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Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans

Many components in ovarian follicles (follicular fluid, cumulus cells, granular cells, etc.) dynamically change during folliculogenesis and play a positive or negative role in oocyte maturation. Infertile women who underwent intracytoplasmic sperm injection (ICSI) treatment in the reproductive medic...

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Autores principales: Wang, Chong, Fei, Xiaoyang, Zhang, Hongyan, Zhou, Wenjing, Cheng, Zhaojun, Feng, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850365/
https://www.ncbi.nlm.nih.gov/pubmed/35185790
http://dx.doi.org/10.3389/fendo.2021.830691
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author Wang, Chong
Fei, Xiaoyang
Zhang, Hongyan
Zhou, Wenjing
Cheng, Zhaojun
Feng, Ying
author_facet Wang, Chong
Fei, Xiaoyang
Zhang, Hongyan
Zhou, Wenjing
Cheng, Zhaojun
Feng, Ying
author_sort Wang, Chong
collection PubMed
description Many components in ovarian follicles (follicular fluid, cumulus cells, granular cells, etc.) dynamically change during folliculogenesis and play a positive or negative role in oocyte maturation. Infertile women who underwent intracytoplasmic sperm injection (ICSI) treatment in the reproductive medicine centre of Hangzhou Women’s Hospital between October 2018 and October 2021 were included. The ovarian follicular fluid and cumulus cells of diminished ovarian response (DOR) patients and control subjects with medical records of clinical data were collected. In total, 31 differentially expressed proteins, including 10 upregulated proteins (>1.50-fold, P<0.05) and 21 downregulated proteins (<0.67-fold, P<0.05), were identified in mature vs. immature oocytes by iTRAQ labelling coupled with 2D LC-MS/MS. GO analysis revealed that ‘cell population proliferation’ was the most diverse enrichment trend between up/downregulated proteins, while phagosome process and the PI3K-Akt signaling pathway were the two most significant pathways revealed by KEGG enrichment classification. Human prostatic acid phosphatase (PAP, ACPP) and CD5 antigen-like (CD5L) were two proteins verified by ELISA to be differentially expressed between MII and Gv oocytes (P<0.0001 and P<0.0001, respectively). Further measurement found significantly lower level of ACPP in follicular fluids and cumulus cells of DOR patients (P=0.028 and P=0.004, respectively), as an indicator of oocyte quality. Otherwise, CD5L level is upregulated in follicular fluid of DOR patients (P<0.0001). Our study provided experimental data to establish the objective indicator of oocyte maturation in the microenvironment of ovarian follicles, and also provided new insight into the measurement of oocyte quality.
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spelling pubmed-88503652022-02-18 Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans Wang, Chong Fei, Xiaoyang Zhang, Hongyan Zhou, Wenjing Cheng, Zhaojun Feng, Ying Front Endocrinol (Lausanne) Endocrinology Many components in ovarian follicles (follicular fluid, cumulus cells, granular cells, etc.) dynamically change during folliculogenesis and play a positive or negative role in oocyte maturation. Infertile women who underwent intracytoplasmic sperm injection (ICSI) treatment in the reproductive medicine centre of Hangzhou Women’s Hospital between October 2018 and October 2021 were included. The ovarian follicular fluid and cumulus cells of diminished ovarian response (DOR) patients and control subjects with medical records of clinical data were collected. In total, 31 differentially expressed proteins, including 10 upregulated proteins (>1.50-fold, P<0.05) and 21 downregulated proteins (<0.67-fold, P<0.05), were identified in mature vs. immature oocytes by iTRAQ labelling coupled with 2D LC-MS/MS. GO analysis revealed that ‘cell population proliferation’ was the most diverse enrichment trend between up/downregulated proteins, while phagosome process and the PI3K-Akt signaling pathway were the two most significant pathways revealed by KEGG enrichment classification. Human prostatic acid phosphatase (PAP, ACPP) and CD5 antigen-like (CD5L) were two proteins verified by ELISA to be differentially expressed between MII and Gv oocytes (P<0.0001 and P<0.0001, respectively). Further measurement found significantly lower level of ACPP in follicular fluids and cumulus cells of DOR patients (P=0.028 and P=0.004, respectively), as an indicator of oocyte quality. Otherwise, CD5L level is upregulated in follicular fluid of DOR patients (P<0.0001). Our study provided experimental data to establish the objective indicator of oocyte maturation in the microenvironment of ovarian follicles, and also provided new insight into the measurement of oocyte quality. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850365/ /pubmed/35185790 http://dx.doi.org/10.3389/fendo.2021.830691 Text en Copyright © 2022 Wang, Fei, Zhang, Zhou, Cheng and Feng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Wang, Chong
Fei, Xiaoyang
Zhang, Hongyan
Zhou, Wenjing
Cheng, Zhaojun
Feng, Ying
Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title_full Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title_fullStr Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title_full_unstemmed Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title_short Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans
title_sort proteomic analysis of the alterations in follicular fluid proteins during oocyte maturation in humans
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850365/
https://www.ncbi.nlm.nih.gov/pubmed/35185790
http://dx.doi.org/10.3389/fendo.2021.830691
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