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LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian

OBJECTIVE: The study aimed to delineate the gene expression profile of LGR5, HES1 and ATOH1 in young Egyptian rectal cancer (RC) patients and investigate the correlation between expression profiles and clinical outcome. METHODS: The study was conducted on 30 young Egyptian RC patients. Expression st...

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Autores principales: Morsy, Heba, Gaballah, Ahmed, Samir, Mohamed, Nakundi, Vandrome, Shamseya, Mohamed, Mahrous, Hanan, Ghazal, Abeer, Hashish, Mervat, Arafat, Waleed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850894/
https://www.ncbi.nlm.nih.gov/pubmed/34582650
http://dx.doi.org/10.31557/APJCP.2021.22.9.2819
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author Morsy, Heba
Gaballah, Ahmed
Samir, Mohamed
Nakundi, Vandrome
Shamseya, Mohamed
Mahrous, Hanan
Ghazal, Abeer
Hashish, Mervat
Arafat, Waleed
author_facet Morsy, Heba
Gaballah, Ahmed
Samir, Mohamed
Nakundi, Vandrome
Shamseya, Mohamed
Mahrous, Hanan
Ghazal, Abeer
Hashish, Mervat
Arafat, Waleed
author_sort Morsy, Heba
collection PubMed
description OBJECTIVE: The study aimed to delineate the gene expression profile of LGR5, HES1 and ATOH1 in young Egyptian rectal cancer (RC) patients and investigate the correlation between expression profiles and clinical outcome. METHODS: The study was conducted on 30 young Egyptian RC patients. Expression study of LGR5, HES1 and ATOH1 were performed by quantitative PCR (QPCR) based on comparative Cq method after normalization to adjacent non tumor tissues and ACTB as a reference gene. Patients were followed up for assessment of response to neoadjuvant chemoradiotherapy (CRT) based on revised RECIST1.1. RESULT: The study detected overexpression of LGR5 and HES1 and down-regulation of ATOH1 in human RC tissues compared to non- tumor tissues. High expression of LGR5 was correlated with more depth of tumor invasion, lymph node (LN) metastasis, advanced cTNM stage and mesorectal fascia (MRF) involvement. More prominently, high LGR5 expression level was associated with poor response to CRT. LGR5 was suggested as unfavorable prognostic biomarker for RC. Conversely, HES1 and ATOH1 expression did not show significant association with most of the studied clinical criteria nor response to CRT. Still, HES1 and ATOH1 were significantly and inversely associated with presence of mucinous component. CONCLUSION: High LGR5 expression is indicative of poor prognosis among young Egyptian RC patients and is proposed as a predictive marker of resistance to neoadjuvant CRT. However, HES1 and ATOH1 expressions were not prognostic nor predictive of response to CRT. Overall, LGR5, HES1 and ATOH1 gene expression patterns among young onset RC patients, are in line with patterns encountered in older age groups.
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spelling pubmed-88508942022-02-24 LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian Morsy, Heba Gaballah, Ahmed Samir, Mohamed Nakundi, Vandrome Shamseya, Mohamed Mahrous, Hanan Ghazal, Abeer Hashish, Mervat Arafat, Waleed Asian Pac J Cancer Prev Research Article OBJECTIVE: The study aimed to delineate the gene expression profile of LGR5, HES1 and ATOH1 in young Egyptian rectal cancer (RC) patients and investigate the correlation between expression profiles and clinical outcome. METHODS: The study was conducted on 30 young Egyptian RC patients. Expression study of LGR5, HES1 and ATOH1 were performed by quantitative PCR (QPCR) based on comparative Cq method after normalization to adjacent non tumor tissues and ACTB as a reference gene. Patients were followed up for assessment of response to neoadjuvant chemoradiotherapy (CRT) based on revised RECIST1.1. RESULT: The study detected overexpression of LGR5 and HES1 and down-regulation of ATOH1 in human RC tissues compared to non- tumor tissues. High expression of LGR5 was correlated with more depth of tumor invasion, lymph node (LN) metastasis, advanced cTNM stage and mesorectal fascia (MRF) involvement. More prominently, high LGR5 expression level was associated with poor response to CRT. LGR5 was suggested as unfavorable prognostic biomarker for RC. Conversely, HES1 and ATOH1 expression did not show significant association with most of the studied clinical criteria nor response to CRT. Still, HES1 and ATOH1 were significantly and inversely associated with presence of mucinous component. CONCLUSION: High LGR5 expression is indicative of poor prognosis among young Egyptian RC patients and is proposed as a predictive marker of resistance to neoadjuvant CRT. However, HES1 and ATOH1 expressions were not prognostic nor predictive of response to CRT. Overall, LGR5, HES1 and ATOH1 gene expression patterns among young onset RC patients, are in line with patterns encountered in older age groups. West Asia Organization for Cancer Prevention 2021-09 /pmc/articles/PMC8850894/ /pubmed/34582650 http://dx.doi.org/10.31557/APJCP.2021.22.9.2819 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Morsy, Heba
Gaballah, Ahmed
Samir, Mohamed
Nakundi, Vandrome
Shamseya, Mohamed
Mahrous, Hanan
Ghazal, Abeer
Hashish, Mervat
Arafat, Waleed
LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title_full LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title_fullStr LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title_full_unstemmed LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title_short LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian
title_sort lgr5, hes1 and atoh1 in young rectal cancer patients in egyptian
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850894/
https://www.ncbi.nlm.nih.gov/pubmed/34582650
http://dx.doi.org/10.31557/APJCP.2021.22.9.2819
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