabetic control group (KD), the S. duplicatum treatment group (PA), and the combination of S. duplicatum and G. mangostana treatment group (PAM). The dose of treatment was 50 mg/kg of body weight. Each group was divided into three treatment durations, which were 3 days, 7 days, and 14 days. The wound healing process was determined by wound width, the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density. Histological observation showed that the topical administration of combination extracts increased the re-epithelialization of the wounded area, fibroblasts, fibrocytes, and collagen synthesis. The topical administration of combination extracts also decreased the number of neutrophils and macrophages. This study concluded that the topical administration of the combination of S. duplicatum and G. mangostana extracts improved the open wound healing process in diabetic mice.
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spelling pubmed-88500462022-02-17 Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice Winarni, Dwi Husna, Fitria Nikmatul Syadzha, Muhammad Farraz Susilo, Raden Joko Kuncoroningrat Hayaza, Suhailah Ansori, Arif Nur Muhammad Alamsjah, Mochammad Amin Amin, Muhamad Nur Ghoyatul Wulandari, Pugar Arga Christina Pudjiastuti, Pratiwi Awang, Khalijah Scientifica (Cairo) Research Article This research aimed to determine the topical administration effect of the combination of Sargassum duplicatum and Garcinia mangostana extracts to ameliorate diabetic open wound healing. The study used 24 adult males of Mus musculus (BALB/c strain, 3–4 months, 30–40 g). They were divided into normal control groups (KN) and diabetic groups. The diabetic group was streptozotocin-induced and divided further into three treatment groups: the diabetic control group (KD), the S. duplicatum treatment group (PA), and the combination of S. duplicatum and G. mangostana treatment group (PAM). The dose of treatment was 50 mg/kg of body weight. Each group was divided into three treatment durations, which were 3 days, 7 days, and 14 days. The wound healing process was determined by wound width, the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density. Histological observation showed that the topical administration of combination extracts increased the re-epithelialization of the wounded area, fibroblasts, fibrocytes, and collagen synthesis. The topical administration of combination extracts also decreased the number of neutrophils and macrophages. This study concluded that the topical administration of the combination of S. duplicatum and G. mangostana extracts improved the open wound healing process in diabetic mice. Hindawi 2022-02-09 /pmc/articles/PMC8850046/ /pubmed/35186344 http://dx.doi.org/10.1155/2022/9700794 Text en Copyright © 2022 Dwi Winarni et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Winarni, Dwi
Husna, Fitria Nikmatul
Syadzha, Muhammad Farraz
Susilo, Raden Joko Kuncoroningrat
Hayaza, Suhailah
Ansori, Arif Nur Muhammad
Alamsjah, Mochammad Amin
Amin, Muhamad Nur Ghoyatul
Wulandari, Pugar Arga Christina
Pudjiastuti, Pratiwi
Awang, Khalijah
Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title_full Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title_fullStr Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title_full_unstemmed Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title_short Topical Administration Effect of Sargassum duplicatum and Garcinia mangostana Extracts Combination on Open Wound Healing Process in Diabetic Mice
title_sort topical administration effect of sargassum duplicatum and garcinia mangostana extracts combination on open wound healing process in diabetic mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850046/
https://www.ncbi.nlm.nih.gov/pubmed/35186344
http://dx.doi.org/10.1155/2022/9700794
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