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Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions

Extracellular vesicles (EVs) are important elements of intercellular communication. A plethora of different, occasionally even opposite, physiologic and pathologic effects have been attributed to these vesicles in the last decade. A direct comparison of individual observations is however hampered by...

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Autores principales: Kolonics, Ferenc, Kajdácsi, Erika, Farkas, Veronika J., Veres, Dániel S., Khamari, Delaram, Kittel, Ágnes, Merchant, Michael L., McLeish, Kenneth R., Lőrincz, Ákos M., Ligeti, Erzsébet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851677/
https://www.ncbi.nlm.nih.gov/pubmed/32946637
http://dx.doi.org/10.1002/JLB.3A0320-210R
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author Kolonics, Ferenc
Kajdácsi, Erika
Farkas, Veronika J.
Veres, Dániel S.
Khamari, Delaram
Kittel, Ágnes
Merchant, Michael L.
McLeish, Kenneth R.
Lőrincz, Ákos M.
Ligeti, Erzsébet
author_facet Kolonics, Ferenc
Kajdácsi, Erika
Farkas, Veronika J.
Veres, Dániel S.
Khamari, Delaram
Kittel, Ágnes
Merchant, Michael L.
McLeish, Kenneth R.
Lőrincz, Ákos M.
Ligeti, Erzsébet
author_sort Kolonics, Ferenc
collection PubMed
description Extracellular vesicles (EVs) are important elements of intercellular communication. A plethora of different, occasionally even opposite, physiologic and pathologic effects have been attributed to these vesicles in the last decade. A direct comparison of individual observations is however hampered by the significant differences in the way of elicitation, collection, handling, and storage of the investigated vesicles. In the current work, we carried out a careful comparative study on 3, previously characterized types of EVs produced by neutrophilic granulocytes. We investigated in parallel the modulation of multiple blood-related cells and functions by medium-sized vesicles. We show that EVs released from resting neutrophils exert anti-inflammatory action by reducing production of reactive oxygen species (ROS) and cytokine release from neutrophils. In contrast, vesicles generated upon encounter of neutrophils with opsonized particles rather promote proinflammatory processes as they increase production of ROS and cytokine secretion from neutrophils and activate endothelial cells. EVs released from apoptosing cells were mainly active in promoting coagulation. We thus propose that EVs are “custom made,” acquiring selective capacities depending on environmental factors prevailing at the time of their biogenesis.
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spelling pubmed-88516772022-02-17 Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions Kolonics, Ferenc Kajdácsi, Erika Farkas, Veronika J. Veres, Dániel S. Khamari, Delaram Kittel, Ágnes Merchant, Michael L. McLeish, Kenneth R. Lőrincz, Ákos M. Ligeti, Erzsébet J Leukoc Biol Article Extracellular vesicles (EVs) are important elements of intercellular communication. A plethora of different, occasionally even opposite, physiologic and pathologic effects have been attributed to these vesicles in the last decade. A direct comparison of individual observations is however hampered by the significant differences in the way of elicitation, collection, handling, and storage of the investigated vesicles. In the current work, we carried out a careful comparative study on 3, previously characterized types of EVs produced by neutrophilic granulocytes. We investigated in parallel the modulation of multiple blood-related cells and functions by medium-sized vesicles. We show that EVs released from resting neutrophils exert anti-inflammatory action by reducing production of reactive oxygen species (ROS) and cytokine release from neutrophils. In contrast, vesicles generated upon encounter of neutrophils with opsonized particles rather promote proinflammatory processes as they increase production of ROS and cytokine secretion from neutrophils and activate endothelial cells. EVs released from apoptosing cells were mainly active in promoting coagulation. We thus propose that EVs are “custom made,” acquiring selective capacities depending on environmental factors prevailing at the time of their biogenesis. 2021-04 2020-09-18 /pmc/articles/PMC8851677/ /pubmed/32946637 http://dx.doi.org/10.1002/JLB.3A0320-210R Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Article
Kolonics, Ferenc
Kajdácsi, Erika
Farkas, Veronika J.
Veres, Dániel S.
Khamari, Delaram
Kittel, Ágnes
Merchant, Michael L.
McLeish, Kenneth R.
Lőrincz, Ákos M.
Ligeti, Erzsébet
Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title_full Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title_fullStr Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title_full_unstemmed Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title_short Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
title_sort neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851677/
https://www.ncbi.nlm.nih.gov/pubmed/32946637
http://dx.doi.org/10.1002/JLB.3A0320-210R
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