Cargando…

Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops

A large subset of plant stress-signaling pathways, including those related with chemical defense production, exhibit diurnal or circadian oscillations. However the extent to which diurnal or circadian time influences the stress mediated accumulation of plant specialized metabolites remains largely u...

Descripción completa

Detalles Bibliográficos
Autores principales: Doghri, Maroua, Rodríguez, Víctor Manuel, Kliebenstein, Daniel J., Francisco, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850993/
https://www.ncbi.nlm.nih.gov/pubmed/35185956
http://dx.doi.org/10.3389/fpls.2021.807710
_version_ 1784652733021683712
author Doghri, Maroua
Rodríguez, Víctor Manuel
Kliebenstein, Daniel J.
Francisco, Marta
author_facet Doghri, Maroua
Rodríguez, Víctor Manuel
Kliebenstein, Daniel J.
Francisco, Marta
author_sort Doghri, Maroua
collection PubMed
description A large subset of plant stress-signaling pathways, including those related with chemical defense production, exhibit diurnal or circadian oscillations. However the extent to which diurnal or circadian time influences the stress mediated accumulation of plant specialized metabolites remains largely unknown. Because plant responses to physical stress (e.g., wounding) is considered a common component of mounting a response against a broad range of environmental stresses, including herbivory, we have utilized mechanical wounding as the stress stimulus to determine the direct contribution of time of day on the induced defenses of Brassica crops. We analyzed glucosinolates (GSLs) from leaves of broccoli (Brassica oleracea) and turnip greens (Brassica rapa) following exposure to mechanical wounding at dawn (ZT0), mid-day (ZT4), and dusk (ZT8). Several GSLs differentially accumulated and their changes depended upon the time of day at wounding was performed. This response varied considerably between species. In a parallel experiment, we investigated whether diurnal activation of Brassica phytochemicals in response to wounding might prime plants against herbivore attack. Results showed that maximal response of plant chemical defense against larvae of the generalist pest Mamestra brassicae occurred at ZT0 in broccoli and ZT8 in turnip greens. Metabolome analysis for global trends of time dependent compounds showed that sulfur-containing phytochemicals, GSL hydrolysis products, auxin-signaling components, and other metabolites activators of plant disease resistance (nicotinamide and pipecolate) had important contributions to the responses of M. brassicae feeding behavior in broccoli at morning. Overall, the findings in this study highlight a significant role for time of day in the wound stress responsive metabolome, which can in turn affect plant-herbivore interactions.
format Online
Article
Text
id pubmed-8850993
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88509932022-02-18 Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops Doghri, Maroua Rodríguez, Víctor Manuel Kliebenstein, Daniel J. Francisco, Marta Front Plant Sci Plant Science A large subset of plant stress-signaling pathways, including those related with chemical defense production, exhibit diurnal or circadian oscillations. However the extent to which diurnal or circadian time influences the stress mediated accumulation of plant specialized metabolites remains largely unknown. Because plant responses to physical stress (e.g., wounding) is considered a common component of mounting a response against a broad range of environmental stresses, including herbivory, we have utilized mechanical wounding as the stress stimulus to determine the direct contribution of time of day on the induced defenses of Brassica crops. We analyzed glucosinolates (GSLs) from leaves of broccoli (Brassica oleracea) and turnip greens (Brassica rapa) following exposure to mechanical wounding at dawn (ZT0), mid-day (ZT4), and dusk (ZT8). Several GSLs differentially accumulated and their changes depended upon the time of day at wounding was performed. This response varied considerably between species. In a parallel experiment, we investigated whether diurnal activation of Brassica phytochemicals in response to wounding might prime plants against herbivore attack. Results showed that maximal response of plant chemical defense against larvae of the generalist pest Mamestra brassicae occurred at ZT0 in broccoli and ZT8 in turnip greens. Metabolome analysis for global trends of time dependent compounds showed that sulfur-containing phytochemicals, GSL hydrolysis products, auxin-signaling components, and other metabolites activators of plant disease resistance (nicotinamide and pipecolate) had important contributions to the responses of M. brassicae feeding behavior in broccoli at morning. Overall, the findings in this study highlight a significant role for time of day in the wound stress responsive metabolome, which can in turn affect plant-herbivore interactions. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8850993/ /pubmed/35185956 http://dx.doi.org/10.3389/fpls.2021.807710 Text en Copyright © 2022 Doghri, Rodríguez, Kliebenstein and Francisco. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Doghri, Maroua
Rodríguez, Víctor Manuel
Kliebenstein, Daniel J.
Francisco, Marta
Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title_full Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title_fullStr Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title_full_unstemmed Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title_short Plant Responses Underlying Timely Specialized Metabolites Induction of Brassica Crops
title_sort plant responses underlying timely specialized metabolites induction of brassica crops
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8850993/
https://www.ncbi.nlm.nih.gov/pubmed/35185956
http://dx.doi.org/10.3389/fpls.2021.807710
work_keys_str_mv AT doghrimaroua plantresponsesunderlyingtimelyspecializedmetabolitesinductionofbrassicacrops
AT rodriguezvictormanuel plantresponsesunderlyingtimelyspecializedmetabolitesinductionofbrassicacrops
AT kliebensteindanielj plantresponsesunderlyingtimelyspecializedmetabolitesinductionofbrassicacrops
AT franciscomarta plantresponsesunderlyingtimelyspecializedmetabolitesinductionofbrassicacrops