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The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra

PURPOSE: The vasodilatory response to inhaled CO(2) occurs in the acute stroke ischemic penumbra and may be a potential therapeutic modality. METHODS: Twenty-two Sprague-Dawley rats were subjected to 90-min occlusion of the M2 segment of the middle cerebral artery (M2CAO) by endovascular technique....

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Autores principales: Yeo, Leonard L., Arnberg, Fabian, Chireh, Arvin, Sharma, Vijay, Tan, Benjamin, Gontu, Vamsi, Little, Philip, Holmin, Staffan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854855/
https://www.ncbi.nlm.nih.gov/pubmed/35187526
http://dx.doi.org/10.3389/fdgth.2021.824334
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author Yeo, Leonard L.
Arnberg, Fabian
Chireh, Arvin
Sharma, Vijay
Tan, Benjamin
Gontu, Vamsi
Little, Philip
Holmin, Staffan
author_facet Yeo, Leonard L.
Arnberg, Fabian
Chireh, Arvin
Sharma, Vijay
Tan, Benjamin
Gontu, Vamsi
Little, Philip
Holmin, Staffan
author_sort Yeo, Leonard L.
collection PubMed
description PURPOSE: The vasodilatory response to inhaled CO(2) occurs in the acute stroke ischemic penumbra and may be a potential therapeutic modality. METHODS: Twenty-two Sprague-Dawley rats were subjected to 90-min occlusion of the M2 segment of the middle cerebral artery (M2CAO) by endovascular technique. The animals were administered different C02 concentrations and scanned serially with 9.4 T MRI. Infarct tissue was determined by diffusion-weighted imaging (DWI) and hypoperfused tissue was determined by arterial spin labeling (PWI). RESULTS: 4 animals were administered room air (RA)+ 6% CO(2) (group 1), 6 animals RA+12% CO(2) (Group 2) and 4 animals only RA (group 3). In the rats with CO(2) administered (groups 1 and 2), the DWI lesion to cerebral hypoperfusion volume ratio (SD) at pre-CO(2) administration, was 0.145(0.168), which increased to 0.708(0.731) during CO(2) administration and reduced to 0.533(0.527) post-CO(2) administration. In 9 of 10 rats the hypoperfused volume decreased when CO(2) was administered. When CO(2) was stopped the hypoperfused volume became larger again. Administration of RA+12% CO(2) (Group 2) decreased the volume of CBF hypoperfusion significantly compared to the control group (95%CI: 0.084 ± 0.0213, p = 0.004). CONCLUSION: Inhaled CO(2) appears to reduce the size of the hypoperfused tissue volume during acute stroke and may be a potential modality for treatment of acute ischemic stroke. These findings will nonetheless need to be validated in a larger cohort in other centers.
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spelling pubmed-88548552022-02-19 The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra Yeo, Leonard L. Arnberg, Fabian Chireh, Arvin Sharma, Vijay Tan, Benjamin Gontu, Vamsi Little, Philip Holmin, Staffan Front Digit Health Digital Health PURPOSE: The vasodilatory response to inhaled CO(2) occurs in the acute stroke ischemic penumbra and may be a potential therapeutic modality. METHODS: Twenty-two Sprague-Dawley rats were subjected to 90-min occlusion of the M2 segment of the middle cerebral artery (M2CAO) by endovascular technique. The animals were administered different C02 concentrations and scanned serially with 9.4 T MRI. Infarct tissue was determined by diffusion-weighted imaging (DWI) and hypoperfused tissue was determined by arterial spin labeling (PWI). RESULTS: 4 animals were administered room air (RA)+ 6% CO(2) (group 1), 6 animals RA+12% CO(2) (Group 2) and 4 animals only RA (group 3). In the rats with CO(2) administered (groups 1 and 2), the DWI lesion to cerebral hypoperfusion volume ratio (SD) at pre-CO(2) administration, was 0.145(0.168), which increased to 0.708(0.731) during CO(2) administration and reduced to 0.533(0.527) post-CO(2) administration. In 9 of 10 rats the hypoperfused volume decreased when CO(2) was administered. When CO(2) was stopped the hypoperfused volume became larger again. Administration of RA+12% CO(2) (Group 2) decreased the volume of CBF hypoperfusion significantly compared to the control group (95%CI: 0.084 ± 0.0213, p = 0.004). CONCLUSION: Inhaled CO(2) appears to reduce the size of the hypoperfused tissue volume during acute stroke and may be a potential modality for treatment of acute ischemic stroke. These findings will nonetheless need to be validated in a larger cohort in other centers. Frontiers Media S.A. 2022-02-04 /pmc/articles/PMC8854855/ /pubmed/35187526 http://dx.doi.org/10.3389/fdgth.2021.824334 Text en Copyright © 2022 Yeo, Arnberg, Chireh, Sharma, Tan, Gontu, Little and Holmin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Digital Health
Yeo, Leonard L.
Arnberg, Fabian
Chireh, Arvin
Sharma, Vijay
Tan, Benjamin
Gontu, Vamsi
Little, Philip
Holmin, Staffan
The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title_full The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title_fullStr The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title_full_unstemmed The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title_short The Role of Carbon Dioxide in the Rat Acute Stroke Penumbra
title_sort role of carbon dioxide in the rat acute stroke penumbra
topic Digital Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8854855/
https://www.ncbi.nlm.nih.gov/pubmed/35187526
http://dx.doi.org/10.3389/fdgth.2021.824334
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