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Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes
Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added to existing transgenic lines, site-specific integration can stack new transgenes into a previously cr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850970/ https://www.ncbi.nlm.nih.gov/pubmed/35185992 http://dx.doi.org/10.3389/fpls.2022.828960 |
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author | Yin, Qian Li, Ruyu Ow, David W. |
author_facet | Yin, Qian Li, Ruyu Ow, David W. |
author_sort | Yin, Qian |
collection | PubMed |
description | Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added to existing transgenic lines, site-specific integration can stack new transgenes into a previously created transgenic locus. In planta site-specific integration minimizes the number of segregating loci to assemble into a breeding line, but cannot break genetic linkage between the transgenic locus and nearby undesirable traits. In this study, we describe an additional feature of an in planta gene-stacking scheme, in which the Cre (control of recombination) recombinase not only deletes transgenic DNA no longer needed after transformation but also mediates recombination between homologous or non-homologous chromosomes. Although the target site must first be introgressed through conventional breeding, subsequent transgenes inserted into the same locus would be able to use Cre-mediated translocation to expedite a linkage drag-free introgression to field cultivars. |
format | Online Article Text |
id | pubmed-8850970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88509702022-02-18 Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes Yin, Qian Li, Ruyu Ow, David W. Front Plant Sci Plant Science Transgene integration typically takes place in an easy-to-transform laboratory variety before the transformation event is introgressed through backcrosses to elite cultivars. As new traits are added to existing transgenic lines, site-specific integration can stack new transgenes into a previously created transgenic locus. In planta site-specific integration minimizes the number of segregating loci to assemble into a breeding line, but cannot break genetic linkage between the transgenic locus and nearby undesirable traits. In this study, we describe an additional feature of an in planta gene-stacking scheme, in which the Cre (control of recombination) recombinase not only deletes transgenic DNA no longer needed after transformation but also mediates recombination between homologous or non-homologous chromosomes. Although the target site must first be introgressed through conventional breeding, subsequent transgenes inserted into the same locus would be able to use Cre-mediated translocation to expedite a linkage drag-free introgression to field cultivars. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850970/ /pubmed/35185992 http://dx.doi.org/10.3389/fpls.2022.828960 Text en Copyright © 2022 Yin, Li and Ow. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Yin, Qian Li, Ruyu Ow, David W. Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title | Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title_full | Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title_fullStr | Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title_full_unstemmed | Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title_short | Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes |
title_sort | site-specific sequence exchange between homologous and non-homologous chromosomes |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850970/ https://www.ncbi.nlm.nih.gov/pubmed/35185992 http://dx.doi.org/10.3389/fpls.2022.828960 |
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