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Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka
Organized intestinal mucosal immune response appears to be restricted to tetrapods. In teleost fish, there is no evidence for the existence of a particular intestinal region that facilitates the interaction of antigen-presenting cells (APCs) and T cells, such as secondary lymphoid organs. Indeed, de...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853713/ https://www.ncbi.nlm.nih.gov/pubmed/35185906 http://dx.doi.org/10.3389/fimmu.2022.812899 |
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author | Aghaallaei, Narges Agarwal, Rashi Benjaminsen, Joergen Lust, Katharina Bajoghli, Baubak Wittbrodt, Joachim Feijoo, Carmen G. |
author_facet | Aghaallaei, Narges Agarwal, Rashi Benjaminsen, Joergen Lust, Katharina Bajoghli, Baubak Wittbrodt, Joachim Feijoo, Carmen G. |
author_sort | Aghaallaei, Narges |
collection | PubMed |
description | Organized intestinal mucosal immune response appears to be restricted to tetrapods. In teleost fish, there is no evidence for the existence of a particular intestinal region that facilitates the interaction of antigen-presenting cells (APCs) and T cells, such as secondary lymphoid organs. Indeed, despite their importance in the defense against pathogens, the location and manner of APC-T cell interaction within the fish gut is unknown. Here, using non-invasive live imaging of newly developed transgenic reporter lines, we addressed the spatial organization and behavior of APCs and T cells in the intestine of medaka fish both during homeostasis and inflammation. We report that Ccr9a(+) T cells are recruited to a band in the lamina propria next to the muscularis mucosa in which Ccl25-expressing cells are present. Ccr9a(+) T cells contact APCs for several minutes, in a process mediated by connexin 43. This type of interaction was observed in homeostasis and inflammation, with the interaction being longer and more frequent during inflammation. Thus, our results demonstrate that the mucosal immune response in the intestine of medaka is organized and endowed with a specific region with specialized microenvironment and function. |
format | Online Article Text |
id | pubmed-8853713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88537132022-02-18 Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka Aghaallaei, Narges Agarwal, Rashi Benjaminsen, Joergen Lust, Katharina Bajoghli, Baubak Wittbrodt, Joachim Feijoo, Carmen G. Front Immunol Immunology Organized intestinal mucosal immune response appears to be restricted to tetrapods. In teleost fish, there is no evidence for the existence of a particular intestinal region that facilitates the interaction of antigen-presenting cells (APCs) and T cells, such as secondary lymphoid organs. Indeed, despite their importance in the defense against pathogens, the location and manner of APC-T cell interaction within the fish gut is unknown. Here, using non-invasive live imaging of newly developed transgenic reporter lines, we addressed the spatial organization and behavior of APCs and T cells in the intestine of medaka fish both during homeostasis and inflammation. We report that Ccr9a(+) T cells are recruited to a band in the lamina propria next to the muscularis mucosa in which Ccl25-expressing cells are present. Ccr9a(+) T cells contact APCs for several minutes, in a process mediated by connexin 43. This type of interaction was observed in homeostasis and inflammation, with the interaction being longer and more frequent during inflammation. Thus, our results demonstrate that the mucosal immune response in the intestine of medaka is organized and endowed with a specific region with specialized microenvironment and function. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8853713/ /pubmed/35185906 http://dx.doi.org/10.3389/fimmu.2022.812899 Text en Copyright © 2022 Aghaallaei, Agarwal, Benjaminsen, Lust, Bajoghli, Wittbrodt and Feijoo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Aghaallaei, Narges Agarwal, Rashi Benjaminsen, Joergen Lust, Katharina Bajoghli, Baubak Wittbrodt, Joachim Feijoo, Carmen G. Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title | Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title_full | Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title_fullStr | Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title_full_unstemmed | Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title_short | Antigen-Presenting Cells and T Cells Interact in a Specific Area of the Intestinal Mucosa Defined by the Ccl25-Ccr9 Axis in Medaka |
title_sort | antigen-presenting cells and t cells interact in a specific area of the intestinal mucosa defined by the ccl25-ccr9 axis in medaka |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8853713/ https://www.ncbi.nlm.nih.gov/pubmed/35185906 http://dx.doi.org/10.3389/fimmu.2022.812899 |
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