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CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction

Development of hyperproducing strains is important for biomanufacturing of biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and discover functional components. Herein, we optimize and use the CRISPR-assisted editing and CRISPRi screening methods to convert a w...

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Detalles Bibliográficos
Autores principales:	Liu, Jiao, Liu, Moshi, Shi, Tuo, Sun, Guannan, Gao, Ning, Zhao, Xiaojia, Guo, Xuan, Ni, Xiaomeng, Yuan, Qianqian, Feng, Jinhui, Liu, Zhemin, Guo, Yanmei, Chen, Jiuzhou, Wang, Yu, Zheng, Ping, Sun, Jibin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850433/ https://www.ncbi.nlm.nih.gov/pubmed/35173152 http://dx.doi.org/10.1038/s41467-022-28501-7

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