Cargando…
Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution
Species of the ciliate genus Urotricha are key players in freshwater plankton communities. In the pelagial of lakes, about 20 urotrich species occur throughout an annual cycle, some of which play a pivotal role in aquatic food webs. For example, during the phytoplankton spring bloom, they consume a...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854374/ https://www.ncbi.nlm.nih.gov/pubmed/35185817 http://dx.doi.org/10.3389/fmicb.2021.787290 |
_version_ | 1784653431180361728 |
---|---|
author | Frantal, Daniela Agatha, Sabine Beisser, Daniela Boenigk, Jens Darienko, Tatyana Dirren-Pitsch, Gianna Filker, Sabine Gruber, Michael Kammerlander, Barbara Nachbaur, Laura Scheffel, Ulrike Stoeck, Thorsten Qian, Kuimei Weißenbacher, Birgit Pröschold, Thomas Sonntag, Bettina |
author_facet | Frantal, Daniela Agatha, Sabine Beisser, Daniela Boenigk, Jens Darienko, Tatyana Dirren-Pitsch, Gianna Filker, Sabine Gruber, Michael Kammerlander, Barbara Nachbaur, Laura Scheffel, Ulrike Stoeck, Thorsten Qian, Kuimei Weißenbacher, Birgit Pröschold, Thomas Sonntag, Bettina |
author_sort | Frantal, Daniela |
collection | PubMed |
description | Species of the ciliate genus Urotricha are key players in freshwater plankton communities. In the pelagial of lakes, about 20 urotrich species occur throughout an annual cycle, some of which play a pivotal role in aquatic food webs. For example, during the phytoplankton spring bloom, they consume a remarkable proportion of the algal production. In ecological studies, urotrich ciliates are usually merely identified to genus rank and grouped into size classes. This is unsatisfying considering the distinct autecological properties of individual species and their specific spatial and temporal distribution patterns. As a basis for future research, we characterized in detail four common urotrich morphotypes, i.e., specimens identified as U. furcata and tentatively as U. agilis, U. pseudofurcata, and U. castalia, using state-of-the-art methods. We used an integrative polyphasic approach, in which morphological studies (in vivo observation, silver staining methods, scanning electron microscopy) were linked with a molecular approach exploiting four different gene fragments as taxonomic DNA barcodes with different resolution potential (SSU rDNA, ITS-1, ITS-2, hypervariable V4 and V9 regions of the SSU rDNA). We shed light on the diversity of urotrich ciliates as well as on their global distribution patterns, and annual cycles. Additionally, we coupled individual species occurrences and environmental parameters, and subsequently modeled the distribution and occurrence, using logistic regressions. Furthermore, for one strain putatively identified as U. castalia, we ascertained the optimal cultivation media and food preferences. Thereby, our comprehensive view on these important freshwater ciliates that frequently occur in environmental high throughput sequencing datasets worldwide will allow future studies to better exploit protistan plankton data from lakes. |
format | Online Article Text |
id | pubmed-8854374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88543742022-02-19 Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution Frantal, Daniela Agatha, Sabine Beisser, Daniela Boenigk, Jens Darienko, Tatyana Dirren-Pitsch, Gianna Filker, Sabine Gruber, Michael Kammerlander, Barbara Nachbaur, Laura Scheffel, Ulrike Stoeck, Thorsten Qian, Kuimei Weißenbacher, Birgit Pröschold, Thomas Sonntag, Bettina Front Microbiol Microbiology Species of the ciliate genus Urotricha are key players in freshwater plankton communities. In the pelagial of lakes, about 20 urotrich species occur throughout an annual cycle, some of which play a pivotal role in aquatic food webs. For example, during the phytoplankton spring bloom, they consume a remarkable proportion of the algal production. In ecological studies, urotrich ciliates are usually merely identified to genus rank and grouped into size classes. This is unsatisfying considering the distinct autecological properties of individual species and their specific spatial and temporal distribution patterns. As a basis for future research, we characterized in detail four common urotrich morphotypes, i.e., specimens identified as U. furcata and tentatively as U. agilis, U. pseudofurcata, and U. castalia, using state-of-the-art methods. We used an integrative polyphasic approach, in which morphological studies (in vivo observation, silver staining methods, scanning electron microscopy) were linked with a molecular approach exploiting four different gene fragments as taxonomic DNA barcodes with different resolution potential (SSU rDNA, ITS-1, ITS-2, hypervariable V4 and V9 regions of the SSU rDNA). We shed light on the diversity of urotrich ciliates as well as on their global distribution patterns, and annual cycles. Additionally, we coupled individual species occurrences and environmental parameters, and subsequently modeled the distribution and occurrence, using logistic regressions. Furthermore, for one strain putatively identified as U. castalia, we ascertained the optimal cultivation media and food preferences. Thereby, our comprehensive view on these important freshwater ciliates that frequently occur in environmental high throughput sequencing datasets worldwide will allow future studies to better exploit protistan plankton data from lakes. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854374/ /pubmed/35185817 http://dx.doi.org/10.3389/fmicb.2021.787290 Text en Copyright © 2022 Frantal, Agatha, Beisser, Boenigk, Darienko, Dirren-Pitsch, Filker, Gruber, Kammerlander, Nachbaur, Scheffel, Stoeck, Qian, Weißenbacher, Pröschold and Sonntag. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Frantal, Daniela Agatha, Sabine Beisser, Daniela Boenigk, Jens Darienko, Tatyana Dirren-Pitsch, Gianna Filker, Sabine Gruber, Michael Kammerlander, Barbara Nachbaur, Laura Scheffel, Ulrike Stoeck, Thorsten Qian, Kuimei Weißenbacher, Birgit Pröschold, Thomas Sonntag, Bettina Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title | Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title_full | Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title_fullStr | Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title_full_unstemmed | Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title_short | Molecular Data Reveal a Cryptic Diversity in the Genus Urotricha (Alveolata, Ciliophora, Prostomatida), a Key Player in Freshwater Lakes, With Remarks on Morphology, Food Preferences, and Distribution |
title_sort | molecular data reveal a cryptic diversity in the genus urotricha (alveolata, ciliophora, prostomatida), a key player in freshwater lakes, with remarks on morphology, food preferences, and distribution |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854374/ https://www.ncbi.nlm.nih.gov/pubmed/35185817 http://dx.doi.org/10.3389/fmicb.2021.787290 |
work_keys_str_mv | AT frantaldaniela moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT agathasabine moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT beisserdaniela moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT boenigkjens moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT darienkotatyana moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT dirrenpitschgianna moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT filkersabine moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT grubermichael moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT kammerlanderbarbara moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT nachbaurlaura moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT scheffelulrike moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT stoeckthorsten moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT qiankuimei moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT weißenbacherbirgit moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT proscholdthomas moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution AT sonntagbettina moleculardatarevealacrypticdiversityinthegenusurotrichaalveolataciliophoraprostomatidaakeyplayerinfreshwaterlakeswithremarksonmorphologyfoodpreferencesanddistribution |