Cargando…

Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies

As vaccination against SARS-CoV-2 progresses rapidly around the world, reliable detection of SARS-CoV-2 specific neutralizing antibodies (NAb) has become an indispensable component of serological diagnostics. We evaluated the performance of four commercially available tests, i.e. two lateral flow as...

Descripción completa

Detalles Bibliográficos
Autores principales: Girl, Philipp, Zwirglmaier, Katrin, von Buttlar, Heiner, Wölfel, Roman, Müller, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854747/
https://www.ncbi.nlm.nih.gov/pubmed/35187003
http://dx.doi.org/10.3389/fmed.2022.820151
_version_ 1784653497471336448
author Girl, Philipp
Zwirglmaier, Katrin
von Buttlar, Heiner
Wölfel, Roman
Müller, Katharina
author_facet Girl, Philipp
Zwirglmaier, Katrin
von Buttlar, Heiner
Wölfel, Roman
Müller, Katharina
author_sort Girl, Philipp
collection PubMed
description As vaccination against SARS-CoV-2 progresses rapidly around the world, reliable detection of SARS-CoV-2 specific neutralizing antibodies (NAb) has become an indispensable component of serological diagnostics. We evaluated the performance of four commercially available tests, i.e. two lateral flow assays (Coris BioConcept COVID-19 Sero NP/RBD and Concile InfectCheck COVID-19 NAb) and two surrogate ELISA (sELISA) tests (EUROIMMUN SARS-CoV-2 NeutraLISA and AdipoGen SARS-CoV-2 Neutralizing Antibodies Detection Kit) in comparison with an in-house SARS-CoV-2 micro neutralization test as reference. A total of 334 sera were tested, including 30 samples collected prior to the emergence of SARS-CoV-2, 128 sera from convalescent patients as well as 176 sera from partially or fully vaccinated individuals. The overall sensitivity of LFAs differed and was 71.6% for the Coris and 98.4% for the Concile. In contrast, overall sensitivity of the NeutraLISA was 86 and 98% for the AdipoGen. All test showed the highest sensitivity when testing samples from fully vaccinated individuals with both sELISA achieving 100% sensitivity. Overall specificity was 89.3% for the Coris and only 58.3% for the Concile. Similarly, significant differences were observed for both sELISA, with an overall specificity of 82.1% for the NeutraLISA and only 54.8% for the AdipoGen. All tests showed a 100% specificity when testing negative control samples while specificities were lowest when testing samples from only partially vaccinated individuals.
format Online
Article
Text
id pubmed-8854747
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88547472022-02-19 Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies Girl, Philipp Zwirglmaier, Katrin von Buttlar, Heiner Wölfel, Roman Müller, Katharina Front Med (Lausanne) Medicine As vaccination against SARS-CoV-2 progresses rapidly around the world, reliable detection of SARS-CoV-2 specific neutralizing antibodies (NAb) has become an indispensable component of serological diagnostics. We evaluated the performance of four commercially available tests, i.e. two lateral flow assays (Coris BioConcept COVID-19 Sero NP/RBD and Concile InfectCheck COVID-19 NAb) and two surrogate ELISA (sELISA) tests (EUROIMMUN SARS-CoV-2 NeutraLISA and AdipoGen SARS-CoV-2 Neutralizing Antibodies Detection Kit) in comparison with an in-house SARS-CoV-2 micro neutralization test as reference. A total of 334 sera were tested, including 30 samples collected prior to the emergence of SARS-CoV-2, 128 sera from convalescent patients as well as 176 sera from partially or fully vaccinated individuals. The overall sensitivity of LFAs differed and was 71.6% for the Coris and 98.4% for the Concile. In contrast, overall sensitivity of the NeutraLISA was 86 and 98% for the AdipoGen. All test showed the highest sensitivity when testing samples from fully vaccinated individuals with both sELISA achieving 100% sensitivity. Overall specificity was 89.3% for the Coris and only 58.3% for the Concile. Similarly, significant differences were observed for both sELISA, with an overall specificity of 82.1% for the NeutraLISA and only 54.8% for the AdipoGen. All tests showed a 100% specificity when testing negative control samples while specificities were lowest when testing samples from only partially vaccinated individuals. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854747/ /pubmed/35187003 http://dx.doi.org/10.3389/fmed.2022.820151 Text en Copyright © 2022 Girl, Zwirglmaier, von Buttlar, Wölfel and Müller. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Girl, Philipp
Zwirglmaier, Katrin
von Buttlar, Heiner
Wölfel, Roman
Müller, Katharina
Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title_full Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title_fullStr Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title_full_unstemmed Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title_short Evaluation of Two Rapid Lateral Flow Tests and Two Surrogate ELISAs for the Detection of SARS-CoV-2 Specific Neutralizing Antibodies
title_sort evaluation of two rapid lateral flow tests and two surrogate elisas for the detection of sars-cov-2 specific neutralizing antibodies
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854747/
https://www.ncbi.nlm.nih.gov/pubmed/35187003
http://dx.doi.org/10.3389/fmed.2022.820151
work_keys_str_mv AT girlphilipp evaluationoftworapidlateralflowtestsandtwosurrogateelisasforthedetectionofsarscov2specificneutralizingantibodies
AT zwirglmaierkatrin evaluationoftworapidlateralflowtestsandtwosurrogateelisasforthedetectionofsarscov2specificneutralizingantibodies
AT vonbuttlarheiner evaluationoftworapidlateralflowtestsandtwosurrogateelisasforthedetectionofsarscov2specificneutralizingantibodies
AT wolfelroman evaluationoftworapidlateralflowtestsandtwosurrogateelisasforthedetectionofsarscov2specificneutralizingantibodies
AT mullerkatharina evaluationoftworapidlateralflowtestsandtwosurrogateelisasforthedetectionofsarscov2specificneutralizingantibodies