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Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle

Nitroethane is a potent methane-inhibitor for ruminants but little is known regarding simultaneous effects of repeated administration on pre- and post-gastric methane-producing activity and potential absorption and systemic accumulation of nitroethane in ruminants. Intraruminal administration of 120...

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Autores principales: Božic, Aleksandar K., Gutiérrez-Bañuelos, Hector, Corral-Luna, Agustin, Carstens, Gordon, Arévalos-Sánchez, Martha María, Félix-Portillo, Monserrath, Muro-Reyes, Alberto, Arzola-Álvarez, Claudio, Anderson, Robin C., Harvey, Roger B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850640/
https://www.ncbi.nlm.nih.gov/pubmed/35187146
http://dx.doi.org/10.3389/fvets.2022.817270
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author Božic, Aleksandar K.
Gutiérrez-Bañuelos, Hector
Corral-Luna, Agustin
Carstens, Gordon
Arévalos-Sánchez, Martha María
Félix-Portillo, Monserrath
Muro-Reyes, Alberto
Arzola-Álvarez, Claudio
Anderson, Robin C.
Harvey, Roger B.
author_facet Božic, Aleksandar K.
Gutiérrez-Bañuelos, Hector
Corral-Luna, Agustin
Carstens, Gordon
Arévalos-Sánchez, Martha María
Félix-Portillo, Monserrath
Muro-Reyes, Alberto
Arzola-Álvarez, Claudio
Anderson, Robin C.
Harvey, Roger B.
author_sort Božic, Aleksandar K.
collection PubMed
description Nitroethane is a potent methane-inhibitor for ruminants but little is known regarding simultaneous effects of repeated administration on pre- and post-gastric methane-producing activity and potential absorption and systemic accumulation of nitroethane in ruminants. Intraruminal administration of 120 mg nitroethane/kg body weight per day to Holstein cows (n = 2) over a 4-day period transiently reduced (P < 0.05) methane-producing activity of rumen fluid as much as 3.6-fold while concomitantly increasing (P < 0.05) methane-producing activity of feces by as much as 8.8-fold when compared to pre-treatment measurements. These observations suggest a bacteriostatic effect of nitroethane on ruminal methanogen populations resulting in increased passage of viable methanogens to the lower bovine gut. Ruminal VFA concentrations were also transiently affected by nitroethane administration (P < 0.05) reflecting adaptive changes in the rumen microbial populations. Mean (± SD) nitroethane concentrations in plasma of feedlot steers (n = 6/treatment) administered 80 or 160 mg nitroethane/kg body weight per day over a 7-day period were 0.12 ± 0.1 and 0.41 ± 0.1 μmol/mL 8 h after the initial administration indicating rapid absorption of nitroethane, with concentrations peaking 1 day after initiation of the 80 or 160 mg nitroethane/kg body weight per day treatments (0.38 ± 0.1 and 1.14 ± 0.1 μmol/mL, respectively). Plasma nitroethane concentrations declined thereafter to 0.25 ± 0.1 and 0.78 ± 0.3 and to 0.18 ± 0.1 and 0.44 ± 0.3 μmol/mL on days 2 and 7 for the 80 or 160 mg nitroethane/kg body weight per day treatment groups, respectively, indicating decreased absorption due to increased ruminal nitroethane degradation or to more rapid excretion of the compound.
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spelling pubmed-88506402022-02-18 Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle Božic, Aleksandar K. Gutiérrez-Bañuelos, Hector Corral-Luna, Agustin Carstens, Gordon Arévalos-Sánchez, Martha María Félix-Portillo, Monserrath Muro-Reyes, Alberto Arzola-Álvarez, Claudio Anderson, Robin C. Harvey, Roger B. Front Vet Sci Veterinary Science Nitroethane is a potent methane-inhibitor for ruminants but little is known regarding simultaneous effects of repeated administration on pre- and post-gastric methane-producing activity and potential absorption and systemic accumulation of nitroethane in ruminants. Intraruminal administration of 120 mg nitroethane/kg body weight per day to Holstein cows (n = 2) over a 4-day period transiently reduced (P < 0.05) methane-producing activity of rumen fluid as much as 3.6-fold while concomitantly increasing (P < 0.05) methane-producing activity of feces by as much as 8.8-fold when compared to pre-treatment measurements. These observations suggest a bacteriostatic effect of nitroethane on ruminal methanogen populations resulting in increased passage of viable methanogens to the lower bovine gut. Ruminal VFA concentrations were also transiently affected by nitroethane administration (P < 0.05) reflecting adaptive changes in the rumen microbial populations. Mean (± SD) nitroethane concentrations in plasma of feedlot steers (n = 6/treatment) administered 80 or 160 mg nitroethane/kg body weight per day over a 7-day period were 0.12 ± 0.1 and 0.41 ± 0.1 μmol/mL 8 h after the initial administration indicating rapid absorption of nitroethane, with concentrations peaking 1 day after initiation of the 80 or 160 mg nitroethane/kg body weight per day treatments (0.38 ± 0.1 and 1.14 ± 0.1 μmol/mL, respectively). Plasma nitroethane concentrations declined thereafter to 0.25 ± 0.1 and 0.78 ± 0.3 and to 0.18 ± 0.1 and 0.44 ± 0.3 μmol/mL on days 2 and 7 for the 80 or 160 mg nitroethane/kg body weight per day treatment groups, respectively, indicating decreased absorption due to increased ruminal nitroethane degradation or to more rapid excretion of the compound. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850640/ /pubmed/35187146 http://dx.doi.org/10.3389/fvets.2022.817270 Text en Copyright © 2022 Božic, Gutiérrez-Bañuelos, Corral-Luna, Carstens, Arévalos-Sánchez, Félix-Portillo, Muro-Reyes, Arzola-Álvarez, Anderson and Harvey. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Božic, Aleksandar K.
Gutiérrez-Bañuelos, Hector
Corral-Luna, Agustin
Carstens, Gordon
Arévalos-Sánchez, Martha María
Félix-Portillo, Monserrath
Muro-Reyes, Alberto
Arzola-Álvarez, Claudio
Anderson, Robin C.
Harvey, Roger B.
Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title_full Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title_fullStr Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title_full_unstemmed Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title_short Dynamics of Gastrointestinal Activity and Ruminal Absorption of the Methane-Inhibitor, Nitroethane, in Cattle
title_sort dynamics of gastrointestinal activity and ruminal absorption of the methane-inhibitor, nitroethane, in cattle
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850640/
https://www.ncbi.nlm.nih.gov/pubmed/35187146
http://dx.doi.org/10.3389/fvets.2022.817270
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