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Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats

Honeybee products consist of many substances, which have long been known for their medicinal and health-promoting properties. This study set out to appraise the protective potential of Egyptian propolis (EP) and bee venom (BV) separately or combined against total body irradiation (TBI) induced oxida...

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Autores principales: El Adham, Eithar K., Hassan, Amal I., A. Dawoud, M. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850618/
https://www.ncbi.nlm.nih.gov/pubmed/35173181
http://dx.doi.org/10.1038/s41598-022-05979-1
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author El Adham, Eithar K.
Hassan, Amal I.
A. Dawoud, M. M.
author_facet El Adham, Eithar K.
Hassan, Amal I.
A. Dawoud, M. M.
author_sort El Adham, Eithar K.
collection PubMed
description Honeybee products consist of many substances, which have long been known for their medicinal and health-promoting properties. This study set out to appraise the protective potential of Egyptian propolis (EP) and bee venom (BV) separately or combined against total body irradiation (TBI) induced oxidative injury in rats. Besides, we assessed the bioactive components in EP and BV using HPLC and UPLC/ ESI–MS analysis in the positive ion mode. The animals were subjected to a source of gamma ionizing radiation at a dose of 6 Gy. Propolis and BV were administered independently and in combination before 14 days of γ-irradiation. Liver and kidney functions were estimated besides, DNA damage index (8- OHdG) by ELISA. Antioxidants, including glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were detected. Gene expression technique investigated for BAX, BCL2, and in plasma also miR125b expression in serum of rats. Besides, the histopathological for the brain, liver, kidney, and heart were investigated. In addition, lipid peroxidation was investigated in plasma and in the previous organs. The present results provide opportunities to advance the use of bee products as promising medicinal sources.
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spelling pubmed-88506182022-02-18 Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats El Adham, Eithar K. Hassan, Amal I. A. Dawoud, M. M. Sci Rep Article Honeybee products consist of many substances, which have long been known for their medicinal and health-promoting properties. This study set out to appraise the protective potential of Egyptian propolis (EP) and bee venom (BV) separately or combined against total body irradiation (TBI) induced oxidative injury in rats. Besides, we assessed the bioactive components in EP and BV using HPLC and UPLC/ ESI–MS analysis in the positive ion mode. The animals were subjected to a source of gamma ionizing radiation at a dose of 6 Gy. Propolis and BV were administered independently and in combination before 14 days of γ-irradiation. Liver and kidney functions were estimated besides, DNA damage index (8- OHdG) by ELISA. Antioxidants, including glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were detected. Gene expression technique investigated for BAX, BCL2, and in plasma also miR125b expression in serum of rats. Besides, the histopathological for the brain, liver, kidney, and heart were investigated. In addition, lipid peroxidation was investigated in plasma and in the previous organs. The present results provide opportunities to advance the use of bee products as promising medicinal sources. Nature Publishing Group UK 2022-02-16 /pmc/articles/PMC8850618/ /pubmed/35173181 http://dx.doi.org/10.1038/s41598-022-05979-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
El Adham, Eithar K.
Hassan, Amal I.
A. Dawoud, M. M.
Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title_full Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title_fullStr Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title_full_unstemmed Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title_short Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
title_sort evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850618/
https://www.ncbi.nlm.nih.gov/pubmed/35173181
http://dx.doi.org/10.1038/s41598-022-05979-1
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