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GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean
Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered m...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850649/ https://www.ncbi.nlm.nih.gov/pubmed/35185980 http://dx.doi.org/10.3389/fpls.2022.803122 |
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author | Mu, Ruolan Lyu, Xiangguang Ji, Ronghuan Liu, Jun Zhao, Tao Li, Hongyu Liu, Bin |
author_facet | Mu, Ruolan Lyu, Xiangguang Ji, Ronghuan Liu, Jun Zhao, Tao Li, Hongyu Liu, Bin |
author_sort | Mu, Ruolan |
collection | PubMed |
description | Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future. |
format | Online Article Text |
id | pubmed-8850649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88506492022-02-18 GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean Mu, Ruolan Lyu, Xiangguang Ji, Ronghuan Liu, Jun Zhao, Tao Li, Hongyu Liu, Bin Front Plant Sci Plant Science Blue-light inhibitors of cryptochromes (BICs) promote hypocotyl elongation by suppressing the activity of cryptochromes in Arabidopsis. Nevertheless, the roles of BICs in other plant species are still unclear. Here we investigate their functions by genetic overexpression and CRISPR/Cas9 engineered mutations targeting the six GmBIC genes in soybean. We showed that the GmBICs overexpression (GmBICs-OX) lines strongly promoted stem elongation, while the single, double, and quadruple mutations in the GmBIC genes resulted in incremental dwarfing phenotypes. Furthermore, overexpression of GmBIC2a abolished the low blue light (LBL)-induced stem elongation, demonstrating the involvement of GmBICs in regulating cryptochrome-mediated LBL-induced shade avoidance syndrome (SAS). The Gmbic1a1b2a2b quadruple mutant displayed reduced stem elongation under LBL conditions, which was reminiscent of the GmCRY1b-OX lines. Taken together, this study provided essential genetic resources for elucidating GmBICs functional mechanisms and breeding of shade-tolerant soybean cultivars in future. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850649/ /pubmed/35185980 http://dx.doi.org/10.3389/fpls.2022.803122 Text en Copyright © 2022 Mu, Lyu, Ji, Liu, Zhao, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mu, Ruolan Lyu, Xiangguang Ji, Ronghuan Liu, Jun Zhao, Tao Li, Hongyu Liu, Bin GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title | GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title_full | GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title_fullStr | GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title_full_unstemmed | GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title_short | GmBICs Modulate Low Blue Light-Induced Stem Elongation in Soybean |
title_sort | gmbics modulate low blue light-induced stem elongation in soybean |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850649/ https://www.ncbi.nlm.nih.gov/pubmed/35185980 http://dx.doi.org/10.3389/fpls.2022.803122 |
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