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Methamphetamine Downregulates the Sperm-Specific Calcium Channels Involved in Sperm Motility in Rats
[Image: see text] Calcium channels play essential roles in sperm motility. A family of sperm-specific cation channels including CatSper1–4 has been identified as voltage-dependent ion channels that act as sperm motility regulators. Methamphetamine is known to cause apoptosis in seminiferous tubules...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851642/ https://www.ncbi.nlm.nih.gov/pubmed/35187334 http://dx.doi.org/10.1021/acsomega.1c06242 |
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author | Allaeian Jahromi, Zahra Meshkibaf, Mohammad Hassan Naghdi, Majid Vahdati, Akbar Makoolati, Zohreh |
author_facet | Allaeian Jahromi, Zahra Meshkibaf, Mohammad Hassan Naghdi, Majid Vahdati, Akbar Makoolati, Zohreh |
author_sort | Allaeian Jahromi, Zahra |
collection | PubMed |
description | [Image: see text] Calcium channels play essential roles in sperm motility. A family of sperm-specific cation channels including CatSper1–4 has been identified as voltage-dependent ion channels that act as sperm motility regulators. Methamphetamine is known to cause apoptosis in seminiferous tubules and affect sperm quality. This research was conducted to investigate the effects of methamphetamine on expression of the CatSper family and Mvh genes. Thirty-six adult Wistar rats were divided into four groups of nine rats each: the control and experimental groups 1, 2, and 3. The control group received no solvents or drugs, but experimental groups 1, 2, and 3 were daily given 0.2 mL of a solution by gavage that contained 0.5, 1, and 2 mg of methamphetamine, respectively, for 45 days. The rats were then anesthetized, and one testis removed from each rat was used in a reverse transcription-polymerase chain reaction (RT-PCR). Analysis of variance (ANOVA) and Tukey’s posthoc test were used to analyze the data at the P < 0.05 significance level. Treatment with methamphetamine resulted in decreased testis and epididymis weights compared to the control rats. The results showed that the mRNA fold expression level of the CatSper family and Mvh genes decreased significantly in experimental groups compared to that in the control (P < 0.05). Methamphetamine decreased the expression levels of the CatSper and Mvh genes, and thus, it seemed that it can increase the probability of infertility through sperm motility reduction by lowering the expression levels of these genes. |
format | Online Article Text |
id | pubmed-8851642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88516422022-02-18 Methamphetamine Downregulates the Sperm-Specific Calcium Channels Involved in Sperm Motility in Rats Allaeian Jahromi, Zahra Meshkibaf, Mohammad Hassan Naghdi, Majid Vahdati, Akbar Makoolati, Zohreh ACS Omega [Image: see text] Calcium channels play essential roles in sperm motility. A family of sperm-specific cation channels including CatSper1–4 has been identified as voltage-dependent ion channels that act as sperm motility regulators. Methamphetamine is known to cause apoptosis in seminiferous tubules and affect sperm quality. This research was conducted to investigate the effects of methamphetamine on expression of the CatSper family and Mvh genes. Thirty-six adult Wistar rats were divided into four groups of nine rats each: the control and experimental groups 1, 2, and 3. The control group received no solvents or drugs, but experimental groups 1, 2, and 3 were daily given 0.2 mL of a solution by gavage that contained 0.5, 1, and 2 mg of methamphetamine, respectively, for 45 days. The rats were then anesthetized, and one testis removed from each rat was used in a reverse transcription-polymerase chain reaction (RT-PCR). Analysis of variance (ANOVA) and Tukey’s posthoc test were used to analyze the data at the P < 0.05 significance level. Treatment with methamphetamine resulted in decreased testis and epididymis weights compared to the control rats. The results showed that the mRNA fold expression level of the CatSper family and Mvh genes decreased significantly in experimental groups compared to that in the control (P < 0.05). Methamphetamine decreased the expression levels of the CatSper and Mvh genes, and thus, it seemed that it can increase the probability of infertility through sperm motility reduction by lowering the expression levels of these genes. American Chemical Society 2022-02-05 /pmc/articles/PMC8851642/ /pubmed/35187334 http://dx.doi.org/10.1021/acsomega.1c06242 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Allaeian Jahromi, Zahra Meshkibaf, Mohammad Hassan Naghdi, Majid Vahdati, Akbar Makoolati, Zohreh Methamphetamine Downregulates the Sperm-Specific Calcium Channels Involved in Sperm Motility in Rats |
title | Methamphetamine Downregulates the Sperm-Specific Calcium
Channels Involved in Sperm Motility in Rats |
title_full | Methamphetamine Downregulates the Sperm-Specific Calcium
Channels Involved in Sperm Motility in Rats |
title_fullStr | Methamphetamine Downregulates the Sperm-Specific Calcium
Channels Involved in Sperm Motility in Rats |
title_full_unstemmed | Methamphetamine Downregulates the Sperm-Specific Calcium
Channels Involved in Sperm Motility in Rats |
title_short | Methamphetamine Downregulates the Sperm-Specific Calcium
Channels Involved in Sperm Motility in Rats |
title_sort | methamphetamine downregulates the sperm-specific calcium
channels involved in sperm motility in rats |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851642/ https://www.ncbi.nlm.nih.gov/pubmed/35187334 http://dx.doi.org/10.1021/acsomega.1c06242 |
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