A hyper-resolving polynomial aperture and its application in microscopy

BACKGROUND: A hyper-resolving aperture composed of a polynomial distribution is suggested. The point spread function (PSF) is computed and compared with that corresponding to linear, quadratic, and circular apertures. In addition, the influence of the number of zones on the PSF is discussed. An appl...

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Autor principal: Hamed, A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853206/
https://www.ncbi.nlm.nih.gov/pubmed/35194553
http://dx.doi.org/10.1186/s43088-022-00209-z
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author Hamed, A. M.
author_facet Hamed, A. M.
author_sort Hamed, A. M.
collection PubMed
description BACKGROUND: A hyper-resolving aperture composed of a polynomial distribution is suggested. The point spread function (PSF) is computed and compared with that corresponding to linear, quadratic, and circular apertures. In addition, the influence of the number of zones on the PSF is discussed. An application on confocal scanning laser microscope using Siemen’s star pattern as an object considering the polynomial apertures is given. RESULTS: We have made polynomial apertures using MATLAB code, and we tested the resolution from the computation of the cut-off spatial frequency obtained from the computation of the point spread function. CONCLUSIONS: We get compromised resolution and contrast for the polynomial apertures as compared with uniform circular apertures.
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spelling pubmed-88532062022-02-18 A hyper-resolving polynomial aperture and its application in microscopy Hamed, A. M. Beni Suef Univ J Basic Appl Sci Research BACKGROUND: A hyper-resolving aperture composed of a polynomial distribution is suggested. The point spread function (PSF) is computed and compared with that corresponding to linear, quadratic, and circular apertures. In addition, the influence of the number of zones on the PSF is discussed. An application on confocal scanning laser microscope using Siemen’s star pattern as an object considering the polynomial apertures is given. RESULTS: We have made polynomial apertures using MATLAB code, and we tested the resolution from the computation of the cut-off spatial frequency obtained from the computation of the point spread function. CONCLUSIONS: We get compromised resolution and contrast for the polynomial apertures as compared with uniform circular apertures. Springer Berlin Heidelberg 2022-02-14 2022 /pmc/articles/PMC8853206/ /pubmed/35194553 http://dx.doi.org/10.1186/s43088-022-00209-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Hamed, A. M.
A hyper-resolving polynomial aperture and its application in microscopy
title A hyper-resolving polynomial aperture and its application in microscopy
title_full A hyper-resolving polynomial aperture and its application in microscopy
title_fullStr A hyper-resolving polynomial aperture and its application in microscopy
title_full_unstemmed A hyper-resolving polynomial aperture and its application in microscopy
title_short A hyper-resolving polynomial aperture and its application in microscopy
title_sort hyper-resolving polynomial aperture and its application in microscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853206/
https://www.ncbi.nlm.nih.gov/pubmed/35194553
http://dx.doi.org/10.1186/s43088-022-00209-z
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