Cargando…

Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling

The abnormal proliferation and migration of Vascular smooth muscle cells (VSMCs) are related to many cardiovascular diseases, including atherosclerosis, restenosis after balloon angioplasty, hypertension, etc. Myricanol is a diarylheptanoid that can be separated from the bark of Myrica rubra. It has...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Siyuan, Wang, Cheng, Huang, Kai, Liang, Minglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850918/
https://www.ncbi.nlm.nih.gov/pubmed/35185599
http://dx.doi.org/10.3389/fphys.2021.790345
_version_ 1784652709764268032
author Fan, Siyuan
Wang, Cheng
Huang, Kai
Liang, Minglu
author_facet Fan, Siyuan
Wang, Cheng
Huang, Kai
Liang, Minglu
author_sort Fan, Siyuan
collection PubMed
description The abnormal proliferation and migration of Vascular smooth muscle cells (VSMCs) are related to many cardiovascular diseases, including atherosclerosis, restenosis after balloon angioplasty, hypertension, etc. Myricanol is a diarylheptanoid that can be separated from the bark of Myrica rubra. It has been reported that myricanol can anti-inflammatory, anti-cancer, anti-neurodegenerative, promote autophagic clearance of tau and prevent muscle atrophy. But its potential role in the cardiovascular field remains unknown. In this study, we investigated the effect of myricanol on the proliferation and migration of VSMCs in vitro and on the intimal hyperplasia in vivo. In vitro experiments, we found myricanol can inhibit the proliferation and migration of VSMCs induced by PDGF-BB. In terms of mechanism, the preincubation of myricanol can suppress the PDGF-BB induced phosphorylation of PDGFRβ and its downstream such as PLCγ1, Src, and MAPKs. In addition, NF-kB p65 translocation was also suppressed by myricanol. In vivo experiments, we found myricanol can suppress the intimal hyperplasia after wire ligation of the carotid artery in mice. These results may provide a new strategy for the prevention and treatment of coronary atherosclerosis and post-stent stenosis in the future.
format Online
Article
Text
id pubmed-8850918
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88509182022-02-18 Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling Fan, Siyuan Wang, Cheng Huang, Kai Liang, Minglu Front Physiol Physiology The abnormal proliferation and migration of Vascular smooth muscle cells (VSMCs) are related to many cardiovascular diseases, including atherosclerosis, restenosis after balloon angioplasty, hypertension, etc. Myricanol is a diarylheptanoid that can be separated from the bark of Myrica rubra. It has been reported that myricanol can anti-inflammatory, anti-cancer, anti-neurodegenerative, promote autophagic clearance of tau and prevent muscle atrophy. But its potential role in the cardiovascular field remains unknown. In this study, we investigated the effect of myricanol on the proliferation and migration of VSMCs in vitro and on the intimal hyperplasia in vivo. In vitro experiments, we found myricanol can inhibit the proliferation and migration of VSMCs induced by PDGF-BB. In terms of mechanism, the preincubation of myricanol can suppress the PDGF-BB induced phosphorylation of PDGFRβ and its downstream such as PLCγ1, Src, and MAPKs. In addition, NF-kB p65 translocation was also suppressed by myricanol. In vivo experiments, we found myricanol can suppress the intimal hyperplasia after wire ligation of the carotid artery in mice. These results may provide a new strategy for the prevention and treatment of coronary atherosclerosis and post-stent stenosis in the future. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850918/ /pubmed/35185599 http://dx.doi.org/10.3389/fphys.2021.790345 Text en Copyright © 2022 Fan, Wang, Huang and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Fan, Siyuan
Wang, Cheng
Huang, Kai
Liang, Minglu
Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title_full Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title_fullStr Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title_full_unstemmed Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title_short Myricanol Inhibits Platelet Derived Growth Factor-BB-Induced Vascular Smooth Muscle Cells Proliferation and Migration in vitro and Intimal Hyperplasia in vivo by Targeting the Platelet-Derived Growth Factor Receptor-β and NF-κB Signaling
title_sort myricanol inhibits platelet derived growth factor-bb-induced vascular smooth muscle cells proliferation and migration in vitro and intimal hyperplasia in vivo by targeting the platelet-derived growth factor receptor-β and nf-κb signaling
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850918/
https://www.ncbi.nlm.nih.gov/pubmed/35185599
http://dx.doi.org/10.3389/fphys.2021.790345
work_keys_str_mv AT fansiyuan myricanolinhibitsplateletderivedgrowthfactorbbinducedvascularsmoothmusclecellsproliferationandmigrationinvitroandintimalhyperplasiainvivobytargetingtheplateletderivedgrowthfactorreceptorbandnfkbsignaling
AT wangcheng myricanolinhibitsplateletderivedgrowthfactorbbinducedvascularsmoothmusclecellsproliferationandmigrationinvitroandintimalhyperplasiainvivobytargetingtheplateletderivedgrowthfactorreceptorbandnfkbsignaling
AT huangkai myricanolinhibitsplateletderivedgrowthfactorbbinducedvascularsmoothmusclecellsproliferationandmigrationinvitroandintimalhyperplasiainvivobytargetingtheplateletderivedgrowthfactorreceptorbandnfkbsignaling
AT liangminglu myricanolinhibitsplateletderivedgrowthfactorbbinducedvascularsmoothmusclecellsproliferationandmigrationinvitroandintimalhyperplasiainvivobytargetingtheplateletderivedgrowthfactorreceptorbandnfkbsignaling