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GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean

Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered m...

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Autores principales: Mu, Ruolan, Lyu, Xiangguang, Ji, Ronghuan, Liu, Jun, Zhao, Tao, Li, Hongyu, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850649/
https://www.ncbi.nlm.nih.gov/pubmed/35185980
http://dx.doi.org/10.3389/fpls.2022.803122
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author Mu, Ruolan
Lyu, Xiangguang
Ji, Ronghuan
Liu, Jun
Zhao, Tao
Li, Hongyu
Liu, Bin
author_facet Mu, Ruolan
Lyu, Xiangguang
Ji, Ronghuan
Liu, Jun
Zhao, Tao
Li, Hongyu
Liu, Bin
author_sort Mu, Ruolan
collection PubMed
description Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future.
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spelling pubmed-88506492022-02-18 GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean Mu, Ruolan Lyu, Xiangguang Ji, Ronghuan Liu, Jun Zhao, Tao Li, Hongyu Liu, Bin Front Plant Sci Plant Science Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850649/ /pubmed/35185980 http://dx.doi.org/10.3389/fpls.2022.803122 Text en Copyright © 2022 Mu, Lyu, Ji, Liu, Zhao, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mu, Ruolan
Lyu, Xiangguang
Ji, Ronghuan
Liu, Jun
Zhao, Tao
Li, Hongyu
Liu, Bin
GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title_full GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title_fullStr GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title_full_unstemmed GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title_short GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
title_sort gmbics modulate low blue light-induced stem elongation in soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850649/
https://www.ncbi.nlm.nih.gov/pubmed/35185980
http://dx.doi.org/10.3389/fpls.2022.803122
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