Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report

We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to...

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Autores principales: Xing, Fanfan, Lo, Simon K. F., Ma, Yuanchao, Ip, Jonathan Daniel, Chan, Wan-Mui, Zhou, Meixun, Gong, Miaozi, Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854760/
https://www.ncbi.nlm.nih.gov/pubmed/35187006
http://dx.doi.org/10.3389/fmed.2022.824122
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author Xing, Fanfan
Lo, Simon K. F.
Ma, Yuanchao
Ip, Jonathan Daniel
Chan, Wan-Mui
Zhou, Meixun
Gong, Miaozi
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_facet Xing, Fanfan
Lo, Simon K. F.
Ma, Yuanchao
Ip, Jonathan Daniel
Chan, Wan-Mui
Zhou, Meixun
Gong, Miaozi
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_sort Xing, Fanfan
collection PubMed
description We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. NGS is particularly useful for culture-negative and slow-growing microorganism infections, such as mycobacterial, fungal and partially treated pyogenic bacterial infections. Due to its low equipment cost, short turn-around-time and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.
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spelling pubmed-88547602022-02-19 Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report Xing, Fanfan Lo, Simon K. F. Ma, Yuanchao Ip, Jonathan Daniel Chan, Wan-Mui Zhou, Meixun Gong, Miaozi Lau, Susanna K. P. Woo, Patrick C. Y. Front Med (Lausanne) Medicine We present the first report of histology- and culture-proven Mycobacterium marinum infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. NGS is particularly useful for culture-negative and slow-growing microorganism infections, such as mycobacterial, fungal and partially treated pyogenic bacterial infections. Due to its low equipment cost, short turn-around-time and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854760/ /pubmed/35187006 http://dx.doi.org/10.3389/fmed.2022.824122 Text en Copyright © 2022 Xing, Lo, Ma, Ip, Chan, Zhou, Gong, Lau and Woo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Xing, Fanfan
Lo, Simon K. F.
Ma, Yuanchao
Ip, Jonathan Daniel
Chan, Wan-Mui
Zhou, Meixun
Gong, Miaozi
Lau, Susanna K. P.
Woo, Patrick C. Y.
Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title_full Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title_fullStr Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title_full_unstemmed Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title_short Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
title_sort rapid diagnosis of mycobacterium marinum infection by next-generation sequencing: a case report
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854760/
https://www.ncbi.nlm.nih.gov/pubmed/35187006
http://dx.doi.org/10.3389/fmed.2022.824122
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