Cargando…

B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats

The accumulation of DNA damage in burn wounds delays wound healing. DNA methylation by short interspersed nuclear element (SINE) small interfering (si)RNA prevents DNA damage and promotes cell proliferation. Therefore, SINE siRNA may be able to promote burn wound healing. Here, a SINE B1 siRNA was u...

Descripción completa

Detalles Bibliográficos
Autores principales: Meevassana, Jiraroch, Nacharoenkul, Panupong, Wititsuwannakul, Jade, Kitkumthorn, Nakarin, Hamill, Kevin J., Angspatt, Apichai, Mutirangura, Apiwat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850962/
https://www.ncbi.nlm.nih.gov/pubmed/35251607
http://dx.doi.org/10.3892/br.2022.1503
_version_ 1784652722638684160
author Meevassana, Jiraroch
Nacharoenkul, Panupong
Wititsuwannakul, Jade
Kitkumthorn, Nakarin
Hamill, Kevin J.
Angspatt, Apichai
Mutirangura, Apiwat
author_facet Meevassana, Jiraroch
Nacharoenkul, Panupong
Wititsuwannakul, Jade
Kitkumthorn, Nakarin
Hamill, Kevin J.
Angspatt, Apichai
Mutirangura, Apiwat
author_sort Meevassana, Jiraroch
collection PubMed
description The accumulation of DNA damage in burn wounds delays wound healing. DNA methylation by short interspersed nuclear element (SINE) small interfering (si)RNA prevents DNA damage and promotes cell proliferation. Therefore, SINE siRNA may be able to promote burn wound healing. Here, a SINE B1 siRNA was used to treat burn wounds in rats. Second-degree burn wounds were introduced on the backs of rats. The rats were then divided into three groups: a B1 siRNA-treated, saline-treated control, and saline + calcium phosphate-nanoparticle-treated control group (n=15/group). The wounds were imaged on days 0, 7, 14, 21 and 28 post-injury. The tissue sections were processed for methylation, histological and immunohistochemical examination, and scored based on the overall expression of histone H2AX phosphorylated on serine 139 (γH2AX) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Burn wound closure improved in the B1 siRNA-treated group compared with that in the control group, especially from days 14-28 post-injury (P<0.001). The overall pathological score and degree of B1 methylation in the B1 siRNA-treated group improved significantly at days 14-28 post-injury, with the maximum improvement observed on day 14 (P<0.01) compared with the NSS and Ca-P nanoparticle groups. Immunohistochemical staining revealed lower expression of γH2AX and 8-OHdG in the B1 siRNA-treated group than in the control groups at days 14-28 post-injury; the maximum improvement was observed on days 14 and 21. These data imply that administering SINE siRNA is a promising therapeutic option for managing second-degree burns.
format Online
Article
Text
id pubmed-8850962
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-88509622022-03-03 B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats Meevassana, Jiraroch Nacharoenkul, Panupong Wititsuwannakul, Jade Kitkumthorn, Nakarin Hamill, Kevin J. Angspatt, Apichai Mutirangura, Apiwat Biomed Rep Articles The accumulation of DNA damage in burn wounds delays wound healing. DNA methylation by short interspersed nuclear element (SINE) small interfering (si)RNA prevents DNA damage and promotes cell proliferation. Therefore, SINE siRNA may be able to promote burn wound healing. Here, a SINE B1 siRNA was used to treat burn wounds in rats. Second-degree burn wounds were introduced on the backs of rats. The rats were then divided into three groups: a B1 siRNA-treated, saline-treated control, and saline + calcium phosphate-nanoparticle-treated control group (n=15/group). The wounds were imaged on days 0, 7, 14, 21 and 28 post-injury. The tissue sections were processed for methylation, histological and immunohistochemical examination, and scored based on the overall expression of histone H2AX phosphorylated on serine 139 (γH2AX) and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Burn wound closure improved in the B1 siRNA-treated group compared with that in the control group, especially from days 14-28 post-injury (P<0.001). The overall pathological score and degree of B1 methylation in the B1 siRNA-treated group improved significantly at days 14-28 post-injury, with the maximum improvement observed on day 14 (P<0.01) compared with the NSS and Ca-P nanoparticle groups. Immunohistochemical staining revealed lower expression of γH2AX and 8-OHdG in the B1 siRNA-treated group than in the control groups at days 14-28 post-injury; the maximum improvement was observed on days 14 and 21. These data imply that administering SINE siRNA is a promising therapeutic option for managing second-degree burns. D.A. Spandidos 2022-03 2022-02-03 /pmc/articles/PMC8850962/ /pubmed/35251607 http://dx.doi.org/10.3892/br.2022.1503 Text en Copyright: © Meevassana et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Meevassana, Jiraroch
Nacharoenkul, Panupong
Wititsuwannakul, Jade
Kitkumthorn, Nakarin
Hamill, Kevin J.
Angspatt, Apichai
Mutirangura, Apiwat
B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title_full B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title_fullStr B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title_full_unstemmed B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title_short B1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
title_sort b1 repetitive sequence methylation enhances wound healing of second-degree burns in rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850962/
https://www.ncbi.nlm.nih.gov/pubmed/35251607
http://dx.doi.org/10.3892/br.2022.1503
work_keys_str_mv AT meevassanajiraroch b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT nacharoenkulpanupong b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT wititsuwannakuljade b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT kitkumthornnakarin b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT hamillkevinj b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT angspattapichai b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats
AT mutiranguraapiwat b1repetitivesequencemethylationenhanceswoundhealingofseconddegreeburnsinrats