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Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage
The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851383/ https://www.ncbi.nlm.nih.gov/pubmed/35186027 http://dx.doi.org/10.3389/fgene.2022.789573 |
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author | Nguyen, Dung Ho My Ponjarat, Jatupong Laopichienpong, Nararat Panthum, Thitipong Singchat, Worapong Ahmad, Syed Farhan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Peyachoknagul, Surin Na-Nakorn, Uthairat Srikulnath, Kornsorn |
author_facet | Nguyen, Dung Ho My Ponjarat, Jatupong Laopichienpong, Nararat Panthum, Thitipong Singchat, Worapong Ahmad, Syed Farhan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Peyachoknagul, Surin Na-Nakorn, Uthairat Srikulnath, Kornsorn |
author_sort | Nguyen, Dung Ho My |
collection | PubMed |
description | The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determine sex within a species. Here, we aimed to detect the existence of PSD using hybrid. The hybrid produced by crossing male African catfish with female bighead catfish (C. macrocephalus, XX/XY) is a good animal model to study SDSs. Determining the SDS of hybrid catfish can help in understanding the interactions between these two complex SDS systems. Using the genotyping-by-sequencing “DART-seq” approach, we detected seven moderately male-linked loci and seventeen female-linked loci across all the examined hybrid specimens. Most of these loci were not sex-linked in the parental species, suggesting that the hybrid exhibits a combination of different alleles. Annotation of the identified sex-linked loci revealed the presence of one female-linked locus homologous with the B4GALNT1 gene, which is involved in the spermatogenesis pathway and hatchability. However, this locus was not sex-linked in the parental species, and the African catfish might also exhibit PSD. |
format | Online Article Text |
id | pubmed-8851383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88513832022-02-18 Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage Nguyen, Dung Ho My Ponjarat, Jatupong Laopichienpong, Nararat Panthum, Thitipong Singchat, Worapong Ahmad, Syed Farhan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Peyachoknagul, Surin Na-Nakorn, Uthairat Srikulnath, Kornsorn Front Genet Genetics The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, the SDS of African catfish might be influenced by a polygenic sex-determination (PSD) system, comprising multiple independently segregating sex “switch” loci to determine sex within a species. Here, we aimed to detect the existence of PSD using hybrid. The hybrid produced by crossing male African catfish with female bighead catfish (C. macrocephalus, XX/XY) is a good animal model to study SDSs. Determining the SDS of hybrid catfish can help in understanding the interactions between these two complex SDS systems. Using the genotyping-by-sequencing “DART-seq” approach, we detected seven moderately male-linked loci and seventeen female-linked loci across all the examined hybrid specimens. Most of these loci were not sex-linked in the parental species, suggesting that the hybrid exhibits a combination of different alleles. Annotation of the identified sex-linked loci revealed the presence of one female-linked locus homologous with the B4GALNT1 gene, which is involved in the spermatogenesis pathway and hatchability. However, this locus was not sex-linked in the parental species, and the African catfish might also exhibit PSD. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8851383/ /pubmed/35186027 http://dx.doi.org/10.3389/fgene.2022.789573 Text en Copyright © 2022 Nguyen, Ponjarat, Laopichienpong, Panthum, Singchat, Ahmad, Kraichak, Muangmai, Duengkae, Peyachoknagul, Na-Nakorn and Srikulnath. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Nguyen, Dung Ho My Ponjarat, Jatupong Laopichienpong, Nararat Panthum, Thitipong Singchat, Worapong Ahmad, Syed Farhan Kraichak, Ekaphan Muangmai, Narongrit Duengkae, Prateep Peyachoknagul, Surin Na-Nakorn, Uthairat Srikulnath, Kornsorn Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title | Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title_full | Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title_fullStr | Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title_full_unstemmed | Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title_short | Genome-Wide SNP Analysis of Hybrid Clariid Fish Reflects the Existence of Polygenic Sex-Determination in the Lineage |
title_sort | genome-wide snp analysis of hybrid clariid fish reflects the existence of polygenic sex-determination in the lineage |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851383/ https://www.ncbi.nlm.nih.gov/pubmed/35186027 http://dx.doi.org/10.3389/fgene.2022.789573 |
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