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Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers

Gut barrier disruption is the initial pathogenesis of various diseases. We previously reported that dietary allicin improves tight junction proteins in the endoplasmic reticulum stressed jejunum. However, whether the allicin benefits the gut barrier within mycotoxin or endotoxin exposure is unknown....

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Autores principales: Gao, Jingxia, Song, Guanzhong, Shen, Haibo, Wu, Yiming, Zhao, Chongqi, Zhang, Zhuo, Jiang, Qian, Li, Xilong, Ma, Xiaokang, Tan, Bie, Yin, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854216/
https://www.ncbi.nlm.nih.gov/pubmed/35185936
http://dx.doi.org/10.3389/fimmu.2022.847861
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author Gao, Jingxia
Song, Guanzhong
Shen, Haibo
Wu, Yiming
Zhao, Chongqi
Zhang, Zhuo
Jiang, Qian
Li, Xilong
Ma, Xiaokang
Tan, Bie
Yin, Yulong
author_facet Gao, Jingxia
Song, Guanzhong
Shen, Haibo
Wu, Yiming
Zhao, Chongqi
Zhang, Zhuo
Jiang, Qian
Li, Xilong
Ma, Xiaokang
Tan, Bie
Yin, Yulong
author_sort Gao, Jingxia
collection PubMed
description Gut barrier disruption is the initial pathogenesis of various diseases. We previously reported that dietary allicin improves tight junction proteins in the endoplasmic reticulum stressed jejunum. However, whether the allicin benefits the gut barrier within mycotoxin or endotoxin exposure is unknown. In the present study, IPEC-J2 cell monolayers within or without deoxynivalenol (DON) or lipopolysaccharide (LPS) challenges were employed to investigate the effects of allicin on intestinal barrier function and explore the potential mechanisms. Results clarified that allicin at 2 μg/mL increased the viability, whereas the allicin higher than 10 μg/mL lowered the viability of IPEC-J2 cells via inhibiting cell proliferation. Besides, allicin increased trans-epithelial electric resistance (TEER), decreased paracellular permeability, and enhanced ZO-1 integrity of the IPEC-J2 cell monolayers. Finally, allicin supplementation prevented the LPS-induced barrier damages via activating Nrf2/HO-1 pathway-dependent antioxidant system. In conclusion, the present study strongly confirmed allicin as an effective nutrient to improve intestinal barrier function and prevent bacterial endotoxin-induced barrier damages.
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spelling pubmed-88542162022-02-19 Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers Gao, Jingxia Song, Guanzhong Shen, Haibo Wu, Yiming Zhao, Chongqi Zhang, Zhuo Jiang, Qian Li, Xilong Ma, Xiaokang Tan, Bie Yin, Yulong Front Immunol Immunology Gut barrier disruption is the initial pathogenesis of various diseases. We previously reported that dietary allicin improves tight junction proteins in the endoplasmic reticulum stressed jejunum. However, whether the allicin benefits the gut barrier within mycotoxin or endotoxin exposure is unknown. In the present study, IPEC-J2 cell monolayers within or without deoxynivalenol (DON) or lipopolysaccharide (LPS) challenges were employed to investigate the effects of allicin on intestinal barrier function and explore the potential mechanisms. Results clarified that allicin at 2 μg/mL increased the viability, whereas the allicin higher than 10 μg/mL lowered the viability of IPEC-J2 cells via inhibiting cell proliferation. Besides, allicin increased trans-epithelial electric resistance (TEER), decreased paracellular permeability, and enhanced ZO-1 integrity of the IPEC-J2 cell monolayers. Finally, allicin supplementation prevented the LPS-induced barrier damages via activating Nrf2/HO-1 pathway-dependent antioxidant system. In conclusion, the present study strongly confirmed allicin as an effective nutrient to improve intestinal barrier function and prevent bacterial endotoxin-induced barrier damages. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854216/ /pubmed/35185936 http://dx.doi.org/10.3389/fimmu.2022.847861 Text en Copyright © 2022 Gao, Song, Shen, Wu, Zhao, Zhang, Jiang, Li, Ma, Tan and Yin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Gao, Jingxia
Song, Guanzhong
Shen, Haibo
Wu, Yiming
Zhao, Chongqi
Zhang, Zhuo
Jiang, Qian
Li, Xilong
Ma, Xiaokang
Tan, Bie
Yin, Yulong
Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title_full Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title_fullStr Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title_full_unstemmed Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title_short Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers
title_sort allicin improves intestinal epithelial barrier function and prevents lps-induced barrier damages of intestinal epithelial cell monolayers
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854216/
https://www.ncbi.nlm.nih.gov/pubmed/35185936
http://dx.doi.org/10.3389/fimmu.2022.847861
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