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Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice

Circulating white blood cells (WBC) contribute toward maintenance of cerebral metabolism and brain function. Recently, we showed that during aging, transcription of metabolism related genes, including energy source transports, in the brain significantly decreased at the hippocampus resulting in impa...

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Autores principales: Takeuchi, Yukiko, Saino, Orie, Okinaka, Yuka, Ogawa, Yuko, Akamatsu, Rie, Kikuchi-Taura, Akie, Kataoka, Yosky, Maeda, Mitsuyo, Gul, Sheraz, Claussen, Carsten, Boltze, Johannes, Taguchi, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850360/
https://www.ncbi.nlm.nih.gov/pubmed/35185523
http://dx.doi.org/10.3389/fnagi.2022.759159
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author Takeuchi, Yukiko
Saino, Orie
Okinaka, Yuka
Ogawa, Yuko
Akamatsu, Rie
Kikuchi-Taura, Akie
Kataoka, Yosky
Maeda, Mitsuyo
Gul, Sheraz
Claussen, Carsten
Boltze, Johannes
Taguchi, Akihiko
author_facet Takeuchi, Yukiko
Saino, Orie
Okinaka, Yuka
Ogawa, Yuko
Akamatsu, Rie
Kikuchi-Taura, Akie
Kataoka, Yosky
Maeda, Mitsuyo
Gul, Sheraz
Claussen, Carsten
Boltze, Johannes
Taguchi, Akihiko
author_sort Takeuchi, Yukiko
collection PubMed
description Circulating white blood cells (WBC) contribute toward maintenance of cerebral metabolism and brain function. Recently, we showed that during aging, transcription of metabolism related genes, including energy source transports, in the brain significantly decreased at the hippocampus resulting in impaired neurological functions. In this article, we investigated the changes in RNA transcription of metabolism related genes (glucose transporter 1 [Glut1], Glut3, monocarboxylate transporter 4 [MCT4], hypoxia inducible factor 1-α [Hif1-α], prolyl hydroxylase 3 [PHD3] and pyruvate dehydrogenase kinase 1 [PDK1]) in circulating WBC and correlated these with brain function in mice. Contrary to our expectations, most of these metabolism related genes in circulating WBC significantly increased in aged mice, and correlation between their increased RNA transcription and impaired neurological functions was observed. Bone marrow mononuclear transplantation into aged mice decreased metabolism related genes in WBC with accelerated neurogenesis in the hippocampus. In vitro analysis revealed that cell-cell interaction between WBC and endothelial cells via gap junction is impaired with aging, and blockade of the interaction increased their transcription in WBC. Our findings indicate that gross analysis of RNA transcription of metabolism related genes in circulating WBC has the potential to provide significant information relating to impaired cell-cell interaction between WBC and endothelial cells of aged mice. Additionally, this can serve as a tool to evaluate the change of the cell-cell interaction caused by various treatments or diseases.
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spelling pubmed-88503602022-02-18 Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice Takeuchi, Yukiko Saino, Orie Okinaka, Yuka Ogawa, Yuko Akamatsu, Rie Kikuchi-Taura, Akie Kataoka, Yosky Maeda, Mitsuyo Gul, Sheraz Claussen, Carsten Boltze, Johannes Taguchi, Akihiko Front Aging Neurosci Neuroscience Circulating white blood cells (WBC) contribute toward maintenance of cerebral metabolism and brain function. Recently, we showed that during aging, transcription of metabolism related genes, including energy source transports, in the brain significantly decreased at the hippocampus resulting in impaired neurological functions. In this article, we investigated the changes in RNA transcription of metabolism related genes (glucose transporter 1 [Glut1], Glut3, monocarboxylate transporter 4 [MCT4], hypoxia inducible factor 1-α [Hif1-α], prolyl hydroxylase 3 [PHD3] and pyruvate dehydrogenase kinase 1 [PDK1]) in circulating WBC and correlated these with brain function in mice. Contrary to our expectations, most of these metabolism related genes in circulating WBC significantly increased in aged mice, and correlation between their increased RNA transcription and impaired neurological functions was observed. Bone marrow mononuclear transplantation into aged mice decreased metabolism related genes in WBC with accelerated neurogenesis in the hippocampus. In vitro analysis revealed that cell-cell interaction between WBC and endothelial cells via gap junction is impaired with aging, and blockade of the interaction increased their transcription in WBC. Our findings indicate that gross analysis of RNA transcription of metabolism related genes in circulating WBC has the potential to provide significant information relating to impaired cell-cell interaction between WBC and endothelial cells of aged mice. Additionally, this can serve as a tool to evaluate the change of the cell-cell interaction caused by various treatments or diseases. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850360/ /pubmed/35185523 http://dx.doi.org/10.3389/fnagi.2022.759159 Text en Copyright © 2022 Takeuchi, Saino, Okinaka, Ogawa, Akamatsu, Kikuchi-Taura, Kataoka, Maeda, Gul, Claussen, Boltze and Taguchi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Takeuchi, Yukiko
Saino, Orie
Okinaka, Yuka
Ogawa, Yuko
Akamatsu, Rie
Kikuchi-Taura, Akie
Kataoka, Yosky
Maeda, Mitsuyo
Gul, Sheraz
Claussen, Carsten
Boltze, Johannes
Taguchi, Akihiko
Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title_full Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title_fullStr Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title_full_unstemmed Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title_short Increased RNA Transcription of Energy Source Transporters in Circulating White Blood Cells of Aged Mice
title_sort increased rna transcription of energy source transporters in circulating white blood cells of aged mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850360/
https://www.ncbi.nlm.nih.gov/pubmed/35185523
http://dx.doi.org/10.3389/fnagi.2022.759159
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