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Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum

Disease caused by Pepper yellow leaf curl virus (PepYLCV) is one of the greatest threats to pepper (Capsicum spp.) cultivation in the tropics and subtropics. Resistance to PepYLCV was previously identified in a few Capsicum accessions, but no resistance QTLs have been mapped. This study aimed to elu...

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Autores principales: Siddique, Muhammad Irfan, Lee, Joung-Ho, Ahn, Jung-Hwan, Kusumawardhani, Meirina Kartika, Safitri, Ramadhani, Harpenas, Asep, Kwon, Jin-Kyung, Kang, Byoung-Cheorl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853517/
https://www.ncbi.nlm.nih.gov/pubmed/35176091
http://dx.doi.org/10.1371/journal.pone.0264026
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author Siddique, Muhammad Irfan
Lee, Joung-Ho
Ahn, Jung-Hwan
Kusumawardhani, Meirina Kartika
Safitri, Ramadhani
Harpenas, Asep
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
author_facet Siddique, Muhammad Irfan
Lee, Joung-Ho
Ahn, Jung-Hwan
Kusumawardhani, Meirina Kartika
Safitri, Ramadhani
Harpenas, Asep
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
author_sort Siddique, Muhammad Irfan
collection PubMed
description Disease caused by Pepper yellow leaf curl virus (PepYLCV) is one of the greatest threats to pepper (Capsicum spp.) cultivation in the tropics and subtropics. Resistance to PepYLCV was previously identified in a few Capsicum accessions, but no resistance QTLs have been mapped. This study aimed to elucidate the genetics of PepYLCV resistance in C. annuum L. Augmented inoculation by the viruliferous whitefly Bemisia tabaci was used to evaluate parental lines and an F(2) segregating population derived from a cross between resistant C. annuum line LP97 and susceptible C. annuum line ECW30R. Final evaluation was performed six weeks after inoculation using a standardized 5-point scale (0 = no symptoms to 4 = very severe symptoms). A high-density linkage map was constructed using genotyping-by-sequencing (GBS) to identify single-nucleotide polymorphism (SNP) markers associated with PepYLCV resistance in the F(2) population. QTL analysis revealed three QTLs, peplcv-1, peplcv-7, and peplcv-12, on chromosomes P1, P7, and P12, respectively. Candidate genes associated with PepYLCV resistance in the QTL regions were inferred. In addition, single markers Chr7-LCV-7 and Chr12-LCV-12 derived from the QTLs were developed and validated in another F(2) population and in commercial varieties. This work thus provides not only information for mapping PepYLCV resistance loci in pepper but also forms the basis for future molecular analysis of genes involved in PepYLCV resistance.
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spelling pubmed-88535172022-02-18 Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum Siddique, Muhammad Irfan Lee, Joung-Ho Ahn, Jung-Hwan Kusumawardhani, Meirina Kartika Safitri, Ramadhani Harpenas, Asep Kwon, Jin-Kyung Kang, Byoung-Cheorl PLoS One Research Article Disease caused by Pepper yellow leaf curl virus (PepYLCV) is one of the greatest threats to pepper (Capsicum spp.) cultivation in the tropics and subtropics. Resistance to PepYLCV was previously identified in a few Capsicum accessions, but no resistance QTLs have been mapped. This study aimed to elucidate the genetics of PepYLCV resistance in C. annuum L. Augmented inoculation by the viruliferous whitefly Bemisia tabaci was used to evaluate parental lines and an F(2) segregating population derived from a cross between resistant C. annuum line LP97 and susceptible C. annuum line ECW30R. Final evaluation was performed six weeks after inoculation using a standardized 5-point scale (0 = no symptoms to 4 = very severe symptoms). A high-density linkage map was constructed using genotyping-by-sequencing (GBS) to identify single-nucleotide polymorphism (SNP) markers associated with PepYLCV resistance in the F(2) population. QTL analysis revealed three QTLs, peplcv-1, peplcv-7, and peplcv-12, on chromosomes P1, P7, and P12, respectively. Candidate genes associated with PepYLCV resistance in the QTL regions were inferred. In addition, single markers Chr7-LCV-7 and Chr12-LCV-12 derived from the QTLs were developed and validated in another F(2) population and in commercial varieties. This work thus provides not only information for mapping PepYLCV resistance loci in pepper but also forms the basis for future molecular analysis of genes involved in PepYLCV resistance. Public Library of Science 2022-02-17 /pmc/articles/PMC8853517/ /pubmed/35176091 http://dx.doi.org/10.1371/journal.pone.0264026 Text en © 2022 Siddique et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Siddique, Muhammad Irfan
Lee, Joung-Ho
Ahn, Jung-Hwan
Kusumawardhani, Meirina Kartika
Safitri, Ramadhani
Harpenas, Asep
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title_full Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title_fullStr Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title_full_unstemmed Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title_short Genotyping-by-sequencing-based QTL mapping reveals novel loci for Pepper yellow leaf curl virus (PepYLCV) resistance in Capsicum annuum
title_sort genotyping-by-sequencing-based qtl mapping reveals novel loci for pepper yellow leaf curl virus (pepylcv) resistance in capsicum annuum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853517/
https://www.ncbi.nlm.nih.gov/pubmed/35176091
http://dx.doi.org/10.1371/journal.pone.0264026
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