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Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues

Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid,...

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Detalles Bibliográficos
Autores principales: Zhu, Xiaoping, Xu, Tianyi, Peng, Chen, Wu, Shihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850921/
https://www.ncbi.nlm.nih.gov/pubmed/35186891
http://dx.doi.org/10.3389/fchem.2021.782432
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author Zhu, Xiaoping
Xu, Tianyi
Peng, Chen
Wu, Shihua
author_facet Zhu, Xiaoping
Xu, Tianyi
Peng, Chen
Wu, Shihua
author_sort Zhu, Xiaoping
collection PubMed
description Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still the most widespread MSI scanning method. Here, we aim to provide a comprehensive review of MALDI-MSI with an emphasis on its advances of the instrumentation, methods, application, and future directions in single cell and biological tissues.
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spelling pubmed-88509212022-02-18 Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues Zhu, Xiaoping Xu, Tianyi Peng, Chen Wu, Shihua Front Chem Chemistry Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still the most widespread MSI scanning method. Here, we aim to provide a comprehensive review of MALDI-MSI with an emphasis on its advances of the instrumentation, methods, application, and future directions in single cell and biological tissues. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850921/ /pubmed/35186891 http://dx.doi.org/10.3389/fchem.2021.782432 Text en Copyright © 2022 Zhu, Xu, Peng and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Zhu, Xiaoping
Xu, Tianyi
Peng, Chen
Wu, Shihua
Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title_full Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title_fullStr Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title_full_unstemmed Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title_short Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues
title_sort advances in maldi mass spectrometry imaging single cell and tissues
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850921/
https://www.ncbi.nlm.nih.gov/pubmed/35186891
http://dx.doi.org/10.3389/fchem.2021.782432
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