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Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage

The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determi...

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Autores principales: Nguyen, Dung Ho My, Ponjarat, Jatupong, Laopichienpong, Nararat, Panthum, Thitipong, Singchat, Worapong, Ahmad, Syed Farhan, Kraichak, Ekaphan, Muangmai, Narongrit, Duengkae, Prateep, Peyachoknagul, Surin, Na-Nakorn, Uthairat, Srikulnath, Kornsorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851383/
https://www.ncbi.nlm.nih.gov/pubmed/35186027
http://dx.doi.org/10.3389/fgene.2022.789573
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author Nguyen, Dung Ho My
Ponjarat, Jatupong
Laopichienpong, Nararat
Panthum, Thitipong
Singchat, Worapong
Ahmad, Syed Farhan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Peyachoknagul, Surin
Na-Nakorn, Uthairat
Srikulnath, Kornsorn
author_facet Nguyen, Dung Ho My
Ponjarat, Jatupong
Laopichienpong, Nararat
Panthum, Thitipong
Singchat, Worapong
Ahmad, Syed Farhan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Peyachoknagul, Surin
Na-Nakorn, Uthairat
Srikulnath, Kornsorn
author_sort Nguyen, Dung Ho My
collection PubMed
description The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determine sex within a species. Here, we aimed to detect the existence of PSD using hybrid. The hybrid produced by crossing male African catfish with female bighead catfish (C. macrocephalus, XX/XY) is a good animal model to study SDSs. Determining the SDS of hybrid catfish can help in understanding the interactions between these two complex SDS systems. Using the genotyping-by-sequencing “DART-seq” approach, we detected seven moderately male-linked loci and seventeen female-linked loci across all the examined hybrid specimens. Most of these loci were not sex-linked in the parental species, suggesting that the hybrid exhibits a combination of different alleles. Annotation of the identified sex-linked loci revealed the presence of one female-linked locus homologous with the B4GALNT1 gene, which is involved in the spermatogenesis pathway and hatchability. However, this locus was not sex-linked in the parental species, and the African catfish might also exhibit PSD.
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spelling pubmed-88513832022-02-18 Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage Nguyen, Dung Ho My Ponjarat, Jatupong Laopichienpong, Nararat Panthum, Thitipong Singchat, Worapong Ahmad, Syed Farhan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Peyachoknagul, Surin Na-Nakorn, Uthairat Srikulnath, Kornsorn Front Genet Genetics The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determine sex within a species. Here, we aimed to detect the existence of PSD using hybrid. The hybrid produced by crossing male African catfish with female bighead catfish (C. macrocephalus, XX/XY) is a good animal model to study SDSs. Determining the SDS of hybrid catfish can help in understanding the interactions between these two complex SDS systems. Using the genotyping-by-sequencing “DART-seq” approach, we detected seven moderately male-linked loci and seventeen female-linked loci across all the examined hybrid specimens. Most of these loci were not sex-linked in the parental species, suggesting that the hybrid exhibits a combination of different alleles. Annotation of the identified sex-linked loci revealed the presence of one female-linked locus homologous with the B4GALNT1 gene, which is involved in the spermatogenesis pathway and hatchability. However, this locus was not sex-linked in the parental species, and the African catfish might also exhibit PSD. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8851383/ /pubmed/35186027 http://dx.doi.org/10.3389/fgene.2022.789573 Text en Copyright © 2022 Nguyen, Ponjarat, Laopichienpong, Panthum, Singchat, Ahmad, Kraichak, Muangmai, Duengkae, Peyachoknagul, Na-Nakorn and Srikulnath. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Nguyen, Dung Ho My
Ponjarat, Jatupong
Laopichienpong, Nararat
Panthum, Thitipong
Singchat, Worapong
Ahmad, Syed Farhan
Kraichak, Ekaphan
Muangmai, Narongrit
Duengkae, Prateep
Peyachoknagul, Surin
Na-Nakorn, Uthairat
Srikulnath, Kornsorn
Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title_full Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title_fullStr Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title_full_unstemmed Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title_short Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
title_sort genome-wide snp analysis of hybrid clariid fish reflects the existence of polygenic sex-determination in the lineage
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851383/
https://www.ncbi.nlm.nih.gov/pubmed/35186027
http://dx.doi.org/10.3389/fgene.2022.789573
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