Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to...

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Detalles Bibliográficos
Autores principales: Xing, Fanfan, Lo, Simon K. F., Ma, Yuanchao, Ip, Jonathan Daniel, Chan, Wan-Mui, Zhou, Meixun, Gong, Miaozi, Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854760/
https://www.ncbi.nlm.nih.gov/pubmed/35187006
http://dx.doi.org/10.3389/fmed.2022.824122
Descripción
Sumario:We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. NGS is particularly useful for culture-negative and slow-growing microorganism infections, such as mycobacterial, fungal and partially treated pyogenic bacterial infections. Due to its low equipment cost, short turn-around-time and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.

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