Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16

The hydroxyapatite (HA) coating on carbon/carbon (C/C) is reasonable and feasible to obtain bone graft materials with appropriate mechanical and biological properties. However, improvement of the physical and chemical properties of HA-C/C composites to promote bone regeneration and healing remains a...

Descripción completa

Detalles Bibliográficos
Autores principales: Qi, Hong, Liu, Yang, Wu, Lu, Liu, Chun, Ni, Su, Liu, Qizhan, Ni, Xinye, Sun, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854763/
https://www.ncbi.nlm.nih.gov/pubmed/35186909
http://dx.doi.org/10.3389/fbioe.2022.838842
_version_ 1784653501372039168
author Qi, Hong
Liu, Yang
Wu, Lu
Liu, Chun
Ni, Su
Liu, Qizhan
Ni, Xinye
Sun, Qiang
author_facet Qi, Hong
Liu, Yang
Wu, Lu
Liu, Chun
Ni, Su
Liu, Qizhan
Ni, Xinye
Sun, Qiang
author_sort Qi, Hong
collection PubMed
description The hydroxyapatite (HA) coating on carbon/carbon (C/C) is reasonable and feasible to obtain bone graft materials with appropriate mechanical and biological properties. However, improvement of the physical and chemical properties of HA-C/C composites to promote bone regeneration and healing remains a challenge. In our present study, the HA coatings on C/C with magnesium (Mg) (Mg-HA-C/C) composites were synthesized that Ca (NO(3))(2), Mg (NO(3))(2), and NH(4)H(2)PO(4) were mixed and coatings were made by electromagnetic induction deposition’s heating. As determined with in vitro experiments, Mg-HA-C/C composites containing 10 and 20% Mg decreased miR-16 levels, increased cell viability, elevated the levels of osteogenesis-related genes, and promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) seeded on their surfaces. In a rat model of skull defects, compared to the control group, at 4 and 12 weeks after the operation, the bone volume fraction (BV/TV) of Mg-HA-C/C composite group was increased by 8.439 ± 2.681% and 23.837 ± 7.845%, as well as the trabecular thickness (Tb.Th) was 56.247 ± 24.238 μm and 114.911 ± 34.015 μm more. These composites also increased the levels of ALP and RUNX2 in skull. The Mg-HA-C/C composite-enhanced bone regeneration and healing were blocked by in situ injection of an miR-16 mimic lentivirus vector. Thus, Mg-HA-C/C composites promote osteogenic differentiation and repair bone defects through inhibiting miR-16.
format Online
Article
Text
id pubmed-8854763
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88547632022-02-19 Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16 Qi, Hong Liu, Yang Wu, Lu Liu, Chun Ni, Su Liu, Qizhan Ni, Xinye Sun, Qiang Front Bioeng Biotechnol Bioengineering and Biotechnology The hydroxyapatite (HA) coating on carbon/carbon (C/C) is reasonable and feasible to obtain bone graft materials with appropriate mechanical and biological properties. However, improvement of the physical and chemical properties of HA-C/C composites to promote bone regeneration and healing remains a challenge. In our present study, the HA coatings on C/C with magnesium (Mg) (Mg-HA-C/C) composites were synthesized that Ca (NO(3))(2), Mg (NO(3))(2), and NH(4)H(2)PO(4) were mixed and coatings were made by electromagnetic induction deposition’s heating. As determined with in vitro experiments, Mg-HA-C/C composites containing 10 and 20% Mg decreased miR-16 levels, increased cell viability, elevated the levels of osteogenesis-related genes, and promoted osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) seeded on their surfaces. In a rat model of skull defects, compared to the control group, at 4 and 12 weeks after the operation, the bone volume fraction (BV/TV) of Mg-HA-C/C composite group was increased by 8.439 ± 2.681% and 23.837 ± 7.845%, as well as the trabecular thickness (Tb.Th) was 56.247 ± 24.238 μm and 114.911 ± 34.015 μm more. These composites also increased the levels of ALP and RUNX2 in skull. The Mg-HA-C/C composite-enhanced bone regeneration and healing were blocked by in situ injection of an miR-16 mimic lentivirus vector. Thus, Mg-HA-C/C composites promote osteogenic differentiation and repair bone defects through inhibiting miR-16. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854763/ /pubmed/35186909 http://dx.doi.org/10.3389/fbioe.2022.838842 Text en Copyright © 2022 Qi, Liu, Wu, Liu, Ni, Liu, Ni and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Qi, Hong
Liu, Yang
Wu, Lu
Liu, Chun
Ni, Su
Liu, Qizhan
Ni, Xinye
Sun, Qiang
Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title_full Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title_fullStr Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title_full_unstemmed Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title_short Mg-HA-C/C Composites Promote Osteogenic Differentiation and Repair Bone Defects Through Inhibiting miR-16
title_sort mg-ha-c/c composites promote osteogenic differentiation and repair bone defects through inhibiting mir-16
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854763/
https://www.ncbi.nlm.nih.gov/pubmed/35186909
http://dx.doi.org/10.3389/fbioe.2022.838842
work_keys_str_mv AT qihong mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT liuyang mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT wulu mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT liuchun mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT nisu mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT liuqizhan mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT nixinye mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16
AT sunqiang mghacccompositespromoteosteogenicdifferentiationandrepairbonedefectsthroughinhibitingmir16

Ejemplares similares