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The proteomic profile of the human myotendinous junction

Proteomics analysis of skeletal muscle has recently progressed from whole muscle tissue to single myofibers. Here, we further focus on a specific myofiber domain crucial for force transmission from muscle to tendon, the myotendinous junction (MTJ). To overcome the anatomical constraints preventing t...

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Autores principales: Karlsen, Anders, Gonzalez-Franquesa, Alba, Jakobsen, Jens R., Krogsgaard, Michael R., Koch, Manuel, Kjaer, Michael, Schiaffino, Stefano, Mackey, Abigail L., Deshmukh, Atul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851264/
https://www.ncbi.nlm.nih.gov/pubmed/35198892
http://dx.doi.org/10.1016/j.isci.2022.103836
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author Karlsen, Anders
Gonzalez-Franquesa, Alba
Jakobsen, Jens R.
Krogsgaard, Michael R.
Koch, Manuel
Kjaer, Michael
Schiaffino, Stefano
Mackey, Abigail L.
Deshmukh, Atul S.
author_facet Karlsen, Anders
Gonzalez-Franquesa, Alba
Jakobsen, Jens R.
Krogsgaard, Michael R.
Koch, Manuel
Kjaer, Michael
Schiaffino, Stefano
Mackey, Abigail L.
Deshmukh, Atul S.
author_sort Karlsen, Anders
collection PubMed
description Proteomics analysis of skeletal muscle has recently progressed from whole muscle tissue to single myofibers. Here, we further focus on a specific myofiber domain crucial for force transmission from muscle to tendon, the myotendinous junction (MTJ). To overcome the anatomical constraints preventing the isolation of pure MTJs, we performed in-depth analysis of the MTJ by progressive removal of the muscle component in semitendinosus muscle-tendon samples. Using detergents with increasing stringency, we quantified >3000 proteins across all samples, and identified 112 significantly enriched MTJ proteins, including 24 known MTJ-enriched proteins. Of the 88 novel MTJ markers, immunofluorescence analysis confirmed the presence of tetraspanin-24 (CD151), kindlin-2 (FERMT2), cartilage intermediate layer protein 1 (CILP), and integrin-alpha10 (ITGA10), at the human MTJ. Together, these human data constitute the first detailed MTJ proteomics resource that will contribute to advance understanding of the biology of the MTJ and its failure in pathological conditions.
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spelling pubmed-88512642022-02-22 The proteomic profile of the human myotendinous junction Karlsen, Anders Gonzalez-Franquesa, Alba Jakobsen, Jens R. Krogsgaard, Michael R. Koch, Manuel Kjaer, Michael Schiaffino, Stefano Mackey, Abigail L. Deshmukh, Atul S. iScience Article Proteomics analysis of skeletal muscle has recently progressed from whole muscle tissue to single myofibers. Here, we further focus on a specific myofiber domain crucial for force transmission from muscle to tendon, the myotendinous junction (MTJ). To overcome the anatomical constraints preventing the isolation of pure MTJs, we performed in-depth analysis of the MTJ by progressive removal of the muscle component in semitendinosus muscle-tendon samples. Using detergents with increasing stringency, we quantified >3000 proteins across all samples, and identified 112 significantly enriched MTJ proteins, including 24 known MTJ-enriched proteins. Of the 88 novel MTJ markers, immunofluorescence analysis confirmed the presence of tetraspanin-24 (CD151), kindlin-2 (FERMT2), cartilage intermediate layer protein 1 (CILP), and integrin-alpha10 (ITGA10), at the human MTJ. Together, these human data constitute the first detailed MTJ proteomics resource that will contribute to advance understanding of the biology of the MTJ and its failure in pathological conditions. Elsevier 2022-01-29 /pmc/articles/PMC8851264/ /pubmed/35198892 http://dx.doi.org/10.1016/j.isci.2022.103836 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Karlsen, Anders
Gonzalez-Franquesa, Alba
Jakobsen, Jens R.
Krogsgaard, Michael R.
Koch, Manuel
Kjaer, Michael
Schiaffino, Stefano
Mackey, Abigail L.
Deshmukh, Atul S.
The proteomic profile of the human myotendinous junction
title The proteomic profile of the human myotendinous junction
title_full The proteomic profile of the human myotendinous junction
title_fullStr The proteomic profile of the human myotendinous junction
title_full_unstemmed The proteomic profile of the human myotendinous junction
title_short The proteomic profile of the human myotendinous junction
title_sort proteomic profile of the human myotendinous junction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851264/
https://www.ncbi.nlm.nih.gov/pubmed/35198892
http://dx.doi.org/10.1016/j.isci.2022.103836
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