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Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
CONTEXT: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. OBJECTIVE: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. METHODS: Abdominal subcutaneous (SA) and omental visceral adipocytes (V...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851937/ https://www.ncbi.nlm.nih.gov/pubmed/34669916 http://dx.doi.org/10.1210/clinem/dgab756 |
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author | Hruska, Pavel Kucera, Jan Pekar, Matej Holéczy, Pavol Mazur, Miloslav Buzga, Marek Kuruczova, Daniela Lenart, Peter Fialova Kucerova, Jana Potesil, David Zdrahal, Zbynek Bienertova-Vasku, Julie |
author_facet | Hruska, Pavel Kucera, Jan Pekar, Matej Holéczy, Pavol Mazur, Miloslav Buzga, Marek Kuruczova, Daniela Lenart, Peter Fialova Kucerova, Jana Potesil, David Zdrahal, Zbynek Bienertova-Vasku, Julie |
author_sort | Hruska, Pavel |
collection | PubMed |
description | CONTEXT: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. OBJECTIVE: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. METHODS: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. RESULTS: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondrial energy metabolism and translational or biosynthetic activity is higher in VA. CONCLUSION: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity. |
format | Online Article Text |
id | pubmed-8851937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88519372022-02-18 Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes Hruska, Pavel Kucera, Jan Pekar, Matej Holéczy, Pavol Mazur, Miloslav Buzga, Marek Kuruczova, Daniela Lenart, Peter Fialova Kucerova, Jana Potesil, David Zdrahal, Zbynek Bienertova-Vasku, Julie J Clin Endocrinol Metab Clinical Research Article CONTEXT: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. OBJECTIVE: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. METHODS: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. RESULTS: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondrial energy metabolism and translational or biosynthetic activity is higher in VA. CONCLUSION: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity. Oxford University Press 2021-10-20 /pmc/articles/PMC8851937/ /pubmed/34669916 http://dx.doi.org/10.1210/clinem/dgab756 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Clinical Research Article Hruska, Pavel Kucera, Jan Pekar, Matej Holéczy, Pavol Mazur, Miloslav Buzga, Marek Kuruczova, Daniela Lenart, Peter Fialova Kucerova, Jana Potesil, David Zdrahal, Zbynek Bienertova-Vasku, Julie Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title | Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title_full | Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title_fullStr | Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title_full_unstemmed | Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title_short | Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes |
title_sort | proteomic signatures of human visceral and subcutaneous adipocytes |
topic | Clinical Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8851937/ https://www.ncbi.nlm.nih.gov/pubmed/34669916 http://dx.doi.org/10.1210/clinem/dgab756 |
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